The exposure of individuals to mechanical vibrations produced in vibrating platform can generate whole-body vibration (WBV) exercise. This modality of exercise represents a less tiring alternative to sport, which could benefit mechanical human behavior and prevent sports injuries and musculoskeletal disorders. The specificity of the human body, articulated and connected segments, requires to take into account the biomechanical parameters in protocols involving WBV exercise. Moreover, work time, rest time between the bouts in each session number of sessions, extension of the intervention, and week periodicity must be also well established. Responses to WBV exercise are observed at musculoskeletal, neurological, endocrinological and vascular levels. With respect to the musculoskeletal level, it is verified increase of muscle strength, endurance and power, improvement of the balance, increase of bone mineral density and the decrease of risk of falls. There are several tools to evaluate the responses of the body to WBV exercise. The aim of this chapter is to highlight the relevance of the WBV exercise as an intervention of the physical therapy for the better human optimization.