Dual channel sensor for detection and discrimination of heavy metal ions based on colorimetric and fluorescence response of the AuNPs-DNA conjugates

Tan, Lulu; Chen, Zhengbo; Zhao, Yan; Wei, Xiangcong; Li, Yonghui; Zhang, Chi; Wei, Xinling; Hu, Xiaochen

doi:10.1016/j.bios.2016.05.038

Cited by 117 publications

(40 citation statements)

References 34 publications

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“…Aptamer FRET sensors, or aptasensors, for instance, comprise a class of versatile and very sensitive biosensors, capable of detecting concentrations in nano and picoscales. Ultrasensitive FRET aptasensors for trace detection of metal ions [21,22] and antibiotics [23] are reported. In a multiplexed detection system for Pb(II), Hg(2), and Ag(II) ions, the binding of an ion or multiple ions to DNA sequences triggers the DNA self-assembly.…”

Section: Fret Applied To Environmental Biosensingmentioning

confidence: 99%

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Souza¹,

Martins²,

Colmati³

2019

Biosensors for Environmental Monitoring

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This chapter summarizes the fundamentals of biosensing techniques based on fluorescence spectroscopy and the protagonism of state-of-the-art luminescent biosensors in a wide range of scientific areas, from environmental monitoring to diagnostics and decease treatment, focusing on the paramount contribution of biosensors based on the Förster Resonance Energy Transfer (FRET) transducing mechanism. State-of-the-art FRET biosensors are specially characterized by outstanding sensitivity toward a number of environmental pollutants and dissolved oxygen in aquatic ecosystems, capable of detecting concentrations in the nano and picomolar scales. These biosensors have also been showing impressive performance over other methods in the study of real-time biological processes in vivo relevant to help understanding decease progression like cancer.

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Section: Fret Applied To Environmental Biosensingmentioning

confidence: 99%

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Souza¹,

Martins²,

Colmati³

2019

Biosensors for Environmental Monitoring

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“…Its poisoning of Minamata Bay of Japan widely activated a global attention to mercury (Hg 2+ ) toxicity and its potential consequences to the aquatic ecosystem and human health [ 123 ]. Currently, many types of aptasensors were developed for detecting the Hg 2+ in water samples based on the different platforms, including electrochemistry, colorimetric and fluorescence-based analysis [ 73 , 74 , 75 ]. In addition, many signal amplification modes or novel platforms such as chemiluminescence (CL) [ 73 ], electrically rGO-based chemiresistor [ 69 ], silicon nanomaterials (SiNPs)-based fluorescence [ 70 ], fluorescence lateral flow strip [ 40 ] and exothermic chip [ 71 ] have also been developed for detecting the Hg 2+ in water samples.…”

Section: Aptasensors and Their Applications For Detecting Low Molementioning

confidence: 99%

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

et al. 2018

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Water pollution has become one of the leading causes of human health problems. Low molecular weight pollutants, even at trace concentrations in water sources, have aroused global attention due to their toxicity after long-time exposure. There is an increased demand for appropriate methods to detect these pollutants in aquatic systems. Aptamers, single-stranded DNA or RNA, have high affinity and specificity to each of their target molecule, similar to antigen-antibody interaction. Aptamers can be selected using a method called Systematic Evolution of Ligands by EXponential enrichment (SELEX). Recent years we have witnessed great progress in developing aptamer selection and aptamer-based sensors for low molecular weight pollutants in water sources, such as tap water, seawater, lake water, river water, as well as wastewater and its effluents. This review provides an overview of aptamer-based methods as a novel approach for detecting low molecular weight pollutants in water sources.

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“…Thus, fluorophore-conjugated ssDNAs are capable of generating differential optical signals following interactions with different proteins. There are a number of sensing systems that employ complexes of ssDNAs and other materials, such as gold nanoparticles [ 29 , 30 , 31 ], metal oxide nanoparticles [ 32 , 33 ], graphene oxide nanosheets [ 34 , 35 , 36 , 37 , 38 ], and metal oxide nanosheets [ 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

Okada

Sugai

Tomita

et al. 2020

Sensors

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Recently, pattern-recognition-based protein sensing has received considerable attention because it offers unique opportunities that complement more conventional antibody-based detection methods. Here, we report a multichannel pattern-recognition-based sensor using a set of fluorophore-conjugated single-stranded DNAs (ssDNAs), which can detect various proteins. Three different fluorophore-conjugated ssDNAs were placed into a single microplate well together with a target protein, and the generated optical response pattern that corresponds to each environment-sensitive fluorophore was read via multiple detection channels. Multivariate analysis of the resulting optical response patterns allowed an accurate detection of eight different proteases, indicating that fluorescence signal acquisition from a single compartment containing a mixture of ssDNAs is an effective strategy for the characterization of the target proteins. Additionally, the sensor could identify proteins, which are potential targets for disease diagnosis, in a protease and inhibitor mixture of different composition ratios. As our sensor benefits from simple construction and measurement procedures, and uses accessible materials, it offers a rapid and simple platform for the detection of proteins.

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Dual channel sensor for detection and discrimination of heavy metal ions based on colorimetric and fluorescence response of the AuNPs-DNA conjugates

Cited by 117 publications

References 34 publications

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Dynamic Luminescent Biosensors Based on Peptides for Oxygen Determination

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set

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