Dual regulation of the Na+/H+‐exchange in rat peritoneal mast cells: role of protein kinase C and calcium on pHi regulation and histamine release

Friis, Ulla Glenert; Johansen, Torben

doi:10.1111/j.1476-5381.1996.tb15541.x

Cited by 14 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibition of Na + /K + -ATPase may induce an inhibition on the activity of the Na + /H + exchanger [13]. On the other hand, the activation of PKC in rat mast cells results in an increase in the Na + /H + -antiporter activity [3,12]. This same effect was also described in lymphocytes and platelets [14,15].…”

Section: Introductionsupporting

confidence: 53%

“…The activity of the Na + /H + antiporter is modulated by different phosphorylation pathways [3,7,10,12]. The phorbol esther PMA, a PKC activator, is known to increase the velocity of the Na + /H + transporter [12,39].…”

Section: Discussionmentioning

confidence: 99%

“…They secrete their granules through a fast exocytosis process. These cells are non-excitable, their low membrane potential being regulated by monovalent plasma membrane fluxes through several exchangers [1], among them the Na + /H + exchanger [2,3].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Sodium, PMA and Calcium Play an Important Role on Intracellular pH Modulation in Rat Mast Cells

Alfonso¹,

Botana²,

Vieytes³

et al. 1998

Cell Physiol Biochem

View full text Add to dashboard Cite

In a series of experiments aimed to understand the signaling pathways that regulate intracellular pH (pHi) in rat mast cells, the effect of different intracellular mechanisms on the activity of the Na⁺/H⁺ exchanger was studied. After promoting an artificial acidification with sodium propionate we determined the variations on pHi rate recovery. pHi was measured with the dye 2,7-bis(carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester. We studied the effect that the inhibition of some cellular exchangers with different drugs induced on pHi. When the Na⁺/H⁺ exchanger was inhibited in the presence of amiloride, the recovery rate constant was twofold smaller than the control value. After the recovery, the final pH was lower than the initial value when the cells were treated either with amiloride or with 4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid (an anionic antiport inhibitor). No effect was observed when the Na⁺/K⁺-ATPase or the Na⁺/Ca²⁺ exchanger were inhibited. The suppression of intracellular and extracellular calcium did not induced any change in pHi. The addition of thapsigargin, an activator of capacitative calcium influx, or the phorbol esther 12-O-tetradecanoylphorbol-13-acetate (PMA), a protein kinase C (PKC) activator, increased the activity of the antiporter. Both effects were abrogated by inhibition of the Na⁺/K⁺-ATPase with ouabain. The increase in cAMP levels did not affect the effect of PMA on pHi recovery, but it blocked the effect of thapsigargin. Our results indicate that rat mast cells regulate pHi by the combination of some anionic exchanger and the Na⁺/H⁺ antiporter. And also that the modulation of this exchanger is the consequence of the connection between different intracellular mechanisms, Na⁺/K⁺-ATPase-PKC-calcium, among which cAMP seems not to have a direct role.

show abstract

Section: Introductionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Sodium, PMA and Calcium Play an Important Role on Intracellular pH Modulation in Rat Mast Cells

Alfonso¹,

Botana²,

Vieytes³

et al. 1998

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Although the precise mode of action of compound 48/80 is unknown it has become evident that both the mobilization and the influx of Ca 2þ and the activation of PKC are required for manifest exocytosis, i.e., the release of histamine from the granules into the extracellular environment [4]. To which extent eicosanoids play a role in the degranulation process is not clear, but it cannot be excluded that these substances support the signal transduction process.…”

Section: Introductionmentioning

confidence: 98%

Dietary modulation of fatty acid composition of mast cell phospholipids does not affect histamine release induced by compound 48/80

Engels

Haaster

Lemmens

et al. 1997

Inflammation Research

View full text Add to dashboard Cite

show abstract

“…Thus far, only chloride channels [4,5] and two electrolyte transporters have been studied in mast cells, one is the Na + /K + -adenosine triphosphatase (ATPase) [6] and the other the Na + /H + exchanger [7][8][9]. The aspects of a possible relationship between calcium and sodium in the mast cell response have been studied by a few authors [10][11][12][13], and we have previously reported that in rat mast cells, there is an active Na + /H + exchanger [7] and no sodium channels [14].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Na+/Ca2+ Exchanger on Rat Mast Cells

Alfonso¹,

Lago²,

Botana

et al. 1999

Cell Physiol Biochem

View full text Add to dashboard Cite

The Na⁺/Ca²⁺ exchanger has not been characterized in rat mast cells, although its exitstence has been previously suggested. In this work, we determine that this exchanger exists on rat mast cells and that it has an important regulatory role on the cellular function. We have studied uptake and release of ⁴⁵Ca in the presence of different external sodium concentrations and, under the same conditions, the simultaneous uptake of ²²Na and ⁴⁵Ca. The results show that uptake and release of ⁴⁵Ca in these cells are related to the concentration of sodium in the extracellular medium and that there is also a perfect coupling between ²²Na and ⁴⁵Ca fluxes. In these conditions, we evaluated the intracellular calcium levels in fura-2 loaded cells. When the extracellular sodium concentration was lower than 60 mM, we observed an increase in intracellular calcium, reaching its maximum when the extracellular medium has no sodium. Then we investigated the effect on histamine release. The ionophore A23187 elicits histamine release in rat mast cells, depending on the extracellular calcium concentration. This drug releases more histamine (up to twofold) with sodium concentrations <60 mM. In the presence of 2,4-dichlorobenzamil hydrochloride, a Na⁺/Ca²⁺ exchanger inhibitor, the release of histamine induced by the ionophore was lower than in controls in media with low external sodium, and, on the contrary, at extracellular sodium concentrations >60 mM, the histamine release was higher than in controls. In the same conditions, but when the Na⁺-K⁺ ATPase was inhibited by ouabain, and as a consequence more sodium was inside the cells, a high increase in histamine release induced by A23187 in a sodium-free medium was observed. Under the same conditions, a high increase in intracellular calcium takes place. The overall data are preliminary evidence suggesting the existence of a Na⁺/Ca²⁺ exchanger in rat mast cells with a threshold to get reversed at 60 mM external sodium, lower concentrations of this ion increasing internal calcium and producing higher histamine release.

show abstract

Dual regulation of the Na⁺/H⁺‐exchange in rat peritoneal mast cells: role of protein kinase C and calcium on pH_i regulation and histamine release

Cited by 14 publications

References 29 publications

Sodium, PMA and Calcium Play an Important Role on Intracellular pH Modulation in Rat Mast Cells

Sodium, PMA and Calcium Play an Important Role on Intracellular pH Modulation in Rat Mast Cells

Dietary modulation of fatty acid composition of mast cell phospholipids does not affect histamine release induced by compound 48/80

Characterization of the Na<sup>+</sup>/Ca<sup>2+</sup> Exchanger on Rat Mast Cells

Contact Info

Product

Resources

About