Dual drug release mechanisms through mesoporous silica nanoparticle/electrospun nanofiber for enhanced anticancer efficiency of curcumin

Abstract

Electrospun nanofibers (NFs)‐based drug delivery approaches are of particular interest as a hopeful implantable nanoplatform for localized cancer therapy and treating tissue defect after resection, allowing the on‐site drug delivery with minimal side effect to healthy cells. To maintain therapeutic concentrations of anticancer molecules for a relatively long time through a combination of burst and sustained drug release mechanisms, a hybrid of polycaprolactone and gelatin (PCL/GEL) was used for co‐encapsulation of free curcumin (CUR) and CUR‐loaded mesoporous silica nanoparticles (CUR@MSNs) via electrospinning, resulting in a novel drug‐loaded nanofibrous scaffold, CUR/CUR@MSNs‐NFs. The as‐prepared MSNs and composite NFs were characterized via TGA, FTIR, FE‐SEM, TEM, and BET. In vitro release profile of CUR from CUR/CUR@MSNs‐NFs was examined, and the in vitro antitumor efficacy against MDA‐MB‐231 breast cancer cells was also evaluated through MTT, scratch assay, DAPI staining, and real‐time PCR. The results disclosed that the smooth, bead‐free, and randomly oriented CUR/CUR@MSNs‐NFs displayed a combination of initial rapid discharge and sustained release for CUR, which led to higher cytotoxicity, lower migration as well as a more pronounced effect on apoptosis induction than CUR‐NFs and CUR@MSNs‐NFs. The present study illustrated that the dual drug release mechanisms through MSN/NF‐mediated drug delivery systems might have a highly hopeful application as a localized implantable scaffold for potential postoperative breast cancer therapy.

Preparation, Characterization, and In Vitro Release of Curcumin-Loaded IRMOF-10 Nanoparticles and Investigation of Their Pro-Apoptotic Effects on Human Hepatoma HepG2 Cells

“…Compared to the solvothermal method, this method can be performed at room temperature, which is safer and more convenient, and can rapidly synthesize MOFs in a short time [4]. To date, many materials have been used as carrier materials for curcumin, such as CUR-loaded solid lipid nanoparticles (37% ± 2.5%) [41], CUR-loaded chitosan nanoparticles (18%) [42], CUR-loaded mesoporous silica nanoparticles (MSNs), and CUR-loaded amine-functionalized MSNs (7.9%, 24.4%) [43]. The common drug-loading methods for MOFs include the solvent adsorption method [44], covalent crosslinking method [45], and an insitu growth method [46].…”

Liposome-based nanocapsules for the controlled release of dietary curcumin: PDDA and silica nanoparticle-coated DMPC liposomes enhance the fluorescence efficiency and anticancer activity of curcumin

“…In this study the UV-visible spectrophotometer technique was applied to monitor curcumin's release. 37,38 The release of curcumin from the nanocapsules was recorded by measuring the absorbance of curcumin at different time intervals. Curcumin's concentration was calculated based on the Beer-Lambert law.…”

Electrospun Composite Nanofibers for Functional Applications

“…Recently, Xu et al prepared CUR/CUR@MSNs-NFs by ESP by loading curcumin (CUR) in SiO 2 NPs. The scaffolds exhibited excellent biphasic release, which released 54% in the first 3 days and all in 35 days [118]. In addition, by modifying the SiO 2 NPs, the amine-functionalized MSNs are hydrophilic and positively charged, resulting in a more prominent anti-cancer effect [119].…”