Dual-layer drug release system based on ureteral stents inhibits the formation of ureteral stricture

Cai, Zhiduan; Luo, Wei; Zhuang, Haoquan; Ren, Congling; Pan, Xiaolin; Xu, Yuyu; Wang, Haoran; Li, Xiezhao; Yuan, Yaoji; Zhu, Rui; Zhan, Xiaowei; Jin, Lin; Xu, Guibin

doi:10.1016/j.cej.2023.144596

Cited by 6 publications

(1 citation statement)

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“…Immunohistochemistry or WB was used to detect the protein expression levels of tissues or cells according to the protocols we previously reported. 15 , 16 Specific primary antibodies were shown in Appendix S1 . IHC images were captured using a scanner model Pannoramic MIDI and Case Viewer software (3DHISTECH Ltd.) and quantitatively analyzed for integrated optical density (IOD) using Image‐Pro Plus software (6.0, Media Cybernetics).…”

Section: Methodsmentioning

confidence: 99%

MFN2 suppresses the accumulation of lipid droplets and the progression of clear cell renal cell carcinoma

Cai,

Luo,

Wang

et al. 2024

Cancer Science

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Dissolving the lipid droplets in tissue section with alcohol during a hematoxylin and eosin (H&E) stain causes the tumor cells to appear like clear soap bubbles under a microscope, which is a key pathological feature of clear cell renal cell carcinoma (ccRCC). Mitochondrial dynamics have been reported to be closely associated with lipid metabolism and tumor development. However, the relationship between mitochondrial dynamics and lipid metabolism reprogramming in ccRCC remains to be further explored. We conducted bioinformatics analysis to identify key genes regulating mitochondrial dynamics differentially expressed between tumor and normal tissues and immunohistochemistry and Western blot to confirm. After the target was identified, we created stable ccRCC cell lines to test the impact of the target gene on mitochondrial morphology, tumorigenesis in culture cells and xenograft models, and profiles of lipid metabolism. It was found that mitofusin 2 (MFN2) was downregulated in ccRCC tissues and associated with poor prognosis in patients with ccRCC. MFN2 suppressed mitochondrial fragmentation, proliferation, migration, and invasion of ccRCC cells and growth of xenograft tumors. Furthermore, MFN2 impacted lipid metabolism and reduced the accumulation of lipid droplets in ccRCC cells. MFN2 suppressed disease progression and improved prognosis for patients with ccRCC possibly by interrupting cellular lipid metabolism and reducing accumulation of lipid droplets.

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Section: Methodsmentioning

confidence: 99%