2014
DOI: 10.1186/1478-811x-12-5
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Dual Oxidase Maturation factor 1 (DUOXA1) overexpression increases reactive oxygen species production and inhibits murine muscle satellite cell differentiation

Abstract: BackgroundDual oxidase maturation factor 1 (DUOXA1) has been associated with the maturation of the reactive oxygen species (ROS) producing enzyme, dual oxidase 1 (DUOX1) in the adult thyroid. However, ROS have also been implicated in the development of several tissues. We found that activated muscle satellite cells and primary myoblasts isolated from mice express robust levels of DUOXA1 and that its levels are altered as cells differentiate.ResultsTo determine whether DUOXA1 levels affect muscle differentiatio… Show more

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Cited by 41 publications
(35 citation statements)
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“…48 In addition to regulating Numb, NIP/DUOXA1 also has an important role in, for example, myogenesis, with overexpression of NIP/DUOXA1 inhibiting differentiation and promoting apoptosis through enhanced DUOX1-dependent ROS production. 49 To address the potential involvement of DUOXA1, we used either direct siRNA silencing or overexpression of DUOXA1, which did not significantly affect EMT (Supplementary Figure S2C; Figure 5). Therefore, although some molecular alterations associated with DUOX1 silencing may have resulted from indirect changes in, for example, Notch/Numb status due to suppression of DUOXA1, the observed effects on EMT appear to be primarily due to loss of DUOX1 itself.…”
Section: Discussionmentioning
confidence: 99%
“…48 In addition to regulating Numb, NIP/DUOXA1 also has an important role in, for example, myogenesis, with overexpression of NIP/DUOXA1 inhibiting differentiation and promoting apoptosis through enhanced DUOX1-dependent ROS production. 49 To address the potential involvement of DUOXA1, we used either direct siRNA silencing or overexpression of DUOXA1, which did not significantly affect EMT (Supplementary Figure S2C; Figure 5). Therefore, although some molecular alterations associated with DUOX1 silencing may have resulted from indirect changes in, for example, Notch/Numb status due to suppression of DUOXA1, the observed effects on EMT appear to be primarily due to loss of DUOX1 itself.…”
Section: Discussionmentioning
confidence: 99%
“…Overexpression and inhibition of DUOXA1 in MuSCs reduces and enhances in vitro myogenic differentiation, respectively (293). In vivo, genetic deletion of the p66…”
Section: E Myogenic Differentiationmentioning
confidence: 99%
“…Beyond its role as a maturation factor for DUOX1, or in regulating the Notch pathway, DUOXA1 may also have other independent functional roles. For example, recent studies implicated DUOXA1 in cellular differentiation in muscle satellite cells[257] and neuronal cells[258, 259], which appears to involve interactions with p53, well-appreciated for its tumor suppressor function and its roles in cell cycle dynamics and differentiation[258]. Also, DUOXA1 was found to harbor roles in various tissues such as the brain[258, 259] and breast[260], which appear unrelated to DUOX1.…”
Section: Duox and Their Maturation Factors: Partners Or Enemies?mentioning
confidence: 99%