Dual detection of nafcillin using a molecularly imprinted polymer-based platform coupled to thermal and fluorescence read-out

Hudson, Alexander; Jamieson, Oliver; Crapnell, Robert D.; Rurack, Knut; Soares, Thais C. C.; Mecozzi, Francesco; Laude, Alex; Gruber, Jonas; Novakovic, Katarina; Peeters, Marloes

doi:10.1039/d1ma00192b

Cited by 15 publications

(7 citation statements)

References 43 publications

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“…Producing a low-cost sensor platform, using a direct polymerisation technique, the sensor was able to achieve a limit of detection (LOD) of 0.54 nM (23). By incorporating a fluorescent monomer (fluorescein methacrylate) into a MIP film allowed Hudson et al to produce a sensor that was capable of detecting nafcillin, offering the potential for a portable, on-site sensor (24). MIPs have shown demonstrable application in solid phase extraction protocols and a range of fluoroquinolones have been targeted from environmental and animal product samples (25)(26)(27).…”

Section: Introductionmentioning

confidence: 99%

A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk

et al. 2022

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Using a solid-phase molecular imprinting technique, high affinity nanoparticles (nanoMIPs) selective for the target antibiotics, ciprofloxacin, moxifloxacin and ofloxacin have been synthesised. These have been applied in the development of a surface plasmon resonance (SPR) sensor for the detection of the three antibiotics in both river water and milk. The particles produced demonstrated good uniformity with approximate sizes of 65.8 ± 1.8 nm, 76.3 ± 4.1 nm and 85.7 ± 2.5 nm, and were demonstrated to have affinities of 36.2 nM, 54.7 nM and 34.6 nM for the ciprofloxacin, moxifloxacin and ofloxacin nanoMIPs, respectively. Cross reactivity studies highlighted good selectivity towards the target antibiotic compared with a non-target antibiotic. Using spiked milk and river water samples the nanoMIP-based SPR sensor offered comparable affinity with 66.8 nM, 33.4 nM and 55.0 nM (milk) and 39.3 nM, 26.1 nM and 42.7 nM (river water) for ciprofloxacin, moxifloxacin and ofloxacin nanoMIPs respectively, to that seen within a buffer standard. Estimated LOD's for the three antibiotic targets in both milk and river water were low nM or below. The developed SPR sensor showed good potential for using the technology for the capture and detection of antibiotics from food and environmental samples.

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Section: Introductionmentioning

confidence: 99%

A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk

et al. 2022

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“…[29][30][31][32][33] Moreover, the MIP-based fluorescent sensor was designed to achieve nM level sensitivity, like MIP-based screen printed electrodes for penicillin detection via measuring the thermal resistance difference at the solid-liquid interface, MIP-based 3D-printed flow cells for nafcillin detection using a combined thermal and fluorescence device, etc. 34,35 First, HNTs were used as a building block, and were then combined with Fe 3 O 4 nanoparticles through their surface hydroxyl groups, so as to speed up the detection time and facilitate the extraction. Afterwards, a fluorescent MIP layer was synthesized onto the MHNTs, using methacrylic acid (MAA) and allyl rhodamine B (ARhB, a polymerizable fluorophore) as functional monomers to exhibit hydrogen bonding and boron affinity toward OTC, respectively.…”

Section: Introductionmentioning

confidence: 99%

A molecularly imprinted antibiotic receptor on magnetic nanotubes for the detection and removal of environmental oxytetracycline

Wang

Zhang

et al. 2022

J. Mater. Chem. B

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“…[182] Since the polymer film can be designed with several monomers and/or cross-linker molecules, the variation of one of mixture components can have a huge impact not only on the sensitivity of the sensor but also on the transducer used to convert a given signal. For this reason, these films have found application with several readout technologies such as: HTM, [76,97,183] EIS, [76] CV, [184,185] DPV, [186][187][188][189] QCM, [70,[190][191][192] GC-MS, [193,194] SPR, [195,196] APGC-MS/MS, [197] UHPLC-PDA, [198] UV-Vis. [199] Figure 8.…”

Section: Thin Filmsmentioning

confidence: 99%

Deposition Methods for the Integration of Molecularly Imprinted Polymers (MIPs) in Sensor Applications

Caldara

Wissen

Cleij

et al. 2023

Advanced Sensor Research

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Offering high specificity and selectivity, molecularly imprinted polymers (MIPs) are synthetic polymeric affinity reagents that have become increasingly popular over the last couple of decades. Due to their long-term chemical and physical stability and low production cost, they have become an increasingly popular choice of receptor in the realm of sense. MIPs have therefore been associated with the detection of small molecules, proteins, cells, and pathogens, proving a highly robust and useful tool in the production of next-gen sensing platforms. This said, the development of these sensors pivots on one simple fact; these receptors have to be deposited onto a substrate for their desired application. The deposition of MIPs during sensor fabrication is therefore of great importance, with the field utilizing an array of mechanical and chemical deposition methods to achieve this. To this end, this review, therefore, sets aim at coalescing these different deposition approaches, classifying them, and outlining their utility when it comes to receptor design and integration. Thus, offering a knowledge base on current deposition methods, potential future approaches and analyzing where the MIP deposition field is tending toward.

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Dual detection of nafcillin using a molecularly imprinted polymer-based platform coupled to thermal and fluorescence read-out

Abstract: Reported here is the production of molecularly imprinted polymer (MIP) films, integrating a fluorescent moiety that serves as both, an element for template interaction and signalling, for the thermal and...

Cited by 15 publications

References 43 publications

A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk

A molecularly imprinted polymer nanoparticle-based surface plasmon resonance sensor platform for antibiotic detection in river water and milk

A molecularly imprinted antibiotic receptor on magnetic nanotubes for the detection and removal of environmental oxytetracycline

Deposition Methods for the Integration of Molecularly Imprinted Polymers (MIPs) in Sensor Applications

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