This paper describes a computational 3-D imaging system based on diffraction grating imaging with laser sources of multiple wavelengths. It was proven that a diffraction grating imaging system works well as a 3-D imaging system in our previous studies. The diffraction grating imaging system has advantages such as no spherical aberration and a low-cost system, compared with the well-known 3-D imaging systems based on a lens array or a camera array. However, a diffraction grating imaging system still suffers from noises, artifacts, and blurring due to the diffraction nature and illumination of single wavelength lasers. In this paper, we propose a diffraction grating imaging system with multiple wavelengths to overcome these problems. The proposed imaging system can produce multiple volumes through multiple laser illuminators with different wavelengths. Integration of these volumes can reduce noises, artifacts, and blurring in grating imaging since the original signals of 3-D objects inside these volumes are integrated by our computational reconstruction method. To apply the multiple wavelength system to a diffraction grating imaging system efficiently, we analyze the effects on the system parameters such as spatial periods and parallax angles for different wavelengths. A computational 3-D imaging system based on the analysis is proposed to enhance the image quality in diffraction grating imaging. Optical experiments with three-wavelength lasers are conducted to evaluate the proposed system. The results indicate that our diffraction grating imaging system is superior to the existing method.