Dual-emissive fluorescence measurements of hydroxyl radicals using a coumarin-activated silica nanohybrid probe

Abstract

This work reports a novel dual-emissive fluorescent probe based on dye hybrid silica nanoparticles for ratiometric measurement of the hydroxyl radical (˙OH). In the probe sensing system, the blue emission of coumarin dye (coumarin-3-carboxylic acid, CCA) immobilized on the nanoparticle surface is selectively enhanced by ˙OH due to the formation of a coumarin hydroxylation product with strong fluorescence, whereas the emission of red fluorescent dye encapsulated in the silica nanoparticle is insensitive to ˙OH as a self-referencing signal, and so the probe provides a good quantitative analysis based on ratiometric fluorescence measurement with a detection limit of 1.65 μM. Moreover, the probe also shows high selectivity for ˙OH determination against metal ions, other reactive oxygen species and biological species. More importantly, it exhibits low cytotoxicity and high biocompatibility in living cells, and has been successfully used for cellular imaging of ˙OH, showing its promising application for monitoring of intracellular ˙OH signaling events.

Sensitive determination of endogenous hydroxyl radical in live cell by a BODIPY based fluorescent probe

“…Yield (35.9 mg, 29.5%). HR-MS (m/z, ESI) calculated for C 20 [4,[49][50][51]. Tert-butyl peroxyl radical (TBO•) was generated in situ by addition of Fe 2+ (300 μM) in the presence of TBHP (6 mM) [31].…”

“…Reactive oxygen species (ROS) have been of widespread attention on account of its important roles in the physiology and pathology [1][2][3][4]. These small molecules usually relate to mediating redox modifications of biological molecules and probably implicate in aging and various kinds of pathological types, such as cancer, diabetes, neurodegenerative diseases, cardiovascular and inflammatory diseases [5][6][7][8][9].…”

“…Since hydroxyl radical is shortlived and reactive, its real-time sensitive and selective detection methods are still in high demand. Currently, there are some methods for the detection of hydroxyl radical, including electron spin resonance [13][14][15][16], nuclear magnetic resonance [17], HPLC method [18], UV-vis spectroscopy and spectrofluorometry [4,19], electrochemical method [20] and chemiluminescence [21]. Compared with those conventional methods, fluorescence method possesses the advantages of simplicity, sensitivity and the visible changes of fluorescence color potential for visual detection of the target analyte [22][23][24][25][26][27][28][29][30].…”

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

“…Additionally, nanoparticle‐based OH • probes have been developed. Wang and co‐workers reported a coumarin‐based silica nanohybrid as a fluorescent probe to measure OH • . The nanoparticle was formed by organic silica molecules with coumarin grafted onto the surface ( Figure ).…”

“…Construction of a nanoparticle‐based OH • probe and its sensing process. Reproduced with permission . Copyright 2016, The Royal Society of Chemistry.…”