Dual Drugs Anticancer Nanoformulation using Graphene Oxide-PEG as Nanocarrier for Protocatechuic Acid and Chlorogenic Acid

Saifullah, Bullo; Buskaran, Kalaivani; Baby, Rabia; Dorniani, Dena; Fakurazi, Sharida; Hussein, Mohd Zobir

doi:10.1007/s11095-019-2621-8

Cited by 48 publications

(32 citation statements)

References 62 publications

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“…This composition is a reason for the aforementioned hydrophilicity, the good aqueous dispersity, and the excellent ability to easily cross cell membranes. The functional groups also allow for the capture, anchoring, or immobilization of polymers or biomolecules, such as nucleic acids, as well as drugs, NPs, etc., [24] and, for that reason, GO is a suitable carrier for delivering proteins, peptides, RNA, DNA, and various molecules of biologically active substances with antiviral, antibacterial, or anticancer activities into cells [25]. For applications in the biomedical area, especially for delivering systems or antimicrobial materials, GO is usually synthetized from graphite using various modifications of Hummers' method.…”

Section: Graphene-based Materialsmentioning

confidence: 99%

“…The covalent interactions of the carboxyl groups of GO with the amine group of peptides or polymer surface of rGO lead to a decrease of the content of oxygen, and, on the other hand, GO conductivity was increased in the biological fluid. Thus, modification techniques such as the application of PEG enhance the dispersity and solubility of GO, even when in salt or serum for a long time [24].…”

Section: Drug Delivery Systemsmentioning

confidence: 99%

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Graphenic Materials for Biomedical Applications

2019

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Graphene-based nanomaterials have been intensively studied for their properties, modifications, and application potential. Biomedical applications are one of the main directions of research in this field. This review summarizes the research results which were obtained in the last two years (2017-2019), especially those related to drug/gene/protein delivery systems and materials with antimicrobial properties. Due to the large number of studies in the area of carbon nanomaterials, attention here is focused only on 2D structures, i.e. graphene, graphene oxide, and reduced graphene oxide.

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Section: Graphene-based Materialsmentioning

confidence: 99%

Section: Drug Delivery Systemsmentioning

confidence: 99%

Graphenic Materials for Biomedical Applications

2019

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“…It not only has pharmacological activities, such as anti-platelet agglutination, reducing myocardial oxygen consumption, improving myocardial oxygen tolerance, slowing heart rate, bacteriostasis, and analgesia [29][30][31] , but also has antioxidant activities and tumor and neuroprotective effects 32,33 . For example, some studies have found that protocatechuic acid has anti-in ammatory, bacteriostasis 34,35 , anti-oxidation 36 , and anti-tumor effects 37 , and plays a role in the protection of cerebral hemorrhage 38 .…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Protocatechuic Acid on Platelet Aggregation Induced by High Shear Stress

Dan

et al. 2020

Preprint

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Background Platelet aggregation helps stop bleeding and thrombosis and blocks atherosclerosis, a cause of heart and cerebrovascular disease. Presently, bleeding and drug resistance are the most common side effects of clinically used antiplatelet drugsthus, it is particularly important to identify new antiplatelet drugs that can avoid or reduce these side effects. Method In this study,we used microfluidic chips in vitro to simulate the highly narrow bionic blood vessels of atherosclerosis to investigate the platelet aggregation on shear force-induced drug inhibition. Use flow cytometry to detect platelet activation markers（CD62P）.And thromboelasmograph and automatic coagulation analyzer were used to detect the coagulation function of blood after the action of protocatechuic acid. Results we found that platelets strongly aggregated after passing through the highly narrow microfluidic channel, and the aggregation can be inhibited by protocatechuic acid and CD42b but not by aspirin, tirofiban and other drugs.and protocatechuic acid inhibits platelet aggregation mainly by inhibiting interactions between platelet glycoprotein 1b (GPIb) and von Willebrand factor (vWF)，but does not activate platelets（ did not increase the expression of CD62P, a platelet activation marker）.In addition, protocatechuic acid did not significantly inhibit platelet aggregation induced by other endogenous agonists, such as collagen and ADP.It was found that the inhibitory effect of protocatechuic acid on platelet aggregation did not affect blood production, coagulation time, or coagulation function, reducing the drug resistance and frequent bleeding caused by aspirin. Conclusion Protocatechuic acid could selectively inhibit high shear force-induced platelet aggregation without affecting blood clotting function, However, the effect of the combination of the Protocatechuic acid and aspirin or other drugs remains to be studied. Therefore, we will conduct further studies on protocatechuic acid for new antithrombotic drugs, which can prevent thrombosis without affecting clotting time.

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“…Various researchers have reported PCA to have significant antioxidant, antibacterial, anti-inflammatory, analgesic and antiseptic properties [62,63]. In a study conducted by Bullo et al [64], PCA and chlorogenic acid (CA) were loaded into an anticancer nanocarrier graphene oxide-polyethylene glycol (GOPEG) and tested in phosphate buffer saline to assess the release properties and also tested against HEPG2 (liver cancer), HT-29 cells (human colon cancer) and normal 3T3 cells (fibroblast cells). It was found that PCA and CA were released in a sustained manner, having strong activity against cancerous cells and biocompatibility with normal cells.…”

Section: Discussionmentioning

confidence: 99%

Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential

Sharma

Bhatia

et al. 2019

Antioxidants

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Plants have been the basis of traditional medicine since the dawn of civilizations. Different plant parts possess various phytochemicals, playing important roles in preventing and curing diseases. Scientists, through extensive experimental studies, are playing an important part in establishing the use of phytochemicals in medicine. However, there are still a large number of medicinal plants which need to be studied for their phytochemical profile. In this study, the objective was to isolate phytochemicals from bark of Bauhinia variegata L. and to study them for their antioxidant and cytotoxic activities. The bark was extracted with methanol, followed by column chromatography and thus isolating kaempferol, stigmasterol, protocatechuic acid-methyl ester (PCA-ME) and protocatechuic acid (PCA). 2,2-azinobis-3-ethyl-benzothiazoline-6-sulfonic acid (ABTS) and 2, 2’-diphenyl-1-picrylhydrazyl radical (DPPH) radical scavenging assays were utilized for assessment of antioxidant activity, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye reduction assay was used to determine cytotoxic activity against C-6 glioma rat brain, MCF-7 breast cancer, and HCT-15 colon cancer cell lines. The compounds were found to have significant antioxidant and cytotoxic activity. Since there is a considerable increase in characterizing novel chemical compounds from plant parts, the present study might be helpful for chemotaxonomic determinations, for understanding of medicinal properties as well as for the quality assessment of herbal supplements containing B. variegata bark, thus establishing its use in traditional medicine.

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Dual Drugs Anticancer Nanoformulation using Graphene Oxide-PEG as Nanocarrier for Protocatechuic Acid and Chlorogenic Acid

Cited by 48 publications

References 62 publications

Graphenic Materials for Biomedical Applications

Graphenic Materials for Biomedical Applications

Inhibitory Effect of Protocatechuic Acid on Platelet Aggregation Induced by High Shear Stress

Isolation of Phytochemicals from Bauhinia variegata L. Bark and Their In Vitro Antioxidant and Cytotoxic Potential

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