Dual-Chamber Micro Cross-Section Detector for Permanent Gas Analysis.

Abel, Kenneth; deSchmertzing, Hannibal.

doi:10.1021/ac60204a064

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…Diebolt (46) reports the results obtained in a study in three parts: (1) The rare gases were separated on activated carbon at constant pressure by a programmed increase of column temperature and determined by counting of radioactivity (125). Xe not previously used for radioactive detection of Xe provides very good sensitivity.…”

Section: Gas Analysismentioning

confidence: 99%

Gas Analysis

Hobbs¹

1966

Anal. Chem.

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Section: Gas Analysismentioning

confidence: 99%

Gas Analysis

Hobbs¹

1966

Anal. Chem.

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“…While the recent advances have trended in the direction of selective nanosensors for use in molecular biology DNA whole‐cell profiling 7, 8, there are still considerable efforts that are being placed in the development of higher throughput methodologies that target more comprehensive metabolic profiles of all small biomolecules that are present in the bacterial cells 9–14. Lipidomic analysis by gas chromatography (GC) has an extended history in bacterial taxonomy that dates back to the mid‐1960s, and the current state of the art involves a three‐step procedure (bacterial culturing, lipid derivatization and extraction, GC analysis, and data processing) that is commercially available and used in many applications 15–19.…”

Section: Introductionmentioning

confidence: 99%

The use of GC×GC/TOF MS with multivariate analysis for the characterization of foodborne pathogen bacteria profiles

Gardner¹,

Brillhart²,

Benjamin³

et al. 2010

J of Separation Science

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The cellular fatty acid profiles of eight strains of Bacillus, Staphylococcus, and Enterobacteriacae (Escherichia coli and Salmonella) were analyzed by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry. A novel template method was developed to standardize the raw two-dimensional gas chromatography retention data through the use of a chemical indexing mixture. Analyte retention coordinates were normalized in the primary dimension with respect to a series of n-alkanes (Kovats index) and in the secondary dimension with respect to a series of aromatic hydrocarbons (Lee index). Fatty acid profiles extracted from the templates were compared by multidimensional scaling and principal component analysis. Differences in the profiles of Gram-positive and Gram-negative bacteria were observed, and a series of heterogeneous mixtures comprising different fractions (containing one Gram-positive and one Gram-negative bacteria strain) were also distinguished from their homogeneous constituents.

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“…The ECD is a very sensitive and selective GLC ionization detector. I t is very sensitive to highly halogenated compounds, such as the chlorinated pesticides, and thus has become a standard tool for the detection and estimation of pesticides in trace amounts (in the picogram o'r parts-per-billion range) At the same time there have been a number of reports that biological saniples can be characterized by the GLC patterns or "fingerprints" of their extracts (10)(11)(12)(13). COUpling of the selectivity of the ECD response with the individuality of the GLC profiles enhanced the instrumental characterization of biological samples (14)(15)(16)(17)(18)(19).…”

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confidence: 99%

Characterization of Rat Tissues by Gas-Liquid Chromatography with the Electron-Capture Detector

Theiner

Friedman

1969

Experimental Biology and Medicine

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We examined the feasibility of obtaining gas-liquid chromatographic (GLC) profiles of the distribution of electron-capturing (EC) components in mammalian tissues. The GLC profiles of the unsaponifiable lipids (UL) of rat tissues, monitored simultaneously by a flame ionization detector (FID) and an electron capture detector (ECD), were characteristic of both the source tissue and the individual rat. The GLC profiles of over 100 samples were only superficially similar to each other. Differences in the relative amounts of the EC components created individually characteristic GLC patterns, which were magnified by the removal of cholesterol from the lipid samples. This method provides a means of focusing on the EC lipids, which constitute only several parts per million in the tissue and have molecular weights of several hundred atomic mass units. Some of the EC components observed in the GLC patterns may be the products of pyrolysis of retinol and related compounds.This study was prompted by theoretical (1, 2 ) and experimental (3, 4) demonstrations of the importance of EC compounds in biology, the development of the ECD by Lovelock (S), and the application of the ECD to compounds of biological importance (6)(7)(8). The ECD is a very sensitive and selective GLC ionization detector. I t is very sensitive to highly halogenated compounds, such as the chlorinated pesticides, and thus has become a standard tool for the detection and estimation of pesticides in trace amounts

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Dual-Chamber Micro Cross-Section Detector for Permanent Gas Analysis.

Cited by 7 publications

References 10 publications

Gas Analysis

Gas Analysis

The use of GC×GC/TOF MS with multivariate analysis for the characterization of foodborne pathogen bacteria profiles

Characterization of Rat Tissues by Gas-Liquid Chromatography with the Electron-Capture Detector

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