Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3

Lindroth, Anders M.; Shultis, David; Jasencakova, Zuzana; Fuchs, Joerg; Johnson, Lianna M.; Schubert, Daniel; Patnaik, Debasis; Pradhan, Sriharsa; Goodrich, Justin; Schubert, Ingo; Jenuwein, Thomas; Khorasanizadeh, Sepideh; Jacobsen, Steven E.

doi:10.1038/emboj.2011.130

Cited by 20 publications

(29 citation statements)

References 31 publications

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“…Consistent with previous results in Arabidopsis and maize (Lindroth et al 2004;Zhang et al 2007;Makarevitch et al 2013), H3K27me3 regions were more abundant in euchromatic zones of chromosomes with a higher gene density and, apparently, less prevalent in the pericentromeric regions (Fig. 2).…”

Section: H3k27me3 Is Enriched In Gene Regionssupporting

confidence: 92%

Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach

Fuente

Conesa

Lloret

et al. 2015

Tree Genetics & Genomes

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Bud dormancy is an evolutionary adaptation of perennial plants to the seasonal fluctuation of temperatures in temperate climates, affected by intrinsic and environmental signals. Recent investigations point to a relevant role of epigenetic mechanisms in the regulation of bud dormancy. We have performed a chromatin immunoprecipitation sequencing (ChIP-seq) analysis of histone H3 lysine-27 trimethylation (H3K27me3), a chromatin mark associated with stable gene silencing, in dormant (D) and dormancy-released (ND) buds of peach (Prunus persica). H3K27me3 regions were more abundant in gene-rich euchromatic zones of chromosomes and associated with gene bodies. The dormancy regulators DORMANCY-ASSOCIATED MADS-box (DAM)1, DAM4, DAM5 and DAM6 were found significantly enriched in H3K27me3 in ND samples, in close agreement with their dormancy-specific expression. The DAM locus was modified at specific short regions, allowing the uneven regulation of distinct DAM genes. Additional regulatory factors related to meristem activity and flowering genes from Arabidopsis thaliana were differentially H3K27 trimethylated, which suggests that meristem reactivation and flower development could be also epigenetically regulated in reproductive buds of peach. A (GA)n motif and CACTA-type transposon-related sequences were found over-represented in H3K27me3 regions.

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Section: H3k27me3 Is Enriched In Gene Regionssupporting

confidence: 92%

Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach

Fuente

Conesa

Lloret

et al. 2015

Tree Genetics & Genomes

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“…Mull et al (2006) further proposed that the sensitivity of CHG methylation to SAH-hydrolase impairment may be explained by requirement of coordinate action of multiple transmethylation reactions. Indeed, the catalysis of CHG methylation is a complex process dependent on coordinate action of specific class of DNA methyltransferases (CMT3) and simultaneous methylation of lysine residues (K9, K27) in H3 histone (Jackson et al 2002;Malagnac et al 2002;Lindroth et al 2004). Recent observation of a hypomethylation effect of DHPA on H3K9 methylation (Baubec et al 2010) supports the hypothesis.…”

Section: Heritable Changes In Dna Methylation After the Sah-hydrolasementioning

confidence: 77%

Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco

et al. 2011

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Developmental processes are closely connected to certain states of epigenetic information which, among others, rely on methylation of chromatin. S-adenosylmethionine (SAM) and S-adenosylhomocysteine (SAH) are key cofactors of enzymes catalyzing DNA and histone methylation. To study the consequences of altered SAH/SAM levels on plant development we applied 9-(S)-(2,3-dihydroxypropyl)-adenine (DHPA), an inhibitor of SAH-hydrolase, on tobacco seeds during a short phase of germination period (6 days). The transient drug treatment induced: (1) dosage-dependent global DNA hypomethylation mitotically transmitted to adult plants; (2) pleiotropic developmental defects including decreased apical dominance, altered leaf and flower symmetry, flower whorl malformations and reduced fertility; (3) dramatic upregulation of floral organ identity genes NTDEF, NTGLO and NAG1 in leaves. We conclude that temporal SAH-hydrolase inhibition deregulated floral genes expression probably via chromatin methylation changes. The data further show that plants might be particularly sensitive to accurate setting of SAH/SAM levels during critical developmental periods.

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“…Moreover, in both regions, clusters of H3K27me1 occur. It has been shown in vitro that CMT3 is attracted to heterochromatin by binding methylated H3K27 (Lindroth et al 2004). It has also been proposed that H3K9me1 alone is not sufficient for DNA methylation in Arabidopsis heterochromatin and additionally H3K9me2 is required (Jackson et al 2004).…”

Section: Discussionmentioning

confidence: 97%

Epigenetic profiling of heterochromatic satellite DNA

Zakrzewski¹,

Weißhaar

Fuchs

et al. 2011

Chromosoma

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Sugar beet (Beta vulgaris) chromosomes consist of large heterochromatic blocks in pericentromeric, centromeric, and intercalary regions comprised of two different highly abundant DNA satellite families. To investigate DNA methylation at single base resolution at heterochromatic regions, we applied a method for strand-specific bisulfite sequencing of more than 1,000 satellite monomers followed by statistical analyses. As a result, we uncovered diversity in the distribution of different methylation patterns in both satellite families. Heavily methylated CG and CHG (H=A, T, or C) sites occur more frequently in intercalary heterochromatin, while CHH sites, with the exception of CAA, are only sparsely methylated, in both intercalary and pericentromeric/centromeric heterochromatin. We show that the difference in DNA methylation intensity is correlated to unequal distribution of heterochromatic histone H3 methylation marks. While clusters of H3K9me2 were absent from pericentromeric heterochromatin and restricted only to intercalary heterochromatic regions, H3K9me1 and H3K27me1 were observed in all types of heterochromatin. By sequencing of a small RNA library consisting of 6.76 million small RNAs, we identified small interfering RNAs (siRNAs) of 24 nucleotides in size which originated from both strands of the satellite DNAs. We hypothesize an involvement of these siRNAs in the regulation of DNA and histone methylation for maintaining heterochromatin.

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Dual histone H3 methylation marks at lysines 9 and 27 required for interaction with CHROMOMETHYLASE3

Cited by 20 publications

References 31 publications

Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach

Genome-wide changes in histone H3 lysine 27 trimethylation associated with bud dormancy release in peach

Inhibition of SAH-hydrolase activity during seed germination leads to deregulation of flowering genes and altered flower morphology in tobacco

Epigenetic profiling of heterochromatic satellite DNA

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