Dual function of active constituents from bark of Ficus racemosa L in wound healing

Bopage, Nisansala Swarnamali; Gunaherath, G. M. Kamal B.; Jayawardena, K.H.; Wijeyaratne, Sushila Chandrani; Abeysekera, A.M.; Somaratne, S.

doi:10.1186/s12906-018-2089-9

Cited by 27 publications

(17 citation statements)

References 31 publications

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“…They include P. granatum, P. guajava, C. arborea, G. decora, S. obtusa, and F. hispida. The findings about the potential effectiveness of these plants are similar to those found in the in vivo and in vitro studies previously reported [31][32][33][34][35][36][37][38][39][40]. Leaves from P. granatum and P. guajava and pieces of bark from C. arborea, G. decora, S. obtusa, and F. hispida were collected in Baan Tham, Dok Kham Tai, Phayao.…”

Section: Collection and Preparation Of The Extractssupporting

confidence: 82%

Anti-Inflammatory and Antioxidant Activities of Medicinal Plants Used by Traditional Healers for Antiulcer Treatment

et al. 2019

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For centuries, many kinds of native plants and their products have been used for the treatment of gastric ulcers by traditional healers in Phayao province. The current study aimed to investigate the polyphenol content in some of these medicinal plants and to point out the relationship between their antioxidant capacity and anti-inflammatory activities. Six species were selected based on ethnopharmacologic considerations: Punica granatum L., Psidium guajava L., Careya arborea Roxb., Gochnatia decora (Kurz) Cabr., Shorea obtusa Wall. ex Blume, and Ficus hispida L.f. The leaves or bark of these plants were extracted with 70% ethanol and water. Anti-inflammatory and antioxidant activities of the extracts were analyzed based on nitric oxide (NO) and proinflammatory cytokine production in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages and through the determination of scavenging activity. The results demonstrated that the ethanol extract from P. granatum and P. guajava leaves significantly inhibited NO production by suppressing nitric oxide synthase. The extracts also inhibited tumor necrosis factor-α, interleukin-1, and interleukin-6 in terms of both mRNA and protein levels and possessed high antioxidants. These extracts were shown to contain the highest amount of polyphenols. Our study concluded that among the plants studied, P. granatum and P. guajava have the most significant anti-inflammatory and antioxidant activities and polyphenols. These plants may have the potential for use in gastric ulcer therapy due to their indicated properties. Future research should focus on the isolation of their active compounds and their in vivo biological activities. Their beneficial applications need to be warranted by such evidence.

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Section: Collection and Preparation Of The Extractssupporting

confidence: 82%

Anti-Inflammatory and Antioxidant Activities of Medicinal Plants Used by Traditional Healers for Antiulcer Treatment

et al. 2019

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“…These results corresponded to the previous Ficus reports that showed bioactivities involving their lupeol profiles. [22][23][24] Although a direct correlation between the cytotoxic performance and lupeol content was observed in the extracted samples of F. racemosa and C. indicum, it was inverse in the extract of T. triandra, which possessed the highest cytotoxic potency in this study. The capability on cytotoxicity of T. triandra might correspond with prominent cytotoxic alkaloids, i.e.…”

Section: Discussioncontrasting

confidence: 55%

Determination of Lupeol, a Cytotoxic Compound Against SW620 Cells in the Extracts of Ha-Rak Recipe

Somwong¹,

Chuchote²

2021

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Introduction: Ha-Rak is an herbal recipe that has been stated by Ministry of Public Health, Thailand to utilize as the herbal medicines for the treatment of fever. The recipe comprises the roots of five plants including Capparis micracantha, Clerodendrum indicum, Ficus racemosa, Harrisonia perforata and Tiliacora triandra as its ingredients. Methods: The extracts of Ha-Rak and its five herbal principles were evaluated for their potential on the cytotoxicity against SW620 cancer cell lines using the MTT assay and an active compound, lupeol which was found as a cytotoxic compound in the herbal ingredients of Ha-Rak was further determined. A chromatographic method for investigating that compound in the extracts was developed herein. The analytical method comprised a cholesterol-coated C18 column, a mixture of acetonitrile and methanol (70:30) with a flow rate of 2 mL/min, and a diode-array UV wavelength at 210 nm as a stationary phase, mobile phase, and detector, respectively. Results: The amount of lupeol in the extracts of Ha-Rak and the herbal principles, which possessed cytotoxicity against SW620 cell lines with the IC 50 values of 30.10-212.24 µg/mL displayed variously in the range of 4.50-250.62 mg/100g. The difference of the contents among the extracts was noted significantly (P < 0.05). The correlation between the lupeol contents and their cytotoxic performances on the tested cell lines was observed in our investigation. Conclusion: Our findings demonstrated that Ha-Rak recipe contains a promising cytotoxic compound, lupeol and it will support the recipe to subject further pharmacological studies on anticancer activity.

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“…SWA was performed as described previously [ 27 ]. Briefly, cells (2 × 10 4 ) were incubated at 37 °C for 24 h. A scratch (wound) was performed on monolayer of cells along the vertical axis of each well under a light microscope.…”

Section: Methodsmentioning

confidence: 99%

Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo

Chen

You

Wang

et al. 2018

J Exp Clin Cancer Res

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BackgroundExosomes derived from tumor cells (TEXs) are involved in both immune suppression, angiogenesis, metastasis and anticancer stimulatory, but the biological characteristics and role of diffuse large B cell lymphoma (DLBCL)-derived exosomes have been less investigated.MethodsExosomes (EXOs) were isolated from OCI-LY3, SU-DHL-16, and Raji cells and biological characteristics of EXOs were investigated using electron microscopy, flow cytometry analysis, and Western blot analysis. The protein expression of EXOs was determined by an antibody array. Next, the communication between EXOs and lymphoma cell, stromal cell, dendritic cells (DCs), and T cells was evaluated. Finally, effect of DLBCL TEXs on tumor growth in vivo was investigated.ResultsWe demonstrated that EXOs derived from DLBCL cell lines displayed malignancy molecules such as c-Myc, Bcl-2, Mcl-1, CD19, and CD20. There was a different protein expression pattern between DLBCL TEXs and Burkitt lymphoma TEXs. DLBCL TEXs were easily captured by DCs and lymphoma cells, and mainly acted as an immunosuppressive mediator, evidenced by induction of apoptosis and upregulation of PD-1 in T cells. Furthermore, the TEXs stimulated not only cell proliferation, migration of stromal cells but also angiogenesis. As a result, the TEXs promoted tumor growth in vivo. On other hand, DLBCL TEXs did not induce apoptosis of DCs. After pulsed with the TEXs, DCs could stimulate clonal expansion of T cells, increase the secretion of IL-6 and TNFα, and decrease the production of immunosuppressive cytokine IL-4 and IL-10. The T cells from tumor bearing mice immunized by TEX were shown to possess superior antilymphoma potency relative to immunization of tumor lysates.ConclusionsThis study provides the framework for novel immunotherapies targeting TEXs in DLBCL.Electronic supplementary materialThe online version of this article (10.1186/s13046-018-0863-7) contains supplementary material, which is available to authorized users.

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Dual function of active constituents from bark of Ficus racemosa L in wound healing

Cited by 27 publications

References 31 publications

Anti-Inflammatory and Antioxidant Activities of Medicinal Plants Used by Traditional Healers for Antiulcer Treatment

Anti-Inflammatory and Antioxidant Activities of Medicinal Plants Used by Traditional Healers for Antiulcer Treatment

Determination of Lupeol, a Cytotoxic Compound Against SW620 Cells in the Extracts of Ha-Rak Recipe

Dual effect of DLBCL-derived EXOs in lymphoma to improve DC vaccine efficacy in vitro while favor tumorgenesis in vivo

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