Dual origin of melanocytes defined by <scp>S</scp>ox1 expression and their region‐specific distribution in mammalian skin

Yoshimura, Naoko; Motohashi, Tsutomu; Aoki, Hitomi; Tezuka, Ken Ichi; Watanabe, Natsuki; Wakaoka, Takanori; Era, Takumi; Kunisada, Takahiro

doi:10.1111/dgd.12034

Cited by 10 publications

(14 citation statements)

References 52 publications

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“…We recently showed that a certain skin melanocyte lineage cell population was marked by keratinocyte‐specific cell lineage tracking (Yoshimura et al . ) using the K14‐Cre allele (Hafner et al . ) in K14‐Cre; RosaR26R‐LacZ mice.…”

Section: Resultsmentioning

confidence: 99%

“…1I). We recently showed that a certain skin melanocyte lineage cell population was marked by keratinocyte-specific cell lineage tracking (Yoshimura et al 2013) using the K14-Cre allele (Hafner et al 2004) in K14-Cre; RosaR26R-LacZ mice. However, K14-Cre; Rest floxed /+ mice as well as K14-Cre; Rest floxed/floxed mice never developed white spots ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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White spotting phenotype induced by targeted REST disruption during neural crest specification to a melanocyte cell lineage

2015

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Neural crest cells (NCCs) emerge from the dorsal region of the neural tube of vertebrate embryos and have the pluripotency to differentiate into both neuronal and non-neuronal lineages including melanocytes. Rest, also known as NRSF (neuro-restrictive silencer factor), is a regulator of neuronal development and function and suggested to be involved in the lineage specification of NCCs. However, further investigations of Rest gene functions in vivo have been hampered by the fact that Rest null mice show early embryonic lethality. To investigate the function of Rest in NCC development, we recently established NCC-specific Rest conditional knockout (CKO) mice and observed their neonatal death. Here, we have established viable heterozygous NCC-specific Rest CKO mice to analyze the function of Rest in an NCC-derived melanocyte cell lineage and found that the white spotting phenotype was associated with the reduction in the number of melanoblasts in the embryonic skin. The Rest deletion induced after the specification to melanocytes did not reduce the number of melanoblasts; therefore, the expression of REST during the early neural crest specification stage was necessary for the normal development of melanoblasts to cover all of the skin.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

White spotting phenotype induced by targeted REST disruption during neural crest specification to a melanocyte cell lineage

2015

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“…Moreover, ascidian pigment cells could be reprogrammed into migrating mesenchyme‐like cells by the targeted misexpression of Twist, mostly to mimic vertebrate NCCs (Abiuta et al, ). The findings that a significant population of mouse skin melanocytes originates from embryonic epidermal skin and that this population never differentiates to another NCC lineage (Yoshimura et al, ) seem to support the hypothesis that NCCs are descendants of pigment cells of invertebrates. However, it should be noted that all of this evidence is circumstantial and needs to be further substantiated, for example by finding an ascidian species in which the bipotential pigment cells differentiate to neural cells during development.…”

Section: Introductionmentioning

confidence: 83%

The stemness of neural crest cells and their derivatives

Kunisada

Tezulka

Aoki

et al. 2014

Birth Defects Research Pt C

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Neural crest cells (NCCs) are unique to vertebrates and emerge from the border of the neural plate and subsequently migrate extensively throughout the embryo after which they differentiate into many types of cells. This multipotency is the main reason why NCCs are regarded as a versatile tool for stem cell biology and have been gathering attention for their potential use in stem cell based therapy. Multiple sets of networks comprised of signaling molecules and transcription factors regulate every developmental phase of NCCs, including maintenance of their multipotency. Pluripotent stem cell lines, such as embryonic stem cells and induced pluripotent stem (iPS) cells, facilitate the induction of NCCs in combination with sophisticated culture systems used for neural stem cells, although at present, clinical experiments for NCC-based cell therapy need to be improved. Unexpectedly, the multipotency of NCCs is maintained after they reach the target tissues as tissue neural crest stem cells (NCSCs) that may contribute to the establishment of NCC-derived multipotential stem cells. In addition, under specific culture conditions, fate-restricted unipotent descendants of NCCs, such as melanoblasts, show multipotency to differentiate into melanocytes, neurons, and glia cells. These properties contribute to the additional versatility of NCCs for therapeutic application and to better understand NCC development.

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“…Almost all colonies were Tomato-positive from E7.5- 3B). We further investigated whether neuroectoderm cells could contribute to adult BM-MSC using a Sox1-Cre knock-in mouse line as reported previously (Takashima et al, 2007;Yoshimura et al, 2013). Sox1 was shown to be the most specific marker for neuroepithelial cells (Fig.…”

Section: Destinies Of Mscs and Their Progenitors From Embryo To Adultmentioning

confidence: 91%

Tracing the destiny of mesenchymal stem cells from embryo to adult bone marrow and white adipose tissue via Pdgfrα expression

Miwa

Era

2018

Development

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Mesenchymal stem cells (MSCs) are somatic stem cells that can be derived from adult bone marrow (BM) and white adipose tissue (WAT), and that display multipotency and self-renewal capacity. Although MSCs are essential for tissue formation and have already been used in clinical therapy, the origins and markers of these cells remain unknown. In this study, we first investigated the developmental process of MSCs in mouse embryos using the gene encoding platelet-derived growth factor receptor α () as a marker. We then traced cells expressing and other genes (brachyury, and ) in various mutant mouse embryos until the adult stage. This tracing of MSC origins and destinies indicates that embryonic MSCs emerge in waves and that almost all adult BM MSCs and WAT MSCs originate from mesoderm and embryonic Pdgfrα-positive cells. Furthermore, we demonstrate that adult Pdgfrα-positive cells are involved in some pathological conditions.

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Dual origin of melanocytes defined by Sox1 expression and their region‐specific distribution in mammalian skin

Cited by 10 publications

References 52 publications

White spotting phenotype induced by targeted REST disruption during neural crest specification to a melanocyte cell lineage

White spotting phenotype induced by targeted REST disruption during neural crest specification to a melanocyte cell lineage

The stemness of neural crest cells and their derivatives

Tracing the destiny of mesenchymal stem cells from embryo to adult bone marrow and white adipose tissue via Pdgfrα expression

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