Dual-model photoelectrochemical biosensor via DNAzyme walker integrated nanoprobe for ultrasensitive ratiometric detection of microRNA-155

Recent advances using MXenes in biomedical applications

“…In general, gold nanoparticles are incorporated, especially for the conjugation of DNA probes. 144–148 The sensing range for miRNA, 144–146 DNA/RNA, 147,149 or genes 148 is lower than ∼nM. For the sensing of proteins ( e.g.…”

“…168 In a signal amplification strategy, PtCo@Prussian blue nanozyme was selected as a signal nanoprobe with high catalytic activity and large specific surface area, while two dimensional MXene/AuNPs as a surface modification material was applied to improve the active sites of the electrode. 146 This miR-155 biosensor shows a low limit of detection of 1.2 fM and a sensing range of 5 fM to 5 nM. 146 Li et al developed an ECL biosensor based on bimetallic MXene derivative QDs (Mo 2 TiC 2 QDs) and SnS 2 nanosheets/lipid bilayers to detect the gastric cancer marker miRNA-27a-3p.…”

“…146 This miR-155 biosensor shows a low limit of detection of 1.2 fM and a sensing range of 5 fM to 5 nM. 146 Li et al developed an ECL biosensor based on bimetallic MXene derivative QDs (Mo 2 TiC 2 QDs) and SnS 2 nanosheets/lipid bilayers to detect the gastric cancer marker miRNA-27a-3p. 167 The inter-band excitation of Mo 2 TiC 2 QDs can inject additional carriers, which were less suppressed by boundary effects making a significant contribution to the luminescence process.…”

Self-Powered Biosensor Based on DNA Walkers for Ultrasensitive MicroRNA Detection

“…MicroRNAs (miRNAs) are noncoding single-stranded RNAs containing 21∼25 nucleotides, which have a crucial impact in the adjustment of gene representation at the translational level. − At present, a number of research results have indicated that the improper expression of miRNAs is closely related with the etiopathogenesis of many cancers. , For example, the maladjusted expression of miRNA Let-7a and miRNA-21 is intensely relevant with early breast cancer . The abnormal expression of miRNA-141 could be an indicator for the development of prostate cancer, and miRNA-155 is closely correlated with cervical cancer . Thus, the highly sensitive detection of miRNA is essential to the investigation of the growth and progression of cancers.…”

“…6 The abnormal expression of miRNA-141 could be an indicator for the development of prostate cancer, 7 and miRNA-155 is closely correlated with cervical cancer. 8 Thus, the highly sensitive detection of miRNA is essential to the investigation of the growth and progression of cancers. There are currently several methods to monitor miRNAs, 9−11 such as electrochemical luminescence, 12 photochemistry, 13 colorimetry, 14 and fluorescence.…”