Dual Conjugate Adaptive Optics Prototype for Wide Field High Resolution Retinal Imaging

Popovic, Zoran; Thaung, Jörgen; Knutsson, Per; Owner-Petersen, Mette

doi:10.5772/53640

Cited by 4 publications

(5 citation statements)

References 41 publications

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“…Each of the several hardware and software innovations in this work, individually or together, may be useful in a range of other applications. Our probe and computational algorithms directly or with minor alterations are expected to be useful in a variety of applications for optical engineers, vision scientists, and clinicians for the study of ophthalmic disorders in adults and children, such as for the assessment of progression of hereditary retinal diseases before and after interventions such as gene therapy [59]. Although our intensity-based SZGD algorithm has worked well in the eyes of young children and healthy young adults, it has not been quantitatively evaluated in diseased eyes or the eyes of older subjects.…”

Section: Discussionmentioning

confidence: 99%

Handheld adaptive optics scanning laser ophthalmoscope

DuBose

Nankivil

Larocca

et al. 2018

Optica

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Adaptive optics scanning laser ophthalmoscopy (AOSLO) has enabled in vivo visualization and enhanced understanding of retinal structure and function. Current generation AOSLOs have a large footprint and are mainly limited to imaging cooperative adult subjects. To extend the application of AOSLO to new patient populations, we have designed the first portable handheld AOSLO (HAOSLO) system. By incorporating a novel computational wavefront sensorless AO algorithm and custom optics, we have miniaturized our HAOSLO to weigh less than 200 grams. HAOSLO imaged the cones closest to the fovea with a handheld probe in adults and captured the first AO-enhanced image of cones in infants.

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Section: Discussionmentioning

confidence: 99%

Handheld adaptive optics scanning laser ophthalmoscope

DuBose

Nankivil

Larocca

et al. 2018

Optica

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“…16 It has also been demonstrated in ophthalmoscopy although it has not seen wide-spread adoption. 17,18,19 2. Have each corrector use an independent field of view that can be optically steered to the desired region of interest.…”

Section: Design Considerationsmentioning

confidence: 99%

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

Teverovsky,

Murphy,

Parkins

et al. 2024

Ophthalmic Technologies XXXIV

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Retinal ganglion cells (RGCs) are the layer of neurons in the retina that output directly to the brain. In primates, there are approximately 20 distinct types which each transmit an independent encoding of the external visual world in parallel to the brain. 1 The wide variety of RGC types suggests that the retina undertakes a sophisticated set of computations that our used by higher brain areas to decode the visual world. Yet, little is known about the majority of the different types of RGCs present in the primate retina largely due to the rarity of many of them. Current understanding of RGCs largely derives from ex vivo electrophysiology experiments which are acute and require severing the optic nerve. 2 This preparation removes the opportunity to study vision as an intact system and puts a time limit on how long a sample is viable for study before the tissue dies. Traditional electrophysiology also struggles to study the RGCs that serve the fragile fovea, the central high-resolution region of the retina. Adaptive optics (AO) ophthalmoscopy coupled with functional imaging and optogenetics provides a unique set of tools which allows the study of individual RGCs in situ. 3,4 Here we describe a plan for a novel split field of view AO ophthalmoscope that will deliver visual stimuli precisely controlled in space, time, and color to the receptive fields of specific RGCs, while also simultaneously optically recording functional calcium responses from the same RGCs. The device also enables psychophysical experiments to study the perceptual impact of RGC types via optogenetics.

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“…The problem of limited isoplanatic correction has been addressed with different techniques. Laslandes et al [14], and Popovic et al [15] apply multi-conjugate adaptive optics, using two deformable mirrors. Other approaches, such as anisoplanatic correction with liquid crystals, promise to be effective [16].…”

Section: Introductionmentioning

confidence: 99%

Aberration measurement and correction on a large field of view in fluorescence microscopy

Furieri

Ancora

Calisesi

et al. 2021

Biomed. Opt. Express

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The aberrations induced by the sample and/or by the sample holder limit the resolution of optical microscopes. Wavefront correction can be achieved using a deformable mirror with wavefront sensorless optimization algorithms but, despite the complexity of these systems, the level of correction is often limited to a small area in the field of view of the microscope.In this work, we present a plug and play module for aberration measurement and correction. The wavefront correction is performed through direct wavefront reconstruction using the spinning-pupil aberration measurement and controlling a deformable lens in closed loop. The lens corrects the aberrations in the center of the field of view, leaving residual aberrations at the margins, that are removed by anisoplanatic deconvolution. We present experimental results obtained in fluorescence microscopy, with a wide field and a light sheet fluorescence microscope. These results indicate that detection and correction over the full field of view can be achieved with a compact transmissive module placed in the detection path of the fluorescence microscope.

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Dual Conjugate Adaptive Optics Prototype for Wide Field High Resolution Retinal Imaging

Cited by 4 publications

References 41 publications

Handheld adaptive optics scanning laser ophthalmoscope

Handheld adaptive optics scanning laser ophthalmoscope

A dual adaptive optics instrument for testing the role of retinal ganglion cells in vision

Aberration measurement and correction on a large field of view in fluorescence microscopy

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