Dual-mode colorimetric-photothermal sensing platform of acetylcholinesterase activity based on the peroxidase-like activity of Fe–N–C nanozyme

Lu, Liling; Hu, Xuehan; Zeng, Ruijin; Lin, Qianyun; Huang, Xue; Li, Meijin; Tang, Dianping

doi:10.1016/j.aca.2022.340383

Cited by 57 publications

(14 citation statements)

References 47 publications

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“…To assess the oxidase activity of Ru@g-C 3 N 4 , TMB was used as an indicator by observing the conversion of colorless TMB to blue oxTMB with absorption at a wavelength of 655 nm. , As shown in Figure A,B, the visual color and the absorption intensity at 655 nm were significantly changed in the Ru@g-C 3 N 4 and TMB reaction systems, and no such changes were observed in the other groups. Meanwhile, by using 5,5-dimethyl-1-pyrroline-N-oxide (DMPO) as a free radical scavenger in the electron paramagnetic resonance (EPR) study, clear signs of superoxide radicals (O 2 •– ) were detected (Figure C).…”

Section: Resultsmentioning

confidence: 99%

Blocking Oxidase-Mimicking Activity-Driven Label-Free Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Detection of Tetracycline Residues in Milk

Liu,

Wu,

Nie

et al. 2024

ACS Food Sci. Technol.

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Considering the potential misuse of antibiotics to improve the yield and economic benefits in the production of animal-derived foods such as milk, the development of a simple, label-free, precise, reliable, low-cost, and onsite assay for antibiotic residues is highly desirable to ensure food safety. In this scenario, we here proposed a hybrid oxidase-mimicking nanozyme of the Ru@g-C 3 N 4 nanosheet that combines with 3,3′,5,5′-tetramethylbenzidine (TMB) to develop a blocking oxidase-mimicking activitydriven photothermal and colorimetric synergistic sensing platform for intelligent onsite detection of tetracycline antibiotics (TCs). In the absence of TCs, the prominent oxidase-mimicking activity of Ru@g-C 3 N 4 rapidly catalyzed colorless TMB to dark-blue oxTMB, accompanied by the appearance of an obvious absorbance enhancement at 655 nm and a remarkable photothermal signal upon 660 nm laser irradiation. Conversely, the remaining TCs effectively blocked this chromogenic reaction because of the π−π stacking of its tetraphenyl skeleton with the Ru@g-C 3 N 4 nanozyme to block the affinity of TMB to the catalytic active site inside Ru@g-C 3 N 4 . Based on this, a TCs residual level-dependent photothermal and colorimetric signal synergistic decrease sensing platform was developed with the assistance of a 660 nm laser-assisted hand-held thermal imager and a Color Picker APP-inbuilt smartphone, which demonstrated the intelligent onsite label-free, reliable, and precise detection of TCs in real milk samples with acceptable results. Notably, this oxidase-mimicking nanozyme-based dual-mode sensing platform provided a built-in cross-reference correction to improve not only the accuracy but also the reliability for TC detection in milk, which showed a vast promise in the further development of commercial point-of-care testing to achieve early warning of antibiotic residues to ensure the quality and safety of animal-derived foodstuffs.

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Section: Resultsmentioning

confidence: 99%

Blocking Oxidase-Mimicking Activity-Driven Label-Free Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Detection of Tetracycline Residues in Milk

Liu,

Wu,

Nie

et al. 2024

ACS Food Sci. Technol.

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“…268 Besides, as oxTMB also exhibits photothermal capacity when exposed to an 808 nm laser, the activity of AChE can be determined from the temperature signal using a near-infrared imaging camera within a minute. 218 However, the Fe–N–C SACs are still limited by their biocatalytic activity and selectivity. A boron-doped Fe–N–C SAC with FeN 4 –2B coordination environments can detect AChE in the range of 0.8–100 mU (Fig.…”

Section: Sensing Applications Of Sacsmentioning

confidence: 99%

Single-atom catalysts: promotors of highly sensitive and selective sensors

Tian

Wang

et al. 2023

Chem. Soc. Rev.

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“…An easy-to-use colorimetric detection technique has attracted extensive attention by its simplicity, low cost, and sensitivity. − Many colorimetric sensing platforms have been developed for various applications. , Natural enzymes have the outstanding merits of high specificity and catalytic efficiency but the intrinsic limitations of poor stability, high purification cost, and short shelf-life, thus hindering their broad applications. − In the past few decades, researchers have developed some artificial mimics to replace natural enzymes to meet the demands of colorimetric assay. − Among them, nanozymes, a novel type of nanomaterial with enzyme-like activities, are superior based on the large-scale preparation, low price, satisfactory adaptability, unique physicochemical characteristics, and good catalytic activity. − Since Yan and co-workers reported the use of Fe 3 O 4 nanoparticles as peroxidase (POD) mimics in their pioneering work of 2007, various types of nanomaterials have been established as substitutes for natural enzymes.…”

Section: Introductionmentioning

confidence: 99%

Highly Active CoRh Graphitic Nanozyme for Colorimetric Sensing in Real Samples

Keoingthong

et al. 2023

J. Phys. Chem. B

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Rh-based nanozymes show high catalytic efficiency, specific surface area, good stability, and unique physicochemical properties, while magnetic nanozymes facilitate the magnetic separation of detection samples under an external magnetic field for improved sensitivity. However, magnetic Rh nanozymes, especially those with excellent stability, have not been reported. Herein, we apply the chemical vapor deposition (CVD) method to prepare a CoRh graphitic nanozyme (termed as CoRh@G nanozyme), which structurally consists of CoRh nanoalloy encapsulated by a few layers of graphene for sensitive colorimetric sensing applications. The proposed CoRh@G nanozyme has superior peroxidase (POD)-like activity, and it shows higher affinity of the CoRh@G nanozyme than horseradish peroxidase (HRP) toward 3,3′,5,5′-tetramethylbenzydine (TMB) oxidation. In addition, the CoRh@G nanozyme shows high durability and superior recyclability owing to its protective graphitic shell. The outstanding merits of the CoRh@G nanozyme allow its use for quantitative colorimetric detection of dopamine (DA) and ascorbic acid (AA), showing high sensitivity and good selectivity. Moreover, it shows satisfactory performance for AA detection in commercial beverages and energy drinks. The proposed CoRh@G nanozyme-based colorimetric sensing platform shows great promise in point-of-care (POC) visual monitoring.

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Dual-mode colorimetric-photothermal sensing platform of acetylcholinesterase activity based on the peroxidase-like activity of Fe–N–C nanozyme

Cited by 57 publications

References 47 publications

Blocking Oxidase-Mimicking Activity-Driven Label-Free Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Detection of Tetracycline Residues in Milk

Blocking Oxidase-Mimicking Activity-Driven Label-Free Photothermal and Colorimetric Synergistic Sensing for Intelligent Onsite Detection of Tetracycline Residues in Milk

Single-atom catalysts: promotors of highly sensitive and selective sensors

Highly Active CoRh Graphitic Nanozyme for Colorimetric Sensing in Real Samples

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