Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration

Naskar, Deboki; Ghosh, Ananta K.; Mandal, Mahitosh; Das, Piyali; Nandi, Samit Kumar; Kundu, Subhas C.

doi:10.1016/j.biomaterials.2017.05.014

Cited by 131 publications

(78 citation statements)

References 65 publications

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“…The results indicated that the anticipated localized osteogenic and chondrogenic responses were achieved through the introduction of mechanical gradients in the hydrogels. The changes of osteogenesis and chondrogenesis were consistent with that happened on the osteochondral interface in vivo, superior to most of previous osteochondral tissue engineering systems fabricated through complex processes (Naskar et al, 2017;Zhang et al, 2017;Studle et al, 2018;Yang et al, 2018).…”

Section: Perpendicular Parallelsupporting

confidence: 76%

“…Native tissues such as skin, bone, nerve and muscle have multiple gradients to regulate cell behaviors and guide tissue functions (Lu and Thomopoulos, 2013;Vedadghavami et al, 2017;Di Donato et al, 2018;Li et al, 2018;Radhakrishnan et al, 2018;Wu et al, 2018). Both biochemical and physical cues including compositions, growth factors, stiffness and topography gradients play critical rules in controlling cell fate and tissue function (Oh et al, 2016;Naskar et al, 2017;Hubka et al, 2019). Strategies were developed to endow the engineered tissues with these different gradients in vitro for achieving functional recovery of damaged tissues (Han et al, 2016;Pogoda et al, 2017;Kokkinis et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Electric field-driven building blocks for introducing multiple gradients to hydrogels

Ding

Lü

et al. 2020

Protein Cell

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Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues. The introduction of gradient cues usually needs special equipment and complex process but is only effective to limited biomaterials. Incorporation of multiple gradients in the hydrogels remains challenges. Here, betasheet rich silk nanofibers (BSNF) were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric field. The blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems, finally achieving gradient distribution of the blocks in the formed hydrogels. The gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity, which resulted in highly tunable gradient of mechanical cues. The blocks were also aligned under the electric field, endowing orientation gradient simultaneously. Different cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field strategy. The building blocks could be introduced to various hydrogels such as Gelatin and NIPAM, indicating the universality. Complex niches with multiple gradient cues could be achieved through the strategy. Silk-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and chondrogenesis. Chondrogenic-osteogenic gradient transition was obtained, which stimulated the ectopic osteochondral tissue regeneration in vivo. The versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.

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Section: Perpendicular Parallelsupporting

confidence: 76%

Section: Introductionmentioning

confidence: 99%

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Ding

Lü

et al. 2020

Protein Cell

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“…Recently, with the development of emerging industries, nanofiber sponges generated by nanofiber interlocked with each other, have become promising candidates in the area of filtration, catalyst support, thermal insulators, energy storage, bone scaffolds and the like. As is well‐known, one of the most widely applied materials in the above area are light‐weight ceramic foams.…”

Section: Introductionmentioning

confidence: 99%

Novel micro‐spherical Si₃N₄ nanowire sponges from carbon‐doped silica sol foams via reverse templating method

et al. 2018

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A novel kind of nanowire sponges, namely Si3N4 nanowire‐weaving microspheres, synthesized from a simple, convenient, high‐efficient approach are proposed here. As the reverse template, three‐dimensional foam skeleton structure with uniform pores and ultrathin pore walls is constructed via the effective particle‐stabilized foam method, where the silica sol and carbon black are chosen as the raw materials, providing the sufficient space for the growth of nanowires during the carbothermal reduction reaction process. The formation mechanism of this novel sponge is studied via multiple characterization methods. Si3N4 nanowires formed microspheres possess uniform and curving morphology due to the stable environment for growing via vapor–solid mechanism, leading to the relatively high specific surface area of 86.77 m2/g. Owing to in‐situ oxidation process, micro‐spherical SiO2 nanowire sponges with similar morphology are synthesized, which present diameter in range of 20‐40 nm and specific surface area of 50.47 m2/g. This work provides insights for the design of high‐performance nanowire sponges with promising applications in the filtration, thermal insulators, and catalyst supports fields.

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“…Bone marrow stem cells (BMSCs) are multipotent cells with a high capacity for self‐renewal and differentiation potential into several cell types, including bone (osteoblasts), cartilage (chondrocytes), and fat (adipocytes) . In many cases of bone tissue engineering application, it is possible and highly regarded to make BMSCs differentiate toward a specific cell phenotype to generate new bone tissue by incorporating some osteoinductive or osteoconductive factors . Therefore, enhancing the functionality of scaffolds is extremely significant by loading bioactive substances into them to induce differentiation of BMSCs toward a specific phenotype in addition to serving as a temporary extracellular matrix to accommodate the regenerative of bone tissue .…”

Section: Introductionmentioning

confidence: 99%

Sustained release of dexamethasone from drug‐loadingPLGAscaffolds with specific pore structure fabricated by supercritical CO₂foaming

Xin

Guan

Yao

2018

J of Applied Polymer Sci

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Inducing differentiation of bone marrow stem cells to generate new bone tissue is highly desirable by controlling the release of some osteoinductive or osteoconductive factors from porous scaffolds. In this study, dexamethasone was selected as a representative of small molecule drugs and dexamethasone-loading porous poly(lactide-co-glycolide) (PLGA) scaffolds were successfully fabricated by supercritical CO 2 foaming. Scanning electron microscopy images showed that scaffolds had rough and relatively interconnected pores facilitating cells adhesion and growth. Specially, dexamethasone which was incorporated into PLGA matrix in a molecularly dispersed state could serve as a nucleation agent to be helpful for the formation of interconnected pores. Dexamethasone-loading porous PLGA scaffolds exhibited sustained release profile, and the delivery of dexamethasone from porous scaffolds could last for up to 2 months. The cumulative released amount of dexamethasone was relevant with drug loading capacity (1.66%-2.95%) and pore structure of scaffolds; while the release behavior was anomalous (non-Fickian) transport by fitting with the simple exponential equation, which had a diffusional exponent n higher than 0.5. It is feasible to fabricate drug-loading porous scaffolds by supercritical CO 2 foaming with specific pore structure and sustained release profile, which can be well applied in bone tissue engineering.

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Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration

Cited by 131 publications

References 65 publications

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Novel micro‐spherical Si₃N₄ nanowire sponges from carbon‐doped silica sol foams via reverse templating method

Sustained release of dexamethasone from drug‐loadingPLGAscaffolds with specific pore structure fabricated by supercritical CO₂foaming

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Dual growth factor loaded nonmulberry silk fibroin/carbon nanofiber composite 3D scaffolds for in vitro and in vivo bone regeneration

Cited by 131 publications

References 65 publications

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Novel micro‐spherical Si3N4 nanowire sponges from carbon‐doped silica sol foams via reverse templating method

Sustained release of dexamethasone from drug‐loadingPLGAscaffolds with specific pore structure fabricated by supercritical CO2foaming

Contact Info

Product

Resources

About

Novel micro‐spherical Si₃N₄ nanowire sponges from carbon‐doped silica sol foams via reverse templating method

Sustained release of dexamethasone from drug‐loadingPLGAscaffolds with specific pore structure fabricated by supercritical CO₂foaming