Dual detection system for cancer-associated point mutations assisted by a multiplexed LNA-based amperometric bioplatform coupled with rolling circle amplification

Sebuyoya, Ravery; Valverde, Alejandro; Moranova, Ludmila; Strmiskova, Johana; Hrstka, Roman; Montiel, Víctor Ruiz-Valdepeñas; Pingarrón, José M.; Barderas, Rodrigo; Campuzano, Susana; Bartosik, Martin

doi:10.1016/j.snb.2023.134375

Cited by 5 publications

(1 citation statement)

References 43 publications

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“…For DNA amplification, isothermal techniques have recently gathered much attention from researchers owing to their rapidity, cost effectiveness, ease of use, and ability to be performed under constant temperature. Some common isothermal techniques include loop-mediated isothermal amplification (LAMP) [13][14][15], rolling circle amplification (RCA) [16,17], nucleic acid sequence-based amplification (NASBA) [18,19], and recombinase polymerase amplification (RPA) [20,21]. Among these listed isothermal techniques, LAMP serves as the most promising approach because it requires less enzyme compared to RPA.…”

Section: Introductionmentioning

confidence: 99%

Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens

Nguyen,

Lee

2023

Sensors

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A pipette-free and fully integrated device that can be used to accurately recognize the presence of infectious pathogens is an important and useful tool in point-of-care testing, particularly when aiming to decrease the unpredictable threats posed by disease outbreak. In this study, a paper device is developed to integrate the three main processes required for detecting infectious pathogens, including DNA extraction, loop-mediated isothermal amplification (LAMP), and detection. All key reagents, including sodium dodecyl sulfate (SDS), NaOH, LAMP reagents, and carmoisine, are placed on the paper device. The paper device is operated simply via sliding and folding without using any bulky equipment, and the results can be directly observed by the naked eye. The optimized concentrations of sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), and carmoisine were found to be 0.1%, 0.1 M, and 0.5 mg/mL, respectively. The paper device was used to detect Enterococcus faecium at concentrations as low as 102 CFU/mL within 60 min. Also, E. faecium spiked in milk was successfully detected using the paper device, demonstrating the feasible application in real sample analysis.

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Section: Introductionmentioning

confidence: 99%

Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens

Nguyen,

Lee

2023

Sensors

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Electrochemical Detection of BRAF V600E Mutation in a Liquid Biopsy Format: LAMPing the Way to an Improved Diagnostics and Treatment

Moranova,

Strmiskova,

Ondraskova

et al. 2024

Adv Materials Technologies

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The BRAF V600E substitution, predominantly found in melanoma and metastatic colorectal cancer (mCRC), is associated with aggressive traits and poor prognosis. Molecular testing of the BRAF mutational status is crucial for guiding therapeutic decisions, driving the necessity for new rapid diagnostic technologies. Here, a novel LAMP‐based assay is presented for fast and selective amplification of mutated DNA, coupled with simple electrochemical detection on electrode biochips. The assay demonstrated high selectivity across cell lines and in 51 cancer patients, detecting mutations in both tumor tissue DNA and circulating tumor DNA (ctDNA) in serum. Clear discrimination between the mutated and wild‐type sequences is achieved, in perfect agreement with either NGS or ddPCR. The bioassay offers a simple and efficient alternative to traditional PCR methods for point‐of‐care diagnostics, particularly in settings with limited equipment and resources.

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A novel RCA-based DNA sensor system for specific and quantitative detection of Klebsiella pneumonia

Chen,

Lu,

Din

et al. 2024

Microchemical Journal

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Dual detection system for cancer-associated point mutations assisted by a multiplexed LNA-based amperometric bioplatform coupled with rolling circle amplification

Cited by 5 publications

References 43 publications

Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens

Pipette-Free and Fully Integrated Paper Device Employing DNA Extraction, Isothermal Amplification, and Carmoisine-Based Colorimetric Detection for Determining Infectious Pathogens

Electrochemical Detection of BRAF V600E Mutation in a Liquid Biopsy Format: LAMPing the Way to an Improved Diagnostics and Treatment

A novel RCA-based DNA sensor system for specific and quantitative detection of Klebsiella pneumonia

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