Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Chen, Jiangbo; Zhang, Yachao; Bai, Songnan; Zhu, Jingyi; Chirarattananon, Pakpong; Ni, Kai; Zhou, Qian; Wang, Lidai

doi:10.1016/j.pacs.2021.100292

Cited by 15 publications

(9 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Third, we optimize the fabrication process of the optical/acoustic combiner to reduce acoustic signal loss in transmission. Compared with our previous system 20,48 , the new probe increases the signal magnitude by ~ 300% (Supplementary Fig. S2b and S2c).…”

Section: Methodsmentioning

confidence: 70%

“…High detection sensitivity is not only important for high-quality imaging, but also enables the detection of more low-absorbing chromophores at a lower concentration, reduces photobleaching, toxicity, or perturbation, and allows for more choices of low-cost low-power lasers at a broader spectrum. For instance, in ophthalmologic examination 16 , low laser energy is preferred for more safety and comfort; in high-speed OR-PAM, increased pulse repetition rates (PRR) limits the laser pulse energy [17][18][19][20] ; wearable or portable OR-PAM prefers lightweight low-power lasers 21 ; long-term monitoring of pharmacokinetics or hemodynamics requires low-dose imaging to reduce photobleaching or phototoxicity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Super-low-dose functional and molecular photoacoustic microscopy

Zhang

CHEN

ZHANG

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

Photoacoustic microscopy can image various biological molecules and nano-agents in vivo via low-scattering ultrasonic sensing. Insufficient sensitivity has been a long-standing obstacle for imaging of many low-absorbing chromophores with less photobleaching or toxicity, reduced perturbation to delicate organs, and more choices of lasers. Here, we present a multi-spectral super-low-dose photoacoustic microscopy (SLD-PAM) that is up to ~ 33-times more sensitive than the state of the art. We collaboratively innovate the photoacoustic probe design and implement a spectral-spatial filter. The new probe collects 300% more acoustic signals, and the filter further improves the signal-to-noise ratio by 200%~1100% across all optical wavelengths. SLD-PAM can visualize microvessels and quantify oxygen saturation (sO2) in vivo with ~ 1% of the maximum permissible exposure, dramatically reducing any possible phototoxicity or perturbation, especially in imaging of the eye and the brain. Capitalizing on the high sensitivity, we, for the first time, implement direct imaging of deoxyhemoglobin (Deoxy-Hb) concentration without spectral unmixing, avoiding wavelength-dependent and computational noises. We show that super-low-dose molecular imaging can reduce photobleaching by at least 85%. We also demonstrate SLD-PAM achieves similar molecular imaging quality using 80% fewer contrast agents, which greatly reduces potential biotoxicity. SLD-PAM enables the use of more low-absorbing nano-agents, small molecules, and genetically-encoded biomarkers, as well as more low-power light sources at wider spectra. We believe SLD-PAM offers a powerful tool for anatomical, functional, and molecular imaging.

show abstract

Section: Methodsmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Super-low-dose functional and molecular photoacoustic microscopy

Zhang

CHEN

ZHANG

et al. 2022

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…However, OR-PAM faces several technical challenges [13] , [14] , [15] , [16] . First, the field of view is small [17] , [18] .…”

Section: Introductionmentioning

confidence: 99%

Freehand scanning photoacoustic microscopy with simultaneous localization and mapping

Chen

Zhang²,

Zhu³

et al. 2022

Photoacoustics

Self Cite

View full text Add to dashboard Cite

“…[9][10][11][12] Because PAI offers high imaging resolution in deep tissue and FLI provides high imaging sensitivity, the combination of the two modalities can accurately detect exogenous and endogenous contrasts including oxygen saturation, lipids, hemoglobin concentration, and a variety of molecules and nanoparticles. [13][14][15][16][17] Meanwhile, due to the high photothermal conversion efficiency (PCE) and efficient reactive oxygen species (ROS) generation of some optical contrast agents, the combination of photothermal therapy (PTT)/photodynamic therapy (PDT) can be readily co-implemented under the real-time guidance by optical imaging, which provides an excellent adjuvant strategy to efficient ablation and hypoxia amelioration for tumor treatment. [18][19][20][21][22] Unfortunately, limited kinds of nano-agents are practicable to achieve the precision diagnosis and phototherapeutic for SHCC.…”

Section: Introductionmentioning

confidence: 99%

Targeted Multifunctional Nanoplatform for Imaging-Guided Precision Diagnosis and Photothermal/Photodynamic Therapy of Orthotopic Hepatocellular Carcinoma

Qi¹,

Liu²,

Chen³

et al. 2022

IJN

Self Cite

View full text Add to dashboard Cite

Background Effective theranostic of hepatocellular carcinoma (HCC) in an early-stage is imminently demanded to improve its poor prognosis. Combination of the near-infrared (NIR) photoacoustic imaging (PAI) and fluorescence imaging (FLI) can provide high temporospatial resolution, outstanding optical contrast, and deep penetration and thus is promising for accurate and sensitive HCC diagnosis. Methods A versatile CXCR4-targeted Indocyanine green (ICG)/Platinum (Pt)-doped polydopamine melanin-mimic nanoparticle (designated ICG/Pt@PDA-CXCR4, referred to as IPP-c) is synthesized as an HCC-specific contrast agent for high-resolution precise diagnostic PAI/FLI and optical imaging-guided targeted photothermal therapy (PTT)/photodynamic therapy (PDT) of orthotopic small hepatocellular carcinoma (SHCC). Results The multifunctional targeted nanoparticle yields superior HCC specificity, high imaging contrast in both PAI and FLI, good stability, reliable biocompatibility, effective singlet oxygen generation and superior photothermal conversion efficiency (PCE, 58.7%) upon 808-nm laser irradiation. The targeting ability of IPP-c was validated in in vitro experiments on selectively killing the CXCR4-overexpressing HCC cells. Moreover, we test the efficient dual-modal optical precision diagnosis properties of IPP-c via in vivo experiments on targeted particle accumulation in an early-stage SHCC mouse model (tumor diameter about 1.2 mm). Then, under the guidance of real-time optical imaging, effective and mini-invasive PTT/PDT of orthotopic SHCCs were demonstrated without damaging adjacent liver tissues or other major organs. Conclusion This study presented a multifunctional CXCR4-targeted nanoparticle to conduct effective and mini-invasive phototherapeutics of orthotopic SHCCs via the real-time quantitative guidance by optical imaging, which provided a new perception for building a versatile targeted nanoplatform for phototheranostics of early-stage HCC.

show abstract

Dual-foci fast-scanning photoacoustic microscopy with 3.2-MHz A-line rate

Cited by 15 publications

References 46 publications

Super-low-dose functional and molecular photoacoustic microscopy

Super-low-dose functional and molecular photoacoustic microscopy

Freehand scanning photoacoustic microscopy with simultaneous localization and mapping

Targeted Multifunctional Nanoplatform for Imaging-Guided Precision Diagnosis and Photothermal/Photodynamic Therapy of Orthotopic Hepatocellular Carcinoma

Contact Info

Product

Resources

About