“…A central part of this research is development and application of new state-of-the-art bioanalytical techniques for advanced chemical and pharmacological profiling of complex extracts. This includes highresolution polypharmacological inhibition profiling (Zhao et al, 2018(Zhao et al, , 2019bLi et al, 2019;Ardalani et al, 2021;Liang et al, 2021) and ligand fishing (Wubshet et al, 2015;Petersen et al, 2019) for identification of bioactive constituents in crude extracts -followed by high-performance, liquid chromatography, photodiode array detection, high-resolution mass spectrometry, solid phase extraction and nuclear magnetic resonance spectroscopy (HPLC-PDA-HRMS-SPE-NMR) analysis for full structural identification of individual constituents (Lima et al, 2017;Zhao et al, 2018). Similarly, bioactivity-correlated metabolomics and bioaffinity NMR are techniques regularly employed to study bioactive natural products (Liu et al, 2017;Broholm et al, 2019).…”