Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

Peng, Zhaoxiang; Ni, Jiahua; Zheng, Kang; Shen, Yandong; Wang, Xiaoqing; He, Guo; Jin, Sung‐Ho; Tang, Tingting

doi:10.2147/ijn.s48084

Cited by 71 publications

(43 citation statements)

References 56 publications

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“…As for surface nanotopography, a previous study has demonstrated that nanoscale surfaces can stimulate cell responses without exposure to any molecular signals [ 44 ]. Similar results have been reported that the pore sizes of 15 to 80 nm of the self-assembled TiO 2 nanotube surfaces are beneficial for cell adhesion [ 45 - 47 ]. In this study, the electrochemical anodization treatment appears not to have changed the surface topography of the M specimens at the microscale but rather produced a unique hydrophilic nanonetwork structure with average pore size of approximately 70 nm and 90 nm on the anodized E1 and E2 specimens, respectively.…”

Section: Resultssupporting

confidence: 85%

Primary human nasal epithelial cell response to titanium surface with a nanonetwork structure in nasal implant applications

Yang¹,

Lan

Lee

et al. 2015

Nanoscale Res Lett

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In nasal reconstruction applications, the response of cells to titanium (Ti) implants is largely determined by the surface characteristics of the implant. This study investigated an electrochemical anodization surface treatment intended to improve the response of primary human nasal epithelial cells (HNEpC) to Ti surfaces in nasal implant applications. We used a simple and fast electrochemical anodization treatment, i.e., applying anodic current, to produce a titanium dioxide (TiO2) nanonetwork layer on the Ti surface with average lateral pore size below 100 nm, depending on the current applied. The TiO2 nanonetwork layer exhibited enhanced hydrophilicity and protein adsorption ability compared with untreated Ti surfaces. In addition, the spreading morphology, cytoskeletal arrangement, and proliferation of HNEpC on the nanonetwork layer indicated excellent cell response characteristics. This research advances our understanding regarding the means by which a TiO2 nanonetwork layer can improve the response of HNEpC to Ti surfaces in nasal implant applications.

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Section: Resultssupporting

confidence: 85%

Primary human nasal epithelial cell response to titanium surface with a nanonetwork structure in nasal implant applications

Yang¹,

Lan

Lee

et al. 2015

Nanoscale Res Lett

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“…Although it has been shown that both anatase and rutile phases possess antibacterial properties [ 39 ], underlying differences between the two possibly influence bacterial responses. Considering the common adhesion mechanism of various bacteria on implant surfaces [ 40 , 41 ], we speculate that the results observed in S. aureus may translate to other species of bacteria as well.…”

Section: Discussionmentioning

confidence: 98%

Increased Mesenchymal Stem Cell Response and DecreasedStaphylococcus aureusAdhesion on Titania Nanotubes without Pharmaceuticals

Lai²,

et al. 2015

BioMed Research International

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Titanium (Ti) implants with enhanced biocompatibility and antibacterial property are highly desirable and characterized by improved success rates. In this study, titania nanotubes (TNTs) with various tube diameters were fabricated on Ti surfaces through electrochemical anodization at 10, 30, and 60 V (denoted as NT10, NT30, and NT60, resp.). Ti was also investigated and used as a control. NT10 with a diameter of 30 nm could promote the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) without noticeable differentiation. NT30 with a diameter of 100 nm could support the adhesion and proliferation of BMSCs and induce osteogenesis. NT60 with a diameter of 200 nm demonstrated the best ability to promote cell spreading and osteogenic differentiation; however, it clearly impaired cell adhesion and proliferation. As the tube diameter increased, bacterial adhesion on the TNTs decreased and reached the lowest value on NT60. Therefore, NT30 without pharmaceuticals could be used to increase mesenchymal stem cell response and decrease Staphylococcus aureus adhesion and thus should be further studied for improving the efficacy of Ti-based orthopedic implants.

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“…The formation of bone around implants is a multistep process involving osteoblast adhesion, proliferation, differentiation, secretion of extracellular matrix proteins, and mineralization (69). Previously, some authors have reported that adhesion, proliferation, and cellular differentiation are related to the nanostructure where these cells grow (22,(70)(71)(72)(73)(74)(75), without taking into account the composition changes induced by oxide obtained by the anodization process due to the presence of different anions in the anodizing solution. The results of the present study show that the presence of F-P on the TiO 2 anodic film grown on Ti-6Al-4V alloy arguably increases the osteoblastic adhesion, growth, bone matrix secretion, and gene expression of all osteoblastic markers tested due to F-P incorporation on the Ti-6Al-4V alloy.…”

Section: Discussionmentioning

confidence: 99%

“…Titanium alloys, mainly Ti-6Al-4V, are commonly used in orthopedic surgery and traumatology due to their well-known physico-chemical properties and biocompatibility (16)(17)(18). To date, most of the methods used to manufacture nanotubular oxide layers with potential biomedical applications have been implemented in pure titanium (19)(20)(21)(22)(23)(24)(25)(26). Recently, we demonstrated that fluorine (F) doping during the anodizing process of Ti-6Al-4V decreases staphylococcal adherence by about 50% (27) and that F doping, together with the nanostructure resulting from the anodizing process, improves the osseointegrative properties by favoring matrix mineralization of osteoblastic cells (28).…”

mentioning

confidence: 99%

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

Aguilera-Correa

Mediero

Conesa-Buendía

et al. 2019

Appl Environ Microbiol

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Joint prosthesis failure is mainly related to aseptic loosening and prosthetic joint infections, both of which are associated with high morbidity and substantial costs for patients and health systems. The development of a biomaterial that is capable of stimulating bone growth while minimizing bacterial adhesion would reduce the incidence of prosthetic failure. We report antibacterial and osteostimulatory effects in a novel fluorine-phosphorus (F-P)-doped TiO2 oxide film grown on Ti-6Al-4V alloy with a nanostructure of bottle-shaped nanotubes (bNT) using five bacterial species (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Stenotrophomonas maltophilia) and MCT3T3-E1 osteoblastic cells. The interaction between the bacteria and bNT Ti-6Al-4V was complex, as the adhesion of four bacterial species decreased (two staphylococcus species, E. coli, and S. maltophilia), and the viability of staphylococci and S. maltophilia also decreased because of the aluminum (Al) released by bNT Ti-6Al-4V. This released Al can be recruited by the bacteria through siderophores and was retained only by the Gram-negative bacteria tested. P. aeruginosa showed higher adhesion on bNT Ti-6Al-4V than on chemically polished (CP) samples of Ti-6Al-4V alloy and an ability to mobilize Al from bNT Ti-6Al-4V. The cell adhesion and proliferation of MCT3T3-E1 osteoblastic cells significantly increased at 48 and 168 h, as did the matrix mineralization of these cells and the gene expression levels of three of the most important markers related to bone differentiation. According to our results, the bNT Ti-6Al-4V alloy could have clinical application, preventing infection and stimulating bone growth and thus preventing the two main causes of joint prosthesis failure. IMPORTANCE This work evaluates F-P-doped bNT Ti-6Al-4V from microbiological and cellular approaches. The bacterial results highlight that the antibacterial ability of bNT Ti-6Al-4V is the result of a combination of antiadhesive and bactericidal effects exerted by Al released from the alloy. The cell results highlight that F-P bNT Ti-6Al-4V alloy increases osseointegration due to modification of the chemical composition of the alloy resulting from P incorporation and not due to the nanostructure, as reported previously. A key finding was the detection of Al release from inside the bNT Ti-6Al-4V nanostructures, a result of the nanostructure growth during the anodizing process that is in part responsible for its bactericidal effect.

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Dual effects and mechanism of TiO2 nanotube arrays in reducing bacterial colonization and enhancing C3H10T1/2 cell adhesion

Cited by 71 publications

References 56 publications

Primary human nasal epithelial cell response to titanium surface with a nanonetwork structure in nasal implant applications

Primary human nasal epithelial cell response to titanium surface with a nanonetwork structure in nasal implant applications

Increased Mesenchymal Stem Cell Response and DecreasedStaphylococcus aureusAdhesion on Titania Nanotubes without Pharmaceuticals

Microbiological and Cellular Evaluation of a Fluorine-Phosphorus-Doped Titanium Alloy, a Novel Antibacterial and Osteostimulatory Biomaterial with Potential Applications in Orthopedic Surgery

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