Dual modality reflection mode optical coherence and photoacoustic microscopy using an akinetic sensor

Haindl, Richard; Preißer, Stefan; Andreana, Marco; Rohringer, Wolfgang; Sturtzel, Caterina; Distel, Martin; Chen, Zhe; Rank, Elisabet; Fischer, Balthasar; Drexler, Wolfgang; Li, Mengyang

doi:10.1364/ol.42.004319

Cited by 27 publications

(16 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1. For PAM an akinetic optical sensor with a large imaging window (2 mm • 12 mm) is used to detect refractive index modulations caused by acoustic waves [28,29]. The implementation of the detector allows direct reflection mode imaging, making it suitable for easy integration of OCT.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae

Haindl

Deloria

Sturtzel

et al. 2020

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

We present a dual modality functional optical coherence tomography and photoacoustic microscopy (OCT-PAM) system. The photoacoustic modality employs an akinetic optical sensor with a large imaging window. This imaging window enables direct reflection mode operation, and a seamless integration of optical coherence tomography (OCT) as a second imaging modality. Functional extensions to the OCT-PAM system include Doppler OCT (DOCT) and spectroscopic PAM (sPAM). This functional and non-invasive imaging system is applied to image zebrafish larvae, demonstrating its capability to extract both morphological and hemodynamic parameters in vivo in small animals, which are essential and critical in preclinical imaging for physiological, pathophysiological and drug response studies.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Recently a multimodal optical coherence tomography and photoacoustic microscopy system (OCT-PAM) was introduced [28]. The system uses an optical akinetic sensor [29], which can be positioned on top of the sample for reflection mode imaging.…”

Section: Introductionmentioning

confidence: 99%

Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae

Haindl

Deloria

Sturtzel

et al. 2020

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…6. This picture results from a multimodal imaging microscope, which combines optical coherence and photoacoustic microscopy (OC-PAM), providing complementary information for imaging biological tissues [5].…”

Section: Photoacoustic Imagingmentioning

confidence: 99%

Listening to Ultrasound with a Laser

Preißer¹,

Fischer²,

Panzer³

2017

Optik & Photonik

Self Cite

View full text Add to dashboard Cite

Ultrasound is widely used in applications ranging from non‐destructive testing of materials to medical imaging or even surgical interventions. As diverse as these applications may seem, they have a common interest: a precise measurement of the ultrasonic signals. Exact quantification is necessary to ensure proper performance of equipment as well as, sometimes, human safety. A new highly sensitive and broadband ultrasound detector is presented to fulfill the diverging demands of various ultrasound applications. In contrast to conventional sensors, it listens to ultrasound with a laser.

show abstract

“…[2][3][4][5] In addition, multimodal imaging techniques to integrate OCM with other optical imaging modalities, such as fluorescence microscopy, photoacoustic microscopy, and so on, have also been reported to provide complementary features of biological specimens, allowing more detailed biological understanding. 6,7 In the development of OCM and OCT systems currently underway, one of the important goals is to realize a large imaging depth in tissue imaging. In tissue imaging with OCM/OCT using near-infrared (NIR) light, the imaging depth of OCM/ OCT is limited by light attenuation due to multiple light scattering and light absorption events by water inside the samples.…”

Section: Introductionmentioning

confidence: 99%

High-spatial-resolution deep tissue imaging with spectral-domain optical coherence microscopy in the 1700-nm spectral band

2019

View full text Add to dashboard Cite

We present three-dimensional (3-D) highresolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at halfmaximum) in the 1700-nm spectral band. By using lowcoherence interferometry with SC light and a confocal detection scheme, we realized lateral and axial resolutions of 3.4 and 3.8 μm in tissue (n ¼ 1.38), respectively. This is, to the best of our knowledge, the highest 3-D spatial resolution reported among those of Fourier-domain optical coherence imaging techniques in the 1700-nm spectral band. In our SD-OCM, to enhance the imaging depth, a full-range method was implemented, which suppressed the formation of a coherent ghost image and allowed us to set the zero-delay position inside the samples. We demonstrated the 3-D high-resolution imaging capability of 1700-nm SD-OCM through the measurement of an interference signal from a mirror surface and imaging of a single 200-nm polystyrene bead and a pig thyroid gland. Deep tissue imaging at a depth of up to 1.8 mm was also demonstrated. This is the first demonstration of 3-D high-resolution SD-OCM in the 1700-nm spectral band.

show abstract

Dual modality reflection mode optical coherence and photoacoustic microscopy using an akinetic sensor

Cited by 27 publications

References 30 publications

Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae

Functional optical coherence tomography and photoacoustic microscopy imaging for zebrafish larvae

Listening to Ultrasound with a Laser

High-spatial-resolution deep tissue imaging with spectral-domain optical coherence microscopy in the 1700-nm spectral band

Contact Info

Product

Resources

About