Dual Detection of Procalcitonin and C-reactive Protein with an Up-converting Nanoparticle Based Lateral Flow Assay

Abstract

Procalcitonin (PCT) and C-reactive protein (CRP) are significant complementary inflammatory markers, and their simultaneous detection is of substantial value. In this article, a rapid, simple and cost-effective method for the dual quantitative detection of PCT and CRP in serum is discussed. Two UCNPs of similar size and morphology, but with a different emission spectrum, were prepared for the simultaneous detection of PCT and CRP by lateral flow assay (LFA) technology in a direct method. With the developed dual test strips and signal read system, the assay results present a limit of detection (LOD) of 0.12 ng/mL and 0.24 μg/mL for PCT and CRP, respectively. In addition, both of the coefficients of variation and positive and negative concordance rates for PCT or CRP are well compared with those of the traditional method. The dual detection of PCT and CRP have shown great application potential in medical diagnosis and treatment guidance.

Compact Magneto‐Fluorescent Colloids by Hierarchical Assembly of Dual‐Components in Radial Channels for Sensitive Point‐of‐Care Immunoassay

“…Therefore, the SIQS‐based LFIA provides flexible dual‐modal signal readouts for personalized quantification scenarios and the potential for self‐validating to exclude background interferences. [ 62–65 ] Compared with previous reports for PCT detection, the SIQS‐based LFIA exhibits high sensitivity and relatively short detection time (Table S2, Supporting Information), [ 66–73 ] demonstrating the potential of the proposed assay for POC detecting of PCT.…”

Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples

“…1,2 CRP is oen used for early diagnosis of inammatory and infectious diseases, as many of these diseases are mainly caused by the increasing CRP level in blood. [3][4][5][6] Generally, the normal concentration of CRP in blood is below 5 mg mL À1 , and it could increase to 10-40 mg mL À1 during mild inammation or viral infection in patients. 7 Recently, many researchers reported that higher values of high-sensitivity CRP (hs-CRP, 0.1-3.0 mg mL À1 ) are highly related to cardiovascular diseases.…”

“…The lateral ow immunoassay (LFIA) is a immunochromatography technology, which is based on specic conjugation of antigen-antibody and chromatography. 4,15,16 Aer dropping the sample solution, the labeled probes will rapidly and specically conjugate with the target protein, and then, they are captured by antibodies on the test line (T line) and the unconjugated probes are captured by secondary antibodies on the control line (C line) under capillary action. 17 Paper-based lateral ow immunochromatographic strips with the advantages of rapid detection, simple operation and low cost have been widely used for POCT in food, environmental and clinical elds.…”

Detection in Immunoassays

“…For example, up-converting nanoparticles were labeled with antibodies and used for immunochromatography. 5 In addition, it was also suggested that the sensitivity of conventional fluorescence immunoassays may be increased by using a silver nanoparticle-immobilized well plate due to its fluorescence enhancement effect. 6 Some nanomaterials are used as nanocarriers that can release large amounts of signal reporters.…”