1998
DOI: 10.1038/sj.bjp.0701620
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Dual effects of histamine and substance P on intracellular calcium levels in human U373 MG astrocytoma cells: role of protein kinase C

Abstract: 1 In human U373 MG astrocytoma cells agonist-induced increases in intracellular Ca 2+ ([Ca 2+ ] i ) are rapidly returned towards prestimulated levels. Examination of the e ect of histamine and substance P on [Ca 2+ ] i in thapsigargin-treated cells has allowed a mechanism contributing to this e ect to be characterized. 4 Treatment of U373 MG cells with 5 mM thapsigargin, followed by the readdition of 1.8 mM Ca 2+ to the perfusion bu er, resulted in a steady-state level of [Ca 2+ ] i 97+5 nM above pretreated… Show more

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Cited by 15 publications
(17 citation statements)
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“…Histamine (10 mM) acting alone had no eect on cyclic AMP accumulation, consistent with our earlier observations on dissociated U373 MG cells (Young et al, 1998a), but produced a marked inhibition of the response to isoprenaline (Figure 2). The inhibitory eect of histamine was completely dependent on the presence of Ca 2+ in the extracellular medium.…”
Section: + -Dependent Inhibition Of Cyclic Amp Accumulation In U373 supporting
confidence: 80%
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“…Histamine (10 mM) acting alone had no eect on cyclic AMP accumulation, consistent with our earlier observations on dissociated U373 MG cells (Young et al, 1998a), but produced a marked inhibition of the response to isoprenaline (Figure 2). The inhibitory eect of histamine was completely dependent on the presence of Ca 2+ in the extracellular medium.…”
Section: + -Dependent Inhibition Of Cyclic Amp Accumulation In U373 supporting
confidence: 80%
“…U373 MG astrocytoma cells were cultured and dissociated as described previously (Young et al, 1998a). The cells were seeded onto 6-well culture plates at a density of approximately 7610 4 cells well 71 and grown to near con¯uence.…”
Section: Measurement Of [ 3 H]-cyclic Amp Accumulationmentioning
confidence: 99%
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