Dual‐enzyme, co‐immobilized capillary microreactor combined with substrate recycling for high‐sensitive glutamate determination based on CE

Yang, Li; Shi, Jing; Chen, Cuijie; Wang, Shaoming; Zhu, Lihua; Xie, Wei; Guo, Liping

doi:10.1002/elps.200900195

Cited by 19 publications

(14 citation statements)

References 30 publications

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“…Glucose-6-phosphate dehydrogenase Silica-based hydrogels Immobilized in a hydrogel obtained by sol-gel method from a mixture containing precursors, APTES and the enzyme [142,143]. Glutamate dehydrogenase Fused-silica capillary Immobilized together with a transaminase by the APTES/GA method [131]. Invertase Silica monoliths Immobilized on silica rods previously activated by GA [144].…”

Section: Enzyme(s) Solid Support Methods Of Enzyme Immobilizationmentioning

confidence: 99%

“…Despite the increased complexity of construction, this kind of structure is advantageous because it does not have the problems typically associated with the multiple microreactor assembly, such as fluid or electric leakage. These systems have typically been used for kinetic or inhibition studies [127][128][129] and quantitative determinations of metabolites [119,130,131].…”

Section: Magnetic Beadsmentioning

confidence: 99%

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Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

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Microfluidic reaction devices are a very promising technology for chemical and biochemical processes. In microreactors, the micro dimensions, coupled with a high surface area/volume ratio, permit rapid heat exchange and mass transfer, resulting in higher reaction yields and reaction rates than in conventional reactors. Moreover, the lower energy consumption and easier separation of products permit these systems to have a lower environmental impact compared to macroscale, conventional reactors. Due to these benefits, the use of microreactors is increasing in the biocatalysis field, both by using enzymes in solution and their immobilized counterparts. Following an introduction to the most common applications of microreactors in chemical processes, a broad overview will be given of the latest applications in biocatalytic processes performed in microreactors with free or immobilized enzymes. In particular, attention is given to the nature of the materials used as a support for the enzymes and the strategies employed for their immobilization. Mathematical and engineering aspects concerning fluid dynamics in microreactors were also taken into account as fundamental factors for the optimization of these systems.

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Section: Enzyme(s) Solid Support Methods Of Enzyme Immobilizationmentioning

confidence: 99%

Section: Magnetic Beadsmentioning

confidence: 99%

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Laurenti¹,

Vianna²

2016

Biocatalysis

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“…Genetically, engineered transaminases have demonstrated excellent performance in the synthesis of important drug scaffolds [5]. In addition, keeping the recyclability issue in mind, researchers have proposed successful immobilization strategies for transaminases using for example natural and synthetic polymers [8][9][10][11][12] or silica [13][14][15] as carriers.…”

Section: Introductionmentioning

confidence: 99%

Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith

2017

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ω-Transaminases have been immobilized on macrocellular silica monoliths and used as heterogeneous biocatalysts in a continuous flow mode enantioselective transamination reaction. The support was prepared by a sol-gel method based on emulsion templating. The enzyme was immobilized on the structured silica monoliths both by adsorption, and by covalent grafting using amino-functionalized silica monoliths and glutaraldehyde as a coupling agent. A simple reactor set-up based on the use of a heat-shrinkable Teflon tube is presented and successfully used for the continuous flow kinetic resolution of a chiral amine, 4-bromo-α-methylbenzylamine. The porous structure of the supports ensures effective mass transfer and the reactor works in the plug flow regime without preferential flow paths. When immobilized in the monolith and used in the flow reactor, transaminases retain their activity and their enantioselectivity. The solid biocatalyst is also shown to be stable both on stream and during storage. These essential features pave the way to the successful development of an environmentally friendly process for chiral amines production.

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“…Genetically engineered transaminases have demonstrated excellent performance in the synthesis of important drug scaffolds [8]. Also, keeping the recyclability issue in mind, researchers have proposed successful immobilization strategies for transaminases using for example natural and synthetic polymers [11][12][13][14][15] or silica [16][17][18] as carriers.…”

Section: Introductionmentioning

confidence: 99%

Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith

Biggelaar¹,

Soumillion²,

Debecker³

2017

Preprint

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ω-Transaminases have been immobilized on macrocellular silica monoliths and used as heterogeneous biocatalysts in a continuous flow mode enantioselective transamination reaction. The support was prepared by a sol-gel method based on emulsion-templating. The enzyme was immobilized on the structured silica monoliths both by adsorption, and by covalent grafting using amino-functionalized silica monoliths and glutaraldehyde as a coupling agent. A simple reactor set-up based on the use of a heat-shrinkable Teflon tube is presented and successfully used for the continuous flow kinetic resolution of a chiral amine, 4-bromo-α-methylbenzylamine. The porous structure of the supports ensures effective mass transfer and the reactor works in the plug flow regime without preferential flow paths. When immobilized in the monolith and used in the flow reactor, transaminases retain their activity and their enantioselectivity. The solid biocatalyst is also shown to be stable both on stream and during storage. These essential features pave the way to the successful development of an environmentally friendly process for chiral amines production.

show abstract

Dual‐enzyme, co‐immobilized capillary microreactor combined with substrate recycling for high‐sensitive glutamate determination based on CE

Cited by 19 publications

References 30 publications

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Enzymatic microreactors in biocatalysis: history, features, and future perspectives

Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith

Enantioselective Transamination in Continuous Flow Mode with Transaminase Immobilized in a Macrocellular Silica Monolith

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