Dual-clamped one-pot SERS-based biosensors for rapid and sensitive detection of SARS-CoV-2 using portable Raman spectrometer

Kaladharan, Kiran; Chen, Kuan‐Hung; Chen, P.-H.; Goudar, Venkanagouda S.; Ishdorj, Tseren-Onolt; Santra, Tuhin Subhra; Tseng, Fan‐Gang

doi:10.1016/j.snb.2023.134172

Cited by 9 publications

(2 citation statements)

References 71 publications

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“…The biosensor had a linear range between 100 μg/mL and 0.01 ng/mL with an LOD of 0.046 ng/mL. Another SERS biosensor was developed by Kaladharan et al [ 44 ]. In the preparation of SERS substrates, nanostructures were first formed on digital versatile discs (DVD discs) via the physical vapor deposition method.…”

Section: Introductionmentioning

confidence: 99%

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

Tekin,

Kul,

Sagdic

et al. 2024

Microchim Acta

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The COVID-19 pandemic underlines the need for effective strategies for controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents the potential of nanomaterial-enabled optical biosensors for rapid and low-cost detection of SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, and surface plasmon resonance detection methods. Nanomaterials including metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods, nanoporous materials, nanoshell materials, and magnetic nanoparticles employed in the production of optical biosensors are presented in detail. This review also discusses the detection principles, fabrication methods, nanomaterial synthesis, and their applications for the detection of SARS-CoV-2 in four categories: antibody-based, antigen-based, nucleic acid–based, and aptamer-based biosensors. This critical review includes reports published in the literature between the years 2021 and 2024. In addition, the review offers critical insights into optical nanobiosensors for the diagnosis of COVID-19. The integration of artificial intelligence and machine learning technologies with optical nanomaterial-enabled biosensors is proposed to improve the efficiency of optical diagnostic systems for future pandemic scenarios. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

Tekin,

Kul,

Sagdic

et al. 2024

Microchim Acta

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“…(b) schematic illustration of SERS assay of DENV gene via a cascade enzymefree signal amplification strategy of LCHA and HCR [84] 0907006 -15 (a) Schematic illustration showing the integration of a microfluidic device with the SERSactive substrate based on Au -Ag coated GaN surface [92] ; (b) schematic diagram of CRISPR/Cas12a -SERS principle analysis [93] ; (c) the principle of MPXV detection by colorimetric -SERS dualmode ICA [97] 0907006 -16 using the CRISPR/dCas9 -based SERS method, assisted with HRP -Catalyzed signal amplification [111] ; (b) schematic diagram of the proposed CRISPR/Cas12a -SERS platform for amplificationfree ASFV dsDNA detection [112] 0907006 -18 immunoassay platform(with a portable Raman spectrometer) [115] ; (b) schematic diagram of the detection of influenza A virus H1N1 by SERS and gold electrodeposition nanostructure [116] ; (c) schematic diagram of a SERS detection method based on onestep aptamer identification using a portable Raman spectrometer for rapid and pointofcare detection of SARS -CoV -2 virus in less than 5 minutes [51] 特邀综述…”

mentioning

confidence: 99%

表面增强拉曼光谱技术在流行性病毒检测领域的研究进展

Liu Yi,

Wang Nan,

He Shaohua

et al. 2024

中国激光

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Significance Viruses are the primary cause of many infectious diseases, including influenza, highmortality lower respiratory tract infections, diarrhea, tuberculosis, HIV infection, dengue fever, hepatitis B, and more. These diseases can cause severe damage to various systems in the human body and can even lead to lifethreatening conditions. The outbreak of infectious viruses poses a significant challenge to public healthcare systems. Early and accurate virus diagnosis is crucial in preventing virus spread, especially in the absence of specific vaccines or effective medications. Existing traditional detection methods often require complex equipment and the expertise of skilled operator. Hence, it becomes challenging to conduct largescale testing in rapidly spreading virusinfected areas.Surfaceenhanced Raman scattering (SERS) technology is an ultrasensitive vibrational spectroscopy technique, which is used to detect plasmonic on the surface or nearsurface molecules. Due to its fast response, strong specificity, and noninvasive detection characteristics, SERS has been widely used in surface and interface studies, chemical and biosensors, biomedical monitoring, trace analysis, electrochemical reactions, and catalytic reactions. Specifically, in virus detection, it exhibits extremely high detection sensitivity, enabling rapid and accurate detection of minute virus particles. Based on the analysis of virus spectral features, SERS technology can differentiate between different types of viruses, including subtypes and variants. This high specificity leads to a unique advantage in virus tracing, classification, and epidemiological research, which is crucial for the rapid screening of early virus infections and facilitating timely medical intervention. This review systematically summarizes the research progress and 特邀综述第 51 卷第 9 期/2024 年 5 月/中国激光 viruses. Furthermore, investigating the integration of SERS technology with other detection methods-such as chemical separation, biological capture, colorimetry, and advanced computational approaches like machine learning, deep learning, and artificial intelligence-can maximize the benefits of diverse technologies. This integration promises the creation of innovative Raman analysis devices that consolidate sample processing, detection, analytical processing, statistical analysis, result dissemination, and display functionalities, catering to the onsite and realtime testing demands across various sectors. We expect that merging SERS technology with compact Raman instruments will usher in a convenient, efficient, and precise optical POCT method for virus screening, classification, infection tracking, and prognosis forecasting.

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Research progress and application of two-dimensional materials for surface-enhanced Raman scattering

Zhang,

Peng,

Lin

et al. 2024

Surf. Sci. Tech.

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Surface Enhanced Raman Scattering (SERS) is a pivotal detection technology that identifies molecular spectral signals at the single-molecule level. Owing to its sensitivity and non-destructive nature, SERS has found extensive applications in diverse fields such as environmental science, medical diagnosis, food safety, and bioanalysis. In recent years, two-dimensional materials have emerged as one prominent novel SERS-active nanomaterials due to their unique physical and chemical properties. This paper provides a comprehensive review of the enhancement mechanism of SERS, substrate preparation, and its applications in biosensing, environmental detection, and food safety. It delves into the widely accepted basic theory and enhancement mechanism of SERS, along with common methods to boost SERS performance, including doping with other elements, applying stress and strain, forming defects, and amorphous processing. This paper examines the current state of research on two-dimensional(2D) materials and their application in the field of SERS. Additionally, it provides an viewpoints on recent advancements in the use of this technology for virus and cancer detection. Lastly, it discusses the future prospects for the development of SERS technology.

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Dual-clamped one-pot SERS-based biosensors for rapid and sensitive detection of SARS-CoV-2 using portable Raman spectrometer

Cited by 9 publications

References 71 publications

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era

表面增强拉曼光谱技术在流行性病毒检测领域的研究进展

Research progress and application of two-dimensional materials for surface-enhanced Raman scattering

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