Dual-functional 3D-printed composite scaffold for inhibiting bacterial infection and promoting bone regeneration in infected bone defect models

Yang, Ying; Chu, Linyang; Yang, Shengbing; Zhang, Hongbo; Qin, Ling; Guillaume, Olivier; Eglin, David; Richards, R. Geoff; Tang, Tingting

doi:10.1016/j.actbio.2018.08.015

Cited by 163 publications

(129 citation statements)

References 41 publications

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“…In the present study, we designed a Mg-incorporated scaffold with PLGA as the matrix using a low-temperature rapid-prototyping technique to repair small criticalsized bone defects with antibacterial activity. It has been reported that the addition of Mg particles to the PLGA scaffolds would increase the compressive strength and modulus compared with PLGA-only scaffolds [18,19]. In our study, the compression moduli of the PM-H and PM-L scaffolds increased by 134.2% and 86.3%, respectively, compared to the PLGA-only scaffolds (Fig.…”

Section: Discussionsupporting

confidence: 53%

In vitro antibacterial activity and cytocompatibility of magnesium-incorporated poly(lactide-co-glycolic acid) scaffolds

Wang

Yang

et al. 2020

BioMed Eng OnLine

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Background: Bone defects are often combined with the risk of infection in the clinic, and artificial bone substitutes are often implanted to repair the defective bone. However, the implant materials are carriers for bacterial growth, and biofilm can form on the implant surface, which is difficult to eliminate using antibiotics and the host immune system. Magnesium (Mg) was previously reported to possess antibacterial potential. Methods:In this study, Mg was incorporated into poly(lactide-co-glycolic acid) (PLGA) to fabricate a PLGA/Mg scaffold using a low-temperature rapid-prototyping technique. All scaffolds were divided into three groups: PLGA (P), PLGA/10 wt% Mg with low Mg content (PM-L) and PLGA/20 wt% Mg with high Mg content (PM-H). The degradation test of the scaffolds was conducted by immersing them into the trihydroxymethyl aminomethane-hydrochloric acid (Tris-HCl) buffer solution and measuring the change of pH values and concentrations of Mg ions. The antibacterial activity of the scaffolds was investigated by the spread plate method, tissue culture plate method, scanning electron microscopy and confocal laser scanning microscopy. Additionally, the cell attachment and proliferation of the scaffolds were evaluated by the cell counting kit-8 (CCK-8) assay using MC3T3-E1 cells. Results:The Mg-incorporated scaffolds degraded and released Mg ions and caused an increase in the pH value. Both PM-L and PM-H inhibited bacterial growth and biofilm formation, and PM-H exhibited higher antibacterial activity than PM-L after incubation for 24 and 48 h. Cell tests revealed that PM-H exerted a suppressive effect on cell attachment and proliferation. Conclusions:These findings demonstrated that the PLGA/Mg scaffolds possessed favorable antibacterial activity, and a higher content of Mg (20%) exhibited higher antibacterial activity and inhibitory effects on cell attachment and proliferation than low Mg content (10%).

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Section: Discussionsupporting

confidence: 53%

In vitro antibacterial activity and cytocompatibility of magnesium-incorporated poly(lactide-co-glycolic acid) scaffolds

Wang

Yang

et al. 2020

BioMed Eng OnLine

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“…The addition of HA induced an improvement of mechanical properties as demonstrated by the CS = 30 MPa and E = 1.9 GPa, which appeared to be intermediate between mechanical properties of the cancellous and cortical bones . These results demonstrated that this approach allowed to design and manufacture 3D‐printed scaffolds as bone substitutes with mechanical properties reassembling closely those of bone tissues, which could be applied in vivo for cortical and cancellous bone repair . The authors also proved that the use of quaternized chitosan decreased bacterial adhesion and biofilm formation under in vitro and in vivo conditions, disrupting microbial membranes and inhibiting the biofilm formation.…”

Section: Hard Matrix‐based Compositesmentioning

confidence: 79%

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Milazzo

Negrini

Scialla

et al. 2019

Adv Funct Materials

140

113

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Additive manufacturing (AM) techniques have gained interest in the tissue engineering field, thanks to their versatility and unique possibilities of producing constructs with complex macroscopic geometries and defined patterns. Recently, composite materials-namely, heterogeneous biomaterials identified as continuous phase (matrix) and reinforcement (filler)-have been proposed as inks that can be processed by AM to obtain scaffolds with improved biomimetic and bioactive properties. Significant efforts have been dedicated to hydroxyapatite (HA)-reinforced composites, especially targeting bone tissue engineering, thanks to the chemical similarities of HA with respect to mineral components of native mineralized tissues. Herein, applications of AM techniques to process HA-reinforced composites and biocomposites for the production of scaffolds with biological matrices, including cellular tissues, are reviewed. The primary outcomes of recent investigations in terms of morphological, structural, and in vitro and in vivo biological properties of the materials are discussed. The approaches based on the nature of the matrices employed to embed the HA reinforcements and produce the tissue substitutes are classified, and a critical discussion is provided on the presented state of the art as well as the future perspectives, to offer a comprehensive picture of the strategies investigated as well as challenges in this emerging field of materiomics.

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“…Female New Zealand white rabbits (3.0 ± 0.8 kg, Animal Center of Chongqing Medical University) were used to establish an infected femoral metaphysis model . Cylinder‐shaped bony defect (2.8 mm × 8 mm) perpendicular to the femoral metaphysis was created under sterile conditions according to the previous method . Eighteen rabbits were assigned to three groups randomly, namely, control Ti implants ( n = 6), AH‐Sr‐AgNPs implants ( n = 6), and AH‐Sr‐AgNPs implants locally injected with AP20187 ( n = 6, abbreviated as AH‐Sr‐AgNPs+AP20187).…”

Section: Methodsmentioning

confidence: 99%

Effects of Programmed Local Delivery from a Micro/Nano‐Hierarchical Surface on Titanium Implant on Infection Clearance and Osteogenic Induction in an Infected Bone Defect

Shrestha

et al. 2019

Adv Healthcare Materials

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The two major causes for implant failure are postoperative infection and poor osteogenesis. Initial period of osteointegration is regulated by immunocytes and osteogenic‐related cells resulting in inflammatory response and tissue healing. The healing phase can be influenced by various environmental factors and biological cascade effect. To synthetically orchestrate bone‐promoting factors on biomaterial surface, built is a dual delivery system coated on a titanium surface (abbreviated as AH‐Sr‐AgNPs). The results show that this programmed delivery system can release Ag+ and Sr2+ in a temporal‐spatial manner to clear pathogens and activate preosteoblast differentiation partially through manipulating the polarization of macrophages. Both in vitro and in vivo assays show that AH‐Sr‐AgNPs‐modified surface renders a microenvironment adverse for bacterial survival and favorable for macrophage polarization (M2), which further promotes the differentiation of preosteoblasts. Infected New Zealand rabbit femoral metaphysis defect model is used to confirm the osteogenic property of AH‐Sr‐AgNPs implants through micro‐CT, histological, and histomorphometric analyses. These findings demonstrate that the programmed surface with dual delivery of Sr2+ and Ag+ has the potential of achieving an enhanced osteogenic outcome through favorable immunoregulation.

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Dual-functional 3D-printed composite scaffold for inhibiting bacterial infection and promoting bone regeneration in infected bone defect models

Cited by 163 publications

References 41 publications

In vitro antibacterial activity and cytocompatibility of magnesium-incorporated poly(lactide-co-glycolic acid) scaffolds

In vitro antibacterial activity and cytocompatibility of magnesium-incorporated poly(lactide-co-glycolic acid) scaffolds

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Effects of Programmed Local Delivery from a Micro/Nano‐Hierarchical Surface on Titanium Implant on Infection Clearance and Osteogenic Induction in an Infected Bone Defect

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