Dual role far red fluorescent molecular rotor for decoding the plasma membrane and mitochondrial viscosity

Silswal, Akshay; Pramanik, Anup; Koner, Apurba Lal

doi:10.1039/d3tb02346j

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…As shown in Figure d–g, both of the merged channels showed an excellent overlap, further demonstrating the organelles (mitochondria and nucleolus) targeted by SYN before and after mitochondrial damage. In addition, due to the presence of a high-viscosity environment in the mitochondrial matrix and nucleolus, the rotor effect in SYN gives it good imaging quality for mitochondria and nucleolus. , After irradiation, the localization of SYN for other organelles (such as lysosome and endoplasmic reticulum) was also inspected using commercial dyes (Figure S9), the poor overlap of SYN and the commercial dyes indicated that SYN did not target other organelles. Additionally, images of live HeLa cells that were irradiated discontinuously and stained with SYN were obtained, as shown in Figure S10.…”

Section: Resultsmentioning

confidence: 99%

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Wang,

Lin,

Liu

et al. 2024

Anal. Chem.

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Visualization of the mitochondrial state is crucial for tracking cell life processes and diagnosing disease, while fluorescent probes that can accurately assess mitochondrial status are currently scarce. Herein, a fluorescent probe named "SYN" was designed and prepared, which can target mitochondria via the mitochondrial membrane potential. Upon pathology or external stimulation, SYN can be released from the mitochondria and accumulate in the nucleolus to monitor the status of mitochondria. During this process, the brightness of the nucleolus can then serve as an indicator of mitochondrial damage. SYN has demonstrated excellent photostability in live cells as well as an extremely inert fluorescence response to bioactive molecules and the physiological pH environment of live cells. Spectroscopic titration and molecular docking studies have revealed that SYN can be lit up in nucleoli due to the high viscosity of the nucleus and the strong electrostatic interaction with the phosphate backbone of RNA. This probe is expected to be an exceptional tool based on its excellent imaging properties for tracking mitochondrial state in live cells.

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Section: Resultsmentioning

confidence: 99%

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Wang,

Lin,

Liu

et al. 2024

Anal. Chem.

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“…In another variation, we modified the rotor by introducing a trimethyl ammonium cationic group, guiding the resulting JMT rotor to the mitochondria of cells. 38 JMT was then utilized to assess mitochondrial stress, leveraging the fact that under stress conditions, the viscosity of mitochondria tends to increase. Researchers have documented a distinct form of cell death called ferroptosis, which is dependent on iron and differs in appearance and biochemical characteristics from apoptosis, necrosis, and autophagy.…”

Section: Applications Of Julolidine-based Fmrsmentioning

confidence: 99%

Julolidine-based fluorescent molecular rotor: a versatile tool for sensing and diagnosis

Chakraborty,

Silswal,

Koner

2024

Sens. Diagn.

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Morpholine Anchored Fluorogenic Toolkit: Unveiled Disease Allied Protein Fibrillation in Lysosomal Compartment of Live‐Cell and Drosophila Models

P.,

Wilson,

Silswal

et al. 2024

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The aberrant accumulation of cytotoxic protein aggregates is a hallmark of various neurodegenerative and non‐neurodegenerative ailments, necessitating the development of sensitive and selective tools for their detection. Herein, we report a series of morpholine‐anchored fluorescent probes, denoted as SC‐nmor (n = 2, 4, 6), designed for facile visualization of protein aggregates. These probes display notable changes in their photophysical properties upon binding with protein aggregates, owing to their high sensitivity to the fibrillar microenvironment. Specifically, the SC‐4mor probe demonstrates strong selectivity for aggregated insulin proteins over native insulin, accompanied by a significant enhancement in fluorescence lifetime. Live‐cell imaging reveals an exclusive localization at the lysosomal compartment. This feature enables the visualization of lysosomal accumulated protein fibrils induced with pepstatin A. Additionally, in vivo assessments on genetically mutated and dietary‐modified Drosophila melanogaster, representing neurodegenerative and non‐neurodegenerative disease models, demonstrate staining of protein aggregates. The enhanced emission from the eye lobes of Aβ‐mutated and HSD brain samples, suggesting that SC‐4mor can exhibit adequate retention in the brain with minimal biological toxicity. SC‐4mor also shows its capability to cross the blood–brain barrier in mice model. Consequently, SC‐4mor emerges as a promising marker for detecting and monitoring neurotoxic protein fibrillation in live cells and animal models, offering potential insights into the pathogenesis and progression of protein aggregation.

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Dual role far red fluorescent molecular rotor for decoding the plasma membrane and mitochondrial viscosity

Abstract: Rationally designed Julolidine-based molecular rotor (JMT) targets mitochondria with far-red emission accounting for mitochondrial dysfunction. Further, the communication between mitochondria and plasma membrane was investigated upon mitochondrial depolarization.

Cited by 3 publications

References 54 publications

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Lighting Up Nucleolus To Report Mitochondria Damage Using a Mitochondria-to-Nucleolus Migration Probe

Julolidine-based fluorescent molecular rotor: a versatile tool for sensing and diagnosis

Morpholine Anchored Fluorogenic Toolkit: Unveiled Disease Allied Protein Fibrillation in Lysosomal Compartment of Live‐Cell and Drosophila Models

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