Dual infections of tilapia parvovirus (TiPV) and tilapia lake virus (TiLV) in multiple tilapia farms: Their impacts, genetic diversity, viral tropism, and pathological effects

Piewbang, Chutchai; Tattiyapong, Puntanat; Khemthong, Matepiya; Lachroje, Sitthichok; Boonrungsiman, Suwimon; Kasantikul, Tanit; Surachetpong, Win; Techangamsuwan, Somporn

doi:10.1016/j.aquaculture.2022.737887

Cited by 22 publications

(35 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This phenomenon warrants further investigation. Similar to the previous reports (Liu et al, 2020; Piewbang et al, 2022), TiPV was detected in various organs and cell types, indicating widespread cellular tropism, and the capability of infecting tilapia systemically. This finding is interesting, as parvoviruses are reported to be radiomimetic, i.e.…”

Section: Discussionsupporting

confidence: 90%

“…TiPV was also found in a relatively high prevalence (23.1%–64.6%) of randomly collected samples without clinical signs in multiple geographical locations in China (Liu et al, 2020). Most recently, TiPV and TiLV co‐infections were found in the majority of disease cases in juvenile tilapia in Thailand (Yamkasem, Tattiyapong, & Surachetpong, 2021; Yamkasem, Tattiyapong, Gorgoglione, & Surachetpong, 2021; Piewbang et al, 2022). Despite the fact that the fish population in this study was infected with both TiPV and S. agalactiae , only a small proportion of mortality was observed after handling stress, implying that both pathogens were in the chronic phase of infection.…”

Section: Discussionmentioning

confidence: 99%

“…The majority of fatalities occurred as a result of complicated co‐infection between tilapia lake virus (TiLV) and TiPV, mainly in juveniles (10–30 g), with recorded cumulative mortality rates of 50%–75%. Lethargy, haemorrhage, cutaneous ulceration, exophthalmos and abnormal swimming were all observed in diseased fish (Yamkasem, Tattiyapong, & Surachetpong, 2021; Yamkasem, Tattiyapong, Gorgoglione, & Surachetpong, 2021; Piewbang et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Non‐specific histopathological changes reported previously included tissue degeneration/necrosis, inflammatory infiltration by lymphocytes and macrophages, increased numbers of melano‐macrophage centres, etc. (Du et al, 2019; Liu et al, 2020; Yamkasem, Tattiyapong, & Surachetpong, 2021; Yamkasem, Tattiyapong, Gorgoglione, & Surachetpong, 2021; Piewbang et al, 2022). The objectives of this study were (1) to identify the pathognomonic lesion and the landmark organ(s) for histopathological diagnosis of TiPV in diseased tilapia, and (2) to investigate tissue tropism and localization of TiPV in infected tissues from diseased fish.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Usefulness of the pancreas as a prime target for histopathological diagnosis of Tilapia parvovirus (TiPV) infection in Nile tilapia, Oreochromis niloticus

Dong

Sangpo

Dien

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

Tilapia parvovirus (TiPV) is an emerging virus reportedly associated with disease and mortality in farmed tilapia. Although previous descriptions of histopathological changes are available, the lesions reported in these are not pathognomonic. Here, we report Cowdry type A inclusion bodies (CAIB) in the pancreas as a diagnostic histopathological feature found in adult Nile tilapia naturally infected with TiPV. This type of inclusion body has been well-known as a histopathological landmark for the diagnosis of other parvoviral infections in shrimp and terrestrial species. Interestingly, this lesion could be exclusively observed in pancreatic acinar cells, both in the hepatopancreas and pancreatic tissue along the intestine. In situ hybridization (ISH) using a TiPV-specific probe revealed the intranuclear presence of TiPV DNA in multiple tissues, including the liver, pancreas, kidney, spleen, gills and the membrane of oocytes in the ovary. These findings suggest that although TiPV can replicate in several tissue types, CAIB manifest exclusively in pancreatic tissues. In addition to TiPV, most diseased fish were co-infected with Streptococcus agalactiae, and presented with multifocal granulomas secondary to this bacterial infection. Partial genome amplification of TiPV was successful and revealed high nucleotide identity (>99%) to previously reported isolates. In summary, this study highlights the usefulness of pancreatic tissue as a prime target for histopathological diagnosis of TiPV in diseased Nile tilapia. This pattern may be critical when determining the presence of TiPV infection in new geographic areas, where ancillary testing may not be available. TiPV pathogenesis in this landmark organ warrants further investigation.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Usefulness of the pancreas as a prime target for histopathological diagnosis of Tilapia parvovirus (TiPV) infection in Nile tilapia, Oreochromis niloticus

Dong

Sangpo

Dien

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…TiLV had been reported to have a concurrent infection with Aeromonas spp. in farmed tilapia [ 25 , 26 ] and more recently with a novel Tilapia parvovirus [ 27 ]. However, in a wild environment, as in this study, the correlation between the monthly isolation of bacteria and TiLV in both wild tilapia and wild tinfoil barb was insignificant.…”

Section: Discussionmentioning

confidence: 99%

Persistent detection of Tilapia lake virus in wild tilapia and tinfoil barbs

Abdullah¹,

Pazai²,

Ridzuan³

et al. 2022

Vet World

View full text Add to dashboard Cite

Background and Aim: One of the emerging viral diseases in freshwater fish is Tilapia lake virus (TiLV), which infects all stages of fish and results in mass mortalities. Previously, a TiLV case was detected in the wild environment in Malaysia that involved tilapia and tinfoil barb. Hence, this study aimed to determine the presence of TiLV in wild tilapia (Oreochromis niloticus) as well as tinfoil barbs (Barbonymus schwanenfeldii) at the similar lake after the initial outbreak in year 2017. Materials and Methods: Both fish species were sampled from this lake at a month interval for two years and subjected to TiLV detection using reverse transcriptase-polymerase chain reaction and cell culture isolation. Concurrently, bacterial isolation and water quality measurements were performed to deduce their correlation with TiLV occurrence. Other wild fish species and mollusk were also occasionally sampled during the fish inventory activity at this lake. The fish's weight, length, and associated clinical signs were noted throughout the entire study period. Results: Mortality was not observed throughout the whole study period, and results indicated a moderate to high prevalence of TiLV infection in both tilapia and tinfoil barbs. There was no correlation between TiLV infection with the isolation rate of opportunistic bacteria such as Aeromonas spp., Plesiomonas spp., and Edwardsiella spp. in the study site. At the same time, the Pearson correlation test revealed a moderate negative correlation between the water pH with the presence of TiLV (R=–0.4472; p<0.05) and a moderate positive correlation between the water iron content with the monthly detection of Aeromonas spp. in wild tilapia. This is contrary to tinfoil barbs, where there was a moderate negative correlation between the water iron content with the monthly isolation of Aeromonas spp. (R=–0.5190; p<0.05). Furthermore, isolation of TiLV on cell culture-induced viral invasion was resulted in the cytopathic effects. Conclusion: Our results suggest that the wild fish may harbor TiLV for an extended period following a massive die-off event in 2017 without any obvious clinical signs and mortality. The persistency of viruses in the wild may need continuous and effective control as well as prevention strategies.

show abstract

Red tilapia by‐product hydrolysates: A new nitrogen source for Bifidobacterium lactisHN019

Jiang

Chen

et al. 2023

JSFA Reports

View full text Add to dashboard Cite

Background Red tilapia by‐products possess ample protein and are either discarded or processed into low‐value products. The nitrogen source is the most expensive part of the microbial culture, so finding a cheap alternative can better promote the microbial economy. In this study, different combinations of enzymes were used to hydrolyze the by‐products of red tilapia, and its effects on the growth of Bifidobacterium lactis HN019 were investigated. Results The results showed that hydrolysate hydrolyzed by enzyme combination 4 (alcalase: neutrase: papain: flavorzyme = 1:1:2:1) (EC4) obtained the highest nitrogen recovery (53.63%) and >2000 Da peptide proportion (5.07%). Hydrolysate hydrolyzed by enzyme combination 2 (alcalase: neutrase: papain: flavorzyme = 2:1:1:1) (EC2) has less hydrophobic amino acids and could improve the growth rate in 10 h–14 h in 50% nitrogen source substitution but had worst viable count after 24 h cultivation. Conclusion These results indicated that red tilapia by‐product hydrolysate was an excellent nitrogen source substitution and suggested that the hydrophobic amino acids in the nitrogen source might be an essential factor affecting the growth of Bifidobacterium lactis HN019. This research enhanced the economic value of red tilapia by‐products, minimized the waste of aquatic resources, and provided directions for the utilization of red tilapia by‐products.

show abstract

Dual infections of tilapia parvovirus (TiPV) and tilapia lake virus (TiLV) in multiple tilapia farms: Their impacts, genetic diversity, viral tropism, and pathological effects

Cited by 22 publications

References 40 publications

Usefulness of the pancreas as a prime target for histopathological diagnosis of Tilapia parvovirus (TiPV) infection in Nile tilapia, Oreochromis niloticus

Usefulness of the pancreas as a prime target for histopathological diagnosis of Tilapia parvovirus (TiPV) infection in Nile tilapia, Oreochromis niloticus

Persistent detection of Tilapia lake virus in wild tilapia and tinfoil barbs

Red tilapia by‐product hydrolysates: A new nitrogen source for Bifidobacterium lactisHN019

Contact Info

Product

Resources

About