Dual-modal Assessment for in Vivo Investigation of Neurovascular Characteristic of Cerebral Edema Induced by Lipopolysaccharide

Abstract

The pathological features of cerebral edema are complicated, but usually only intracranial pressure (ICP) is regarded as the most important indicator for monitoring cerebral edema. The research focused on investigating the neurovascular characteristic of the lipopolysaccharide (LPS)-induced cerebral edema model in rats by using simultaneous electrophysical and hemodynamic recording. The results showed that neurophysiology (firing rate (FR), interval histogram index (ISI), and the power spectrum of local field potential (LFPs power)) and hemodynamic response (oxygenated hemoglobin (HbO2), deoxyhemoglobin (HbR) and relative cerebral blood flow (CBF)) were linearly related, and the Pearson’s correlation coefficient was determined by the BBB integrity after LPS injection. Furtherly, the improvement of treatment after two agents were observed successfully through these neurophysiological and hemodynamic parameters. The optical-electrical joint method provided a technical solution for cerebral edema functional monitoring and anti-edema drug efficacy evaluation. Our findings revealed the neurovascular and BBB impact of cerebral edema and improved the limitation of in vivo pathological diagnosis of cerebral edema.