Dual-recognition membrane Adsorbers combining hydrophobic charge-induction chromatography with surface imprinting via multicomponent reaction

Guo, Miaoyuan; Ye, Jianlong; Zheng, Chunhong; Meng, Jianqiang

doi:10.1016/j.chroma.2022.462918

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…A membrane measuring 1 × 1 cm 2 was immersed in 1 mL of protein solution with varying concentrations of 0.5, 1, 1.5, 2, 3, 4, and 5 mg/mL. The incubation took place at a temperature of 30 °C for a duration of 22 h. Once equilibrium was achieved, the protein adsorption capacity was determined using eq , and then the results were fitted by Langmuir adsorption isotherm eq : Langmuir .25em model : C e Q e = 1 K d Q m + C e Q m where C e (mg/mL) is the equilibrium protein concentration, Q e (mg/g) is the protein uptake at C e , Q m (mg/g) is the protein saturated uptake, and K d is the dissociation constant.…”

Section: Methodsmentioning

confidence: 99%

“…A membrane measuring 1 × 1 cm 2 was immersed in 1 mL of protein solution with a concentration of 3 mg/mL. The protein adsorption capacity was determined using eq at the adsorption time of 2, 4, 6, 8, 10, 12, of 22 h, respectively, and the results were fitted by pseudo-first-order kinetic eq : pseudo ‐ first ‐ order ‐ model : 0.25em Q t = Q e ( 1 − 1 e k 1 t ) where Q e (mg/g) is the equilibrium protein uptake, Q t (mg/g) is the protein uptake of time t (h), and k 1 is the kinetic constant of pseudo-first-order.…”

Section: Methodsmentioning

confidence: 99%

“…The recovery rate of lysozyme was calculated according to eq : R false( % false) = elute 0.25em amount false( C e V e false) uptake 0.25em amount false( C 0 V 0 − C b t V b t false) × 100 % where C 0 , C bt , and C e represent the injected lysozyme concentration (mg/mL), the breakthrough lysozyme concentration (mg/mL), and the eluted lysozyme concentration (mg/mL), respectively. V 0 , V bt , and V e are the injected lysozyme volume (mL), lysozyme breakthrough volume (mL), and eluted lysozyme volume (mL), respectively.…”

Section: Methodsmentioning

confidence: 99%

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Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Peng,

Wu,

et al. 2024

ACS Appl. Nano Mater.

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Developing bioseparation media with both high strength and high surface area capable of efficiently separating proteins is a significant challenge. In this study, nanofibrous membranes (NFMs) with mechanical strength up to 10.8 MPa were successfully prepared by using a simple hot pressing and cross-linking process. Subsequently, mixed-mode chromatography (MMC) with both electrostatic and hydrophobic interactions was achieved by introducing glutaric anhydride (GA) onto the surface of the regenerated cellulose (RC) nanofibers. The separation of lysozyme using MMC membranes showed that the static adsorption of lysozyme (1 mg/mL) on RC-g-GA NFM reached 254 mg/g. The dynamic binding capacity of lysozyme in the flow-through mode at 10% breakthrough was 72.8 mg/g. Meanwhile, additionally, the RC-g-GA NFM could saturate lysozyme (3 mg/mL) adsorption up to 336 mg/g after 6 h of adsorption time. Moreover, the membrane could isolate lysozyme with 85% purity from a mixed solution of lysozyme and γ-globulin. This work demonstrates that RC-g-GA NFM has an efficient lysozyme isolation capability and potential for scale-up applications.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Peng,

Wu,

et al. 2024

ACS Appl. Nano Mater.

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“…Tryptamine and tryptophan, two hydrophobic charge-induction chromatographic ligands (a type of mixed mode), were used to develop dual-ligand resins. This type of ligand employs various interaction modes, including ionic exchange, hydrophobic interaction, hydrogen bonding, etc., − providing unique adsorption selectivity, high capacity, salt tolerance, and relatively low cost. − Both tryptamine and tryptophan consists of a six-membered benzene ring fused to a five-membered pyrrole ring, forming an indole structure. One of the carbon atoms on the pyrrole ring is attached to an amino group.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Recovery of Bovine Serum Albumin and Bovine Immunoglobulin G with Dual-Ligand Hydrophobic Charge-Induction Chromatography

Shi

Zhu

et al. 2023

Ind. Eng. Chem. Res.

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This study investigated the potential of dual-ligand hydrophobic charge-induction chromatography for the simultaneous recovery of two high-value components (bovine serum albumin and bovine immunoglobulin G). Compared to single-ligand chromatography, dual-ligand chromatography offers greater flexibility and room for improvement. Tryptophan and tryptamine were used as ligands to prepare a series of single- and dual-ligand resins. Bovine serum albumin and bovine immunoglobulin G were used for adsorption and comparison in batch, frontal adsorption, and recovery experiments. The results showed that the dual-ligand mode had a stable coupling efficiency ratio (tryptamine to tryptophan, ∼2.30) during preparation and could break through the upper limit of the adsorption capacity of single-ligand mode. Additionally, it exhibited good adsorption performance for both bovine serum albumin and bovine immunoglobulin G from bovine serum (recoveries of 90.73 and 101.4%, respectively). The findings of this study confirmed that dual-ligand chromatography can be used as an effective strategy for biological separation.

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“…Fluorescence spectrometry (Sitko et al 2011), infrared spectroscopy (IR) (Youli et Molecular imprinting technology (MIT) was a molecular recognition technology developed based on the simulation of antigen and antibody interactions in living organisms (Guo and Ye et al 2022). Due to the high selectivity, low cost and reusability of the prepared molecularly imprinted polymers (MIPs), MIT had become one of the important technology for sample extraction and separation (Ratautaite et al 2021).…”

mentioning

confidence: 99%

Dummy Template–Based Molecularly Imprinted Solid-Phase Microextraction Coating for Analysis of Plasticizers in Food Samples

Li,

Chen,

et al. 2024

Food Anal. Methods

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A series of molecularly imprinted polymers (MIPs) were prepared on the surface of microspheres(CG161M) and applied as adsorbent for the rapid and selective detection of phthalates compounds, including diethyl phthalate (DEP), dibutyl phthalate (DBP), dioctyl phthalate (DOP). Surface deposition and layer by layer self-assembling method were also utilized in the preparetion of molecularly imprinted polymers. The synthesized composites were characterized by Fourier transform infrared spectrometer, scanning electron microscope, thermo gravimetric analysis and Nitrogen adsorption analysis. The maximum adsorption capacities of the MIPs for DEP, DBP and DOP were 0.006, 0.008 and 0.007 mg g − 1 , respectively. The adsorption of phthalates reached equilibrium within 260 min and complied well with pseudo-second-order kinetic model and Langmuir model. Dioctyl phthalate(DOP) was used as a dummy template for diethyl phthalate(DEP) and dibutyl phthalate(DEP), allowing selective and speci c identi cation of DEP and DBP and did not affect the accuracy of the analysis even if the leakage of template occured. Moreover, MIPs-based hollow ber stir bar sorptive extraction followed by gas chromatography-mass spectrometry was applied to the detection of DEP, DBP and DOP in several food samples. Under the optimum conditions, the limits of detection (LODs) for DEP, DBP and DOP were 0.0047, 0.0054 and 0.0031 mg L − 1 , with spiked recoveries of 73.06-106.02% and relative standard deviations (RSDs) of 3.91-6.89%, exhibiting high adsorption capacity, good selectivity and fast kinetic towards DEP, DBP and DOP. Since the template of surface molecularly imprinted polymers could be changed with the analytes, MIPsbased molecularly imprinted polymers combining with hollow ber stirring bar sorptive extraction can be a promising and selective method for separation and extraction of series analytes with similar structure in complicated samples without sample clean-up.

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Dual-recognition membrane Adsorbers combining hydrophobic charge-induction chromatography with surface imprinting via multicomponent reaction

Cited by 9 publications

References 44 publications

Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Nanofibrous Membranes with High Mechanical Strength for the Separation and Purification of Lysozyme

Simultaneous Recovery of Bovine Serum Albumin and Bovine Immunoglobulin G with Dual-Ligand Hydrophobic Charge-Induction Chromatography

Dummy Template–Based Molecularly Imprinted Solid-Phase Microextraction Coating for Analysis of Plasticizers in Food Samples

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