Dual effects of ATP on isolated arteries of the bovine eye

Ziganshina, Anna; Ziganshin, Bulat A.; Зиганшин, А. У.

doi:10.1016/j.phrs.2012.04.002

Cited by 4 publications

(4 citation statements)

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“…10 Here, the dilatation induced when the purine was applied to the extravascular part of the excised vascular segment may have been supplemented with a constrictive effect when the compound could enter the vascular lumen from the open ends of the studied vascular segments. Differences in the balance between these constricting and dilating effects on retinal vessels may explain species variations so that extravascular application of ATP can induce constriction in the rat 4,5 and cow, 6,7 and dilatation in pigs. 31 Dual effects of vasoactive compounds have previously been shown in the eye 32 as well as in extraocular tissues, 33,34 FIGURE 6.…”

Section: Discussionmentioning

confidence: 99%

“…KPSS was PSS 1. 6 where NaCl had been replaced with equimolar amounts of KCl resulting in a K + concentration of 124 mmol/l. PSS-HEPES was PSS 1.6 without KH 2 PO 4 , EDTA, and HEPES reduced to a concentration of 5.0 (pH = 7.53).…”

Section: Solutionsmentioning

confidence: 99%

“…3 Animal studies have shown that extravascular application of ATP induces contraction of rat vascular pericytes 4 and arterioles 5 that are mediated by P2X receptors. In bovine tissue, ATP can induce contraction of arterioles 6 and retinal vascular pericytes. 7 In porcine retinal arterioles, extravascular application of ATP agonists induces vasodilatation 8,9 and the ATP degradation product adenosine has dual effects on the tone of retinal arterioles with contraction and relaxation mediated by different receptors.…”

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confidence: 99%

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Differential Effects of Intra- and Extravascular ATP on the Diameter of Porcine Vessels at Different Branching Levels Ex Vivo

Ernst

Jensen

Aalkjær

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. Adenosine triphosphate (ATP) is involved in the diameter regulation of retinal vessels. The compound has been shown to induce both constriction and dilatation, but the detailed mechanisms underlying these effects and the site of action of the compound are not known in detail. Therefore, the purpose of the present study was to investigate whether the vasoactive effects of ATP on retinal vessels depend on intra-and extravascular application, and to study whether the effects differ at different vascular branching levels. METHODS. Diameter changes in arterioles, pre-capillary arterioles, and capillaries were studied in perfused porcine hemiretinas (n = 48) ex vivo after intra-and extravascular application of the nondegradable ATP analogue ATP-γ-S or ATP in the presence or not of antagonists to the CD73/ecto-5-nucleotidase (AOPCP), the P2-purinergic receptor (PPADS), the A3-adenosine receptor (MRS1523), and the synthesis of cyclooxygenase products (ibuprofen). RESULTS. Intravascular ATP-induced constriction and extravascular ATP-induced dilatation of retinal arterioles, pre-capillary arterioles and capillaries, and dilatation was inhibited by ibuprofen. Both constriction and dilatation of arterioles were inhibited by antagonizing ATP degradation. Furthermore, constriction at all three branching levels was antagonized by blocking the A3 purinoceptor, whereas constriction in arterioles and pre-capillary arterioles was antagonized by blocking the P2 purinoceptor. CONCLUSIONS. ATP affects the diameter of retinal arterioles, pre-capillary arterioles, and capillaries through different pathways, and the effects depend on whether the compound is administered intravascularly or extravascularly. This may form the basis for selective interventions on retinal vascular disease with differential involvement of vessels at different branching levels.

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Section: Discussionmentioning

confidence: 99%

Section: Solutionsmentioning

confidence: 99%

mentioning

confidence: 99%

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Differential Effects of Intra- and Extravascular ATP on the Diameter of Porcine Vessels at Different Branching Levels Ex Vivo

Ernst

Jensen

Aalkjær

et al. 2020

Invest. Ophthalmol. Vis. Sci.

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show abstract

“…Purinoceptors have been shown to be involved in the regulation of local blood flow in the eye (Ziganshina et al, 2012). ATP induced contraction, probably via P2X 1 receptors, in bovine ophthalmic and posterior ciliary arteries and relaxation, probably via P1 receptors.…”

Section: R Other Blood Vesselsmentioning

confidence: 99%