Dual-degradable disulfide-containing PEI&amp;ndash;Pluronic/DNA polyplexes: transfection efficiency and balancing protection and DNA release

Zhang, Lifen; Chen, Zhenzhen; Li, Yangfeng

doi:10.2147/ijn.s49595

Cited by 25 publications

(14 citation statements)

References 37 publications

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“…Lowmolecular-weight PEI, such as PEI-600 Da has low cytotoxicity, but it shows very low transfection efficiency. [15][16][17] In this regard, it has been demonstrated that the size (molecular weight), compactness, and chemical modification of PEI affect the efficacy and toxicity of this polymer. Xia et al used several PEI polymer sizes ranging from molecular weights of 0.6-25 kD in order to balance the efficiency of nucleic acid delivery and cellular toxicity.…”

mentioning

confidence: 99%

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Li¹,

Shen²,

Chen³

et al. 2015

IJN

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Engineering a safe and high-efficiency delivery system for efficient RNA interference is critical for successful gene therapy. In this study, we designed a novel nanocarrier system of polyethyleneimine (PEI)-modified Fe 3 O 4 @SiO 2 , which allows high efficient loading of VEGF small hairpin (sh)RNA to form Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites for VEGF gene silencing as well as magnetic resonance (MR) imaging. The size, morphology, particle stability, magnetic properties, and gene-binding capacity and protection were determined. Low cytotoxicity and hemolyticity against human red blood cells showed the excellent biocompatibility of the multifunctional nanocomposites, and also no significant coagulation was observed. The nanocomposites maintain their superparamagnetic property at room temperature and no appreciable change in magnetism, even after PEI modification. The qualitative and quantitative analysis of cellular internalization into MCF-7 human breast cancer cells by Prussian blue staining and inductively coupled plasma atomic emission spectroscopy analysis, respectively, demonstrated that the Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites could be easily internalized by MCF-7 cells, and they exhibited significant inhibition of VEGF gene expression. Furthermore, the MR cellular images showed that the superparamagnetic iron oxide core of our Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites could also act as a T 2 -weighted contrast agent for cancer MR imaging. Our data highlight multifunctional Fe 3 O 4 @SiO 2 /PEI/VEGF shRNA nanocomposites as a potential platform for simultaneous gene delivery and MR cell imaging, which are promising as theranostic agents for cancer treatment and diagnosis in the future.

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mentioning

confidence: 99%

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Li¹,

Shen²,

Chen³

et al. 2015

IJN

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“…Very few studies are available in the literature in which the release strategy based on a reductively and a hydrolytically dual rupture of DNA polyplexes has been exploited [35,36]. We believe that the study we present in this paper, based on an under-exploited topic such as dualbreakable polymers will be useful for researchers interested in developing new carriers for the delivery of nucleic acids.…”

Section: Introductionmentioning

confidence: 93%

Complexation and release of DNA in polyplexes formed with reducible linear poly(β-amino esters)

Rata-Aguilar

Segovia

Jódar-Reyes

et al. 2015

Colloids and Surfaces B: Biointerfaces

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“…Low molecular weight forms (2 kDa or less) are less toxic but they are less effective as transfection agents. 87 Therefore, PEIs at and below 25 kDa with a branched or linear architecture are commonly used as gene delivery reagents. [88][89][90][91] The golden standard gene delivery polymer is branched PEI of 25 kDa, which offers a balance between the toxicity, nucleic acid binding/protection and release.…”

Section: Peimentioning

confidence: 99%

“…Alternatively, short PEI chains attached together with hydrolysable links such as disulfide and ester, may provide initially a high molecular weight PEI system for good nucleic acid condensation and protection, but upon dissolution because of intracellular redox conditions, they may act as a low molecular weight PEI polymers with lower toxicity. 87 Additionally, pluronics, that are block copolymers of ethylene glycolpropylene glycol-ethylene glycol, can be used instead of PEG. Similar to PEG, pluronics were shown to improve the biocompatibility and bioavailability of the nanocarriers, but they interact better with the cell membrane due to the propylene glycol units and enhance cellular uptake of the particles.…”

Section: Peimentioning

confidence: 99%

Anticancer Use of Nanoparticles as Nucleic Acid Carriers

Gözüaçık¹,

Yağcı-Acar²,

Akkoç³

et al. 2014

j biomed nanotechnol

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Advances in nanotechnology opened up new horizons in the field of cancer research. Nanoparticles made of various organic and inorganic materials and with different optical, magnetic and physical characteristics have the potential to revolutionize the way we diagnose, treat and follow-up cancers. Importantly, designs that might allow tumor-specific targeting and lesser side effects may be produced. Nanoparticles may be tailored to carry conventional chemotherapeutics or new generation organic drugs. Currently, most of the drugs that are commonly used, are small chemical molecules targeting disease-related enzymes. Recent progress in RNA interference technologies showed that, even proteins that are considered to be "undruggable" by small chemical molecules, might be targeted by small RNAs for the purpose of curing diseases, including cancer. In fact, small RNAs such as siRNAs, shRNAs and miRNAs can drastically change cellular levels of almost any given disease-associated protein or protein group, resulting in a therapeutic effect. Gene therapy attempts were failing mainly due to delivery viral vector-related side effects. Biocompatible, non-toxic and efficient nanoparticle carriers raise new hopes for the gene therapy of cancer. In this review article, we discuss new advances in nucleic acid and especially RNA carrier nanoparticles, and summarize recent progress about their use in cancer therapy.

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Dual-degradable disulfide-containing PEI–Pluronic/DNA polyplexes: transfection efficiency and balancing protection and DNA release

Cited by 25 publications

References 37 publications

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Polyetherimide-grafted Fe3O4@SiO2 nanoparticles as theranostic agents for simultaneous VEGF siRNA delivery and magnetic resonance cell imaging

Complexation and release of DNA in polyplexes formed with reducible linear poly(β-amino esters)

Anticancer Use of Nanoparticles as Nucleic Acid Carriers

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