Dual-lifetime luminescent probe for time-resolved ratiometric oxygen sensing and imaging

Yang, Jun Qiang; Dai, Peiling; Li, Meng; Tang, Man; Wu, Qi; Liu, Shujuan; Zhao, Qiang; Zhang, Kenneth Yin

doi:10.1039/d2dt00467d

Cited by 5 publications

(3 citation statements)

References 31 publications

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“…Zhao, Zhang, and co-workers have developed a lifetime-based polymeric probe (645) (Chart 288) for time-resolved ratiometric O 2 imaging. 1221 The probe is composed of a fluorescent phenoxazine derivative and a phosphorescent iridium(III) complex, both of which emit at ca. 550 nm in deaerated aqueous buffer but with lifetimes of ca.…”

Section: Oxygenmentioning

confidence: 99%

“…Notably, the use of time-resolved techniques allows the separation of multiple emission signals with varying emission lifetimes at the same wavelength, enabling the analysis of the individual emission spectrum of the luminophores. Zhao, Zhang, and co-workers have developed a lifetime-based polymeric probe ( 645 ) (Chart ) for time-resolved ratiometric O 2 imaging . The probe is composed of a fluorescent phenoxazine derivative and a phosphorescent iridium(III) complex, both of which emit at ca .…”

Section: Luminescent Transition Metal Complexes As Probes For Microen...mentioning

confidence: 99%

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Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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Luminescence imaging is a powerful and versatile technique for investigating cell physiology and pathology in living systems, making significant contributions to life science research and clinical diagnosis. In recent years, luminescent transition metal complexes have gained significant attention for diagnostic and therapeutic applications due to their unique photophysical and photochemical properties. In this Review, we provide a comprehensive overview of the recent development of luminescent transition metal complexes for bioimaging and biosensing applications, with a focus on transition metal centers with a d 6 , d 8 , and d 10 electronic configuration. We elucidate the structure−property relationships of luminescent transition metal complexes, exploring how their structural characteristics can be manipulated to control their biological behavior such as cellular uptake, localization, biocompatibility, pharmacokinetics, and biodistribution. Furthermore, we introduce the various design strategies that leverage the interesting photophysical properties of luminescent transition metal complexes for a wide variety of biological applications, including autofluorescence-free imaging, multimodal imaging, organelle imaging, biological sensing, microenvironment monitoring, bioorthogonal labeling, bacterial imaging, and cell viability assessment. Finally, we provide insights into the challenges and perspectives of luminescent transition metal complexes for bioimaging and biosensing applications, as well as their use in disease diagnosis and treatment evaluation.

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Section: Oxygenmentioning

confidence: 99%

Section: Luminescent Transition Metal Complexes As Probes For Microen...mentioning

confidence: 99%

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Lee,

2024

Chem. Rev.

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“…Room-temperature phosphorescence (RTP), with the advantage of large Stokes shift and long lifetime, has been developed for applications in many fields, − such as optical oxygen sensors, chemical sensing, − as well as organic light-emitting devices. It is hard to detect the phosphorescence from free-base porphyrins because the transition from the triplet state to the ground state (S 0 ) is forbidden in the absence of significant spin–orbit coupling (SOC). − The incorporation of heavy atoms into the porphyrins’ molecular structure could lead to changes in photophysical parameters due to the enhancement of intercombination transitions, which is generally known as the heavy atom effect (HAE). − When the heavy atom is part of the molecule, it is called internal, and when the heavy atom belongs to the environment, it is called external .…”

Section: Introductionmentioning

confidence: 99%

Insight into the Heavy Atom Effect Induced by Environmental Heavy Atoms for Gadolinium-Labeled Hematoporphyrin Monomethyl Ether

et al. 2023

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Environmental heavy atoms can further enhance the room temperature phosphorescence (RTP) emissions of gadolinium-labeled hematoporphyrin monomethyl ether (Gd-HMME) by way of the external heavy atom effect (HAE). However, the macroscopic phosphorescence intensity covered the intrinsic effect of the environmental heavy atoms. In this study, a method of separating the external HAE from the total is performed, and a quantity to describe the intrinsic nature of external HAE is defined. The environmental Gd3+ concentration evolution of the phosphorescent transition rate (k P) is obtained by correlated absorption, emission, and time-resolved spectroscopy. The k P increases linearly with environmental Gd3+ concentration, while the intercept k P0 coincides with that of the internal HAE. The slope κ could be calculated, and it is a quantity free of the Gd3+ concentration and only relies on the type of environmental heavy atoms. In addition, the environmental Lu3+ exhibits similar functionality to Gd3+ in external HAE. Environmental Pd2+ quenches the phosphorescence intensity macroscopically, while it enhances the HAE intrinsically. Our method provides an alternative insight into the intrinsic nature of environmental heavy atoms.

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Iridium(III)‐Incorporating Self‐Healing Polysiloxanes as Materials for Light‐Emitting Oxygen Sensors

Parshina,

Deriabin,

Kolesnikov

et al. 2024

Macromol. Rapid Commun.

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Polymer‐metal complexes (PMCs) based on poly(2,2′‐bipyridine‐4,4′‐dicarboxamide‐co‐polydimethylsiloxanes) with cyclometalated di(2‐phenylpyridinato‐C2,N')iridium(III) fragments and cross‐linked by Zn2+ (Zn[Ir]‐BipyPDMSs) or Ir3+ (Ir[Ir]‐BipyPDMSs) represent flexible, stretchable, phosphorescent, and self‐healing molecular oxygen sensors. PMCs provide strong phosphorescence at λem = 595–605 nm. Zn[Ir]‐BipyPDMS with PDMS chain length of Mn = 5000 has the highest quantum yield of 9.3% and is a molecular oxygen sensor at different O2 concentrations (0–100 vol%) compared to Ir[Ir]‐BipyPDMSs. A Stern−Volmer constant is determined for Zn[Ir]‐BipyPDMS as KSV = 0.014%−1, which is similar to the reported oxygen‐sensitive iridium(III) complexes. All synthesized PMCs exhibit high elongation at break (up to 1100%) and self‐healing efficiency (up to 99%).

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Dual-lifetime luminescent probe for time-resolved ratiometric oxygen sensing and imaging

Abstract: Fluorescent/phosphorescent dual-emissive polymers or hybrids consisting of both fluorophore and phosphor have been used as self-calibrating probes and imaging reagents for cellular molecular oxygen. Oxygen selectively quenches the phosphorescence and...

Cited by 5 publications

References 31 publications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Shining New Light on Biological Systems: Luminescent Transition Metal Complexes for Bioimaging and Biosensing Applications

Insight into the Heavy Atom Effect Induced by Environmental Heavy Atoms for Gadolinium-Labeled Hematoporphyrin Monomethyl Ether

Iridium(III)‐Incorporating Self‐Healing Polysiloxanes as Materials for Light‐Emitting Oxygen Sensors

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