Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine

Henao-Escobar, Wilder; Román, Lorena del Torno‐de; Domínguez‐Renedo, Olga; Alonso‐Lomillo, M. Asunción; Arcos‐Martínez, M. Julia

doi:10.1016/j.foodchem.2015.06.035

Cited by 72 publications

(24 citation statements)

References 39 publications

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“…Although partial extraction was achieved, the results correlated well with total biogenic amine content measured by HPLC method [44]. An equivalent determination has been reported very recently from the bi-amperometric measurement of a double enzyme biosensor using histamine dehydrogenase and putrescine oxidase [45] that performs the specific determination of histamine and putrescine, working simply at two fixed potentials of +130 and +300 mV, respectively, in this case, without the need of any chemometric contribution.…”

Section: Determining One Substratesupporting

confidence: 55%

Bioelectronic Tongues Employing Electrochemical Biosensors

Valle

2016

Bioanalytical Reviews

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This chapter presents recent advances concerning work with electronic tongues that employ electrochemical biosensors, that is, bioelectronic tongues. (Bio)electronic tongues represent a new methodological use of (bio)sensors; they start by the use of biosensor arrays and assume the coupling of the obtained complex response with advanced chemometric data treatment; the goal is improving performance of existing sensors. Most of the bioelectronic tongues reported employ enzyme biosensors, essentially based on potentiometric or voltammetric/ amperometric transduction. This report is organized considering the different forms to incorporate biosensors, i.e. considering the number of biosensors in the array, the number of different enzymes used, if the determination is aimed to substrates or inhibitors, etc. Significant applications in real problem-solving, mainly in the food and clinical or environmental fields, are commented.

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Section: Determining One Substratesupporting

confidence: 55%

Bioelectronic Tongues Employing Electrochemical Biosensors

Valle

2016

Bioanalytical Reviews

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“…Moreover, screen-printed electrodes (SPEs) are appropriate platforms for the immobilization of biomolecules, e.g. nucleic acid (Das et al 2014), enzymes (Henao-Escobar et al 2016) or antibodies (Ojeda et al 2015) onto the underlying working electrode surface in order to obtain a sensitive, selective, disposable electrochemical biosensor. SPE platforms are mostly based on carbon materials in which the nature, structural and physic-chemical properties of the carbonaceous materials have paid significant attention to the performance of the electrochemical (bio)sensors.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Hernández-Ibáñez

García‐Cruz

Montiel

et al. 2016

Biosensors and Bioelectronics

142

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Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures, Biosensors and Bioelectronic, http://dx.doi.org/10.1016/j.bios.2015.11.005 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. This novel electrochemical lactate biosensors analytical efficacy is explored towards the sensing of lactate in model (buffer) solutions and is found to exhibit a linear response towards lactate over the concentration range of 30.4 and 243.9 µM in phosphate buffer solution, with a corresponding limit of detection (based on 3-sigma) of 22.6 µM and exhibits a sensitivity of 3417 ± 131 µA M -1 according to the reproducibility study. These novel electrochemical lactate biosensors exhibit a high reproducibility, with a relative standard deviation of less than 3.8 % and an enzymatic response over 82 % after 5 months stored at 4 ºC. Furthermore, high performance liquid chromatography technique has been utilized to independently validate the electrochemical lactate biosensor for the determination of lactate in a commercial embryonic cell culture medium providing excellent agreement between the two analytical protocols.

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“…Histamine can cause the aggregation of the unmodified AuNPs via replacing the adsorbed citrate ions, which triggers a visible color change and allows the detection of histamine by bare eyes. The minimum visually detectable histamine concentration by bare eyes is 1.8 μM, which is comparable to the limit of detection (LOD) of the electrochemical-based approaches [6,13,14,17] at a signal-to-noise ratio of 3:1. The LOD of our approach is decreased to 38 nM when using absorbance at 522 nm.…”

Section: Electronic Supplementary Materialsmentioning

confidence: 83%

“…Histamine in food can be detected by chromatographic techniques [7][8][9], capillary electrophoresis [10,11], surface plasmon resonance [12], immunoassays [12,13], molecule imprints [14,15], and electrochemical techniques [6,13,14,[16][17][18]. The typical performances of these approaches are summarized in Table 1.…”

Section: Introductionmentioning

confidence: 99%

Visual and photometric determination of histamine using unmodified gold nanoparticles

et al. 2017

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The authors describe a colorimetric approach for on-site monitoring of histamine based on the use of gold nanoparticles (AuNPs) that have a negatively charged surface due to the presence of adsorbed citrate ions. Histamine has two basic functional groups, an aliphatic amino group and an imidazole ring. Under the experimental conditions, the protonated aliphatic amino group drives the imidazole ring into close proximity to the AuNPs due to electrostatic attraction. This accelerates the replacement of citrate ions by the imidazole ring because of the strong affinity between imidazole and AuNPs. As a consequence, the two groups synergistically induce the aggregation of the AuNPs and trigger a visible color change from red to blue. The minimum visually detectable histamine concentration is 1.81 μM, which is comparable to the limit of detection (LOD) of the electrochemical approaches at a signal-to-noise ratio of 3:1. When using absorbance at 522 nm, the LOD is lowered to 38 nM. The method was applied to the determination of histamine in fish samples.

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Dual enzymatic biosensor for simultaneous amperometric determination of histamine and putrescine

Cited by 72 publications

References 39 publications

Bioelectronic Tongues Employing Electrochemical Biosensors

Bioelectronic Tongues Employing Electrochemical Biosensors

Electrochemical lactate biosensor based upon chitosan/carbon nanotubes modified screen-printed graphite electrodes for the determination of lactate in embryonic cell cultures

Visual and photometric determination of histamine using unmodified gold nanoparticles

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