Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

De-Paula, Mirian Michelle Machado; Afewerki, Samson; Viana, Bartolomeu C.; Webster, Thomas J.; Lobo, Anderson Oliveira; Marciano, Fernanda Roberta

doi:10.1016/j.msec.2019.109778

Cited by 21 publications

(10 citation statements)

References 59 publications

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“…A tubular vascular tissue engineering scaffold comprising PCL/collagen core-shell nanofibers was prepared by using coaxial electrospinning and found to have good biocompatibility and cell affinity [87]. Core-shell electrospun scaffolds with suitable mechanical properties, good hydrophilicity, the ability to promote osteoblast maturation and antibacterial properties were prepared by De-Paula et al [88]. Coimbra et al used PCL and synthetic gel-methacrylate (GelMA) in coaxial electrospinning to prepare a core-shell nanofiber network for vascular tissue regeneration.…”

Section: Cell Scaffoldsmentioning

confidence: 99%

The Development and Bio-applications of Multifluid Electrospinning

Wang¹,

Yu²,

Li³

et al. 2020

MAHIG

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Electrospinning is a potent "top-down" nanofabrication method and has been explored by many researchers in recent decades because it is an efficient, versatile, simple, and low-cost route to prepare nanofibers. Nanofiber membranes generated by electrospinning have been used in various fields including tissue engineering, wound dressing, biosensing, theranostics, and functional textiles. Here we summarize the development of multifluid electrospinning processes, including coaxial, Janus and triaxial electrospinning, and the applications of these techniques. Complex nanostructures prepared by multifluid electrospinning are considered. The key parameters that affect the electrospinning process are introduced, and a discussion of both equipment and solution parameters is presented.

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Section: Cell Scaffoldsmentioning

confidence: 99%

The Development and Bio-applications of Multifluid Electrospinning

Wang¹,

Yu²,

Li³

et al. 2020

MAHIG

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“…Electrospun fibres provide matrices in which active compounds can be incorporated. Fibres with a core-shell structure fabricated via coaxial electrospinning can be loaded with a core of antimicrobial compounds [109,110], as well as corrosion inhibitors and self-healing agents [111]. The shell of the fibres will serve to protect and control the release.…”

Section: Release Of Active Compoundsmentioning

confidence: 99%

Electrospinning: A Powerful Tool to Improve the Corrosion Resistance of Metallic Surfaces Using Nanofibrous Coatings

Rivero

Redin

Rodríguez

2020

Metals

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The use of surface engineering techniques to tune-up the composition of nanostructured thin-films for developing functional coatings with advanced properties is a hot topic within the scientific community. The control of the coating structure at the nanoscale level allows improving the intrinsic properties of the surface compared to bulk materials. A nanodeposition technique with increasing popularity in the field of nanotechnology is electrospinning. This technique permits the fabrication of long and continuous fibres on the micro-nano scale. The good control over fibre morphology combined with its simplicity, cost-effectiveness, easy exploitability and scalability make electrospinning a very interesting tool for technological applications. This review is focused on the use of the electrospinning technique to protect metallic surfaces against corrosion. Polymeric precursors, from natural or biodegradable to synthetic polymers and copolymers can be electrospun with an adequate control of the operational deposition parameters (applied voltage, flow rate, distance tip to collector) and the intrinsic properties of the polymeric precursor (concentration, viscosity, solvent). The electrospun fibres can be used as an efficient alternative to encapsulate corrosion inhibitors of different nature (inorganic or organic) as well as self-healing agents which can be released to reduce the corrosion rate in the metallic surfaces.

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“…One advantage of electrospinning is its versatility in directly incorporating bioactive agents, including anti-cancer drugs, nanoparticles, growth factors, into the nanocellulose matrix without complicated processes (Xue et al, 2014;Ren et al, 2017). However, a specific disadvantage that should be considered while dealing with the drug delivery system is its high initial release that may cause irreversible damage to the surrounding cells or tissues (Khalf and Madihally, 2017;De-Paula et al, 2019;Pant et al, 2019). To address this issue, the coaxial electrospun fiber composed of the shell layer and the core layer was introduced to fabricate the bioactive agents-incorporated cellulose membranes (Dayem et al, 2016;Si et al, 2016;Hickey et al, 2017;Ke et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

Peng

Ren

Zhang

et al. 2021

Front. Bioeng. Biotechnol.

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Electrospinning technique has attracted considerable attention in fabrication of cellulose nanofibrils or nanocellulose membranes, in which polycaprolactone (PCL) could be used as a promising precursor to prepare various cellulose nanofibril membranes for periodontal tissue regeneration. Conventional bio-membranes and cellulose films used in guided tissue regeneration (GTR) can prevent the downgrowth of epithelial cells, fibroblasts, and connective tissue in the area of tooth root but have limitations related to osteogenic and antimicrobial properties. Cellulose nanofibrils can be used as an ideal drug delivery material to encapsulate and carry some drugs. In this study, magnesium oxide (MgO) nanoparticles-incorporated PCL/gelatin core-shell nanocellulose periodontal membranes were fabricated using coaxial electrospinning technique, which was termed as Coaxial-MgO. The membranes using single-nozzle electrospinning technique, namely Blending-MgO and Blending-Blank, were used as control. The morphology and physicochemical property of these nanocellulose membranes were characterized by scanning electron microscopy (SEM), energy-dispersive spectrum of X-ray (EDS), transmission electron microscopy (TEM), contact angle, and thermogravimetric analysis (TGA). The results showed that the incorporation of MgO nanoparticles barely affected the morphology and mechanical property of nanocellulose membranes. Coaxial-MgO with core-shell fiber structure had better hydrophilic property and sustainable release of magnesium ion (Mg2+). CCK-8 cell proliferation and EdU staining demonstrated that Coaxial-MgO membranes showed better human periodontal ligament stem cells (hPDLSCs) proliferation rates compared with the other group due to its gelatin shell with great biocompatibility and hydrophilicity. SEM and immunofluorescence assay results illustrated that the Coaxial-MgO scaffold significantly enhanced hPDLSCs adhesion. In vitro osteogenic and antibacterial properties showed that Coaxial-MgO membrane enhanced alkaline phosphatase (ALP) activity, formation of mineralized nodules, osteogenic-related genes [ALP, collagen type 1 (COL1), runt-related transcription factor 2 (Runx2)], and high antibacterial properties toward Escherichia coli (E. coli) and Actinobacillus actinomycetemcomitans (A. a) when compared with controls. Our findings suggested that MgO nanoparticles-incorporated coaxial electrospinning PCL-derived nanocellulose periodontal membranes might have great prospects for periodontal tissue regeneration.

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Dual effective core-shell electrospun scaffolds: Promoting osteoblast maturation and reducing bacteria activity

Cited by 21 publications

References 59 publications

The Development and Bio-applications of Multifluid Electrospinning

The Development and Bio-applications of Multifluid Electrospinning

Electrospinning: A Powerful Tool to Improve the Corrosion Resistance of Metallic Surfaces Using Nanofibrous Coatings

MgO Nanoparticles-Incorporated PCL/Gelatin-Derived Coaxial Electrospinning Nanocellulose Membranes for Periodontal Tissue Regeneration

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