Dual-cycle immobilization to reuse both enzyme and support by reblossoming enzyme–inorganic hybrid nanoflowers

Abstract

Both enzyme and support can be recycled using dual-cycle immobilization method by reblossoming the enzyme–inorganic hybrid nanoflowers.

Physicochemical Antioxidative and Emulsifying Properties of Soybean Protein Hydrolysates Obtained with Dissimilar Hybrid Nanoflowers

“…The amide groups provide phosphate crystals to bind flower-like structures and help their growth by providing nucleation sites with different geometrical configurations and densities [ 36 ]. These results are consistent with the findings of Yu et al [ 37 ].…”

Efficient synthesis of vitamin A palmitate in nonaqueous medium using self-assembled lipase TLL@apatite hybrid nanoflowers by mimetic biomineralization

“…However, the hNFs along with high thermal stability could tolerate the temperature environment in the reaction and remain its catalytic activity well. Moreover, the highest catalytic efficiency (K cat /K m ) of the hNFs was 38.52 mM −1 s −1 in the enzyme activity assay, 21.7 folds than that of free enzyme (27). Moreover, the strong interaction of much Ca 2+ ions in the hNFs with the carboxyl groups of substrates would enrich the substrate in the inner compact layer and near diffusion layer of the hNFs and further improve the reaction rate and the yield of product.…”

“…Even if reaction time went towards, the yield was not found to change clearly. In addition, the yields of product using the hNFs were always higher than that using free TLL, which may be resulted from the lower thermal stability of free TLL than the hNFs (27). Free TLL would lose its most activity within the long period of reaction time and could not catalyze the transesterification of vitamin A acetate and palmitic acid any longer.…”

“…Ke et al (26) have described a new hybrid nanoflower using the organic component of Burkholderia cepacia lipase and an inorganic component of calcium phosphate, and the activity was 308% folds of the free one. In addition, in our previous work, we have also developed a sustainable dual-cycle method depending on calcium phosphate crystallization, dissolution and recrystallization, to reuse both enzyme and calcium phosphate support by re-blossoming enzyme-inorganic hybrid nanoflowers (27).…”

Putting precision and elegance in enzyme immobilisation with bio-orthogonal chemistry

“…The cost, commercial availability and recyclability of the carrier plays an important role in determining the commercial viability of the method as a whole. [232][233][234] Turner and coworkers 11 provided guidelines for the immobilisation of enzymes for use in the pharmaceutical industry. The immobilised enzyme should have a wt loading of 410%, an activity recovery of 450%, have 420 recycles, a cost contribution of o5%, exhibit no leaching, tolerance to organic solvents, be mechanically stable in batch and in flow and good substrate mass transport.…”