Dual frequency cavitation event sensor with iodide dosimeter

Ebrahiminia, Ali; Mokhtari‐Dizaji, Manijhe; Toliyat, Tayebeh

doi:10.1016/j.ultsonch.2015.07.005

Cited by 29 publications

(18 citation statements)

References 27 publications

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“…The cavitational activity of the different coupling media used in the LFU experiments was estimated using potassium iodide (KI) dosimeter as a standard method. 34,35 In this method, when KI solution is sonicated, the generated reactive radicals oxidize the I − to produce I 2 . The excess of I − reacts with I 2 to form triiodide (I − 3 ).…”

Section: Cavitational Activity Of Dspe-peg Micelles Potassium Iodide mentioning

confidence: 99%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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Purpose: Sonodynamic therapy (SDT) is a new therapeutic modality for the noninvasive cancer treatment based on the association of ultrasound and sonosensitizer drugs. Topical SDT requires the development of delivery systems to properly transport the sonosensitizer, such as zinc phthalocyanine (ZnPc), to the skin. In addition, the delivery system itself can participate in sonodynamic events and influence the therapeutic response. This study aimed to develop ZnPc-loaded micelle to evaluate its potential as a topical delivery system and as a cavitational agent for low-frequency ultrasound (LFU) application with the dual purpose of promoting ZnPc skin penetration and generating reactive oxygen species (ROS) for SDT. Methods: ZnPc-loaded micelles were developed by the thin-film hydration method and optimized using the Quality by Design approach. Micelles' influence on LFU-induced cavitation activity was measured by potassium iodide dosimeter and aluminum foil pits experiments. In vitro skin penetration of ZnPc was assessed after pretreatment of the skin with LFU and simultaneous LFU treatment using ZnPc-loaded micelles as coupling media followed by 6 h of passive permeation of ZnPc-loaded micelles. The singlet oxygen generation by LFU irradiation of the micelles was evaluated using two different hydrophilic probes. The lipid peroxidation of the skin was estimated using the malondialdehyde assay after skin treatment with simultaneous LFU using ZnPc-loaded micelles. The viability of the B16F10 melanoma cell line was evaluated using resazurin after treatment with different concentrations of ZnPc-loaded micelles irradiated or not with LFU. Results: The micelles increased the solubility of ZnPc and augmented the LFU-induced cavitation activity in two times compared to water. After 6 h ZnPc-loaded micelles skin permeation, simultaneous LFU treatment increased the amount of ZnPc in the dermis by more than 40 times, when compared to non-LFU-mediated treatment, and by almost 5 times, when compared to LFU pretreatment protocol. The LFU irradiation of micelles induced the generation of singlet oxygen, and the lipoperoxidation of the skin treated with the simultaneous LFU was enhanced in three times in comparison to the non-LFU-treated skin. A significant reduction in cell viability following treatment with ZnPc-loaded micelles and LFU was observed compared to blank micelles and non-LFU-treated control groups. Conclusion: LFU-irradiated mice can be a potential approach to skin cancer treatment by combining the functions of increasing drug penetration and ROS generation required for SDT.

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Section: Cavitational Activity Of Dspe-peg Micelles Potassium Iodide mentioning

confidence: 99%

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Martins¹,

Fonseca²,

Pavan³

et al. 2020

IJN

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“…In physiological conditions of 37 C and using low intensity, non-thermal effects can be the dominant effect and the main interaction ie, acoustic cavitation is important. According to the studies, 22,23,32,33 the stable cavitation without collapse and the ability to apply stress without chemical activities produces beneficial effects in low intensity and low frequency. The phenomenon of acoustic cavitation is quantified by the mechanical index parameter.…”

Section: Discussionmentioning

confidence: 99%

Effect of Acoustic Cavitation on Mouse Spermatogonial Stem Cells

Moghaddam

Mokhtari‐Dizaji

Movahedin

2020

J of Ultrasound Medicine

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Objectives The mechanical index has long been one of the main criteria used to assess the safety limits for therapeutic medical applications. However, the safety of the mechanical index parameter is considered to be unknown in male fertility, which has a very significant role in vitro conditions. In this study, the effect of cavitation interactions due to mechanical index regions was evaluated on spermatogonial stem cells. Methods The acoustic pressure and mechanical index equations at the low intensities and the intended frequency were modeled and solved. The mechanical index average of 40 kHz frequency was selected as subthreshold, 0.70, and above the cavitation threshold. Neonatal spermatogonial stem cells were cultured. Spermatogonial stem cells are stimulated by low‐level ultrasound for 5 days and colonization and viability evaluated on the seventh day. Results Based on modeling, the mechanical index average was chosen as 0.40, 0.51, 0.75, and 0.89. The mechanical index of 0.40 and 0.89 resulted in a number of colonies of 93 ± 4 and 32 ± 4, respectively. An increase in colony diameter could be observed for a 0.40 mechanical index during all days of the culture that in the culture on the seventh day had the largest average colony diameter of 174.05 ± 1.22 μm in comparison with other groups (p < 0.05). The cell viability was not significantly different among the groups. Conclusion The results suggest that a low‐intensity ultrasound of 40 kHz with a 0.40 mechanical index can be effective in increasing the proliferation and colonization of spermatogonia in stem cells during culture.

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“…To assay inertial cavitation activity, KI dosimetry was used 3,22 . After sonication of the KI solution, sonochemical reactions led to triiodide ion (I −3 ) formation.…”

Section: Methodsmentioning

confidence: 99%

The effect of dual‐frequency ultrasound waves on B16F10 melanoma cells: Sonodynamic therapy using nanoliposomes containing methylene blue

Adelnia

Mokhtari‐Dizaji

Hoseinkhani

et al. 2020

Skin Research and Technology

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Background We investigated the effect of dual‐frequency sonication on the viability of B16F10 melanoma cells in the presence of methylene blue (MB) encapsulated in nanoliposomes. Methods Treatment protocols were studied: sonication groups (40 kHz, 1 MHz and dual‐frequency), the same sonication groups with nanoliposomes containing MB, MB free and nanoliposomes containing MB groups, and so sham and control groups. The nanoliposomes were prepared by the lipid film hydration method. The cell viability of the different treatment groups was evaluated by the MTT assay. Results The dual‐frequency protocols caused higher viability losses compared to the kHz and MHz sonications (P < .05). In presence of the nanoliposomes containing MB, dual frequency led to 6% and 3% viability for 600 and 1200 seconds, respectively, while the corresponding values were 10% and 4% for the 40 kHz protocols and 22% and 9% for the 1 MHz, as compared to the control group (100%). The result of KI dosimetry showed that the cavitation activity of the dual‐frequency protocol was about 1.23, as compared to sonication at 40 kHz and 1 MHz. Conclusion Enhancement of inertial cavitation induction by dual‐frequency sonication may be the primary effective mechanism, which causes increased sonochemical processes and drug release from nanocarriers.

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Dual frequency cavitation event sensor with iodide dosimeter

Cited by 29 publications

References 27 publications

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

<p>Bifunctional Therapeutic Application of Low-Frequency Ultrasound Associated with Zinc Phthalocyanine-Loaded Micelles</p>

Effect of Acoustic Cavitation on Mouse Spermatogonial Stem Cells

The effect of dual‐frequency ultrasound waves on B16F10 melanoma cells: Sonodynamic therapy using nanoliposomes containing methylene blue

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