Dual role of myosin II during Drosophila imaginal disc metamorphosis

Aldaz, Silvia; Escudero, Luis; Freeman, Matthew

doi:10.1038/ncomms2763

Cited by 47 publications

(49 citation statements)

References 41 publications

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“…Previous research has shown apically localized Sqh to be involved in apical constriction, invagination and MyoII-dependent regulation of tension in epithelia (Aldaz et al, 2013; Monier et al, 2015; Zimmerman et al, 2010). We therefore examined whether knocking down sqh via RNAi may reduce cell survival or promote basal extrusion.…”

Section: Resultsmentioning

confidence: 98%

“…6H) and miR-8 mutants also exhibit increased Ena (Loya et al, 2009). These proteins are involved in cortical F-actin bundling, F-actin polymerization and nucleation at adherens junctions and actomyosin contraction - molecular functions consistent with alterations in cell migration, cell-cell junction remodeling and cytokinesis, which may contribute to the G2 arrest of miR-8 expressing cells (Aldaz et al, 2013; Founounou et al, 2013; Geisbrecht and Montell, 2002; Koch et al, 2012; Lin et al, 2007; Mavrakis et al, 2014). …”

Section: Resultsmentioning

confidence: 99%

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miR-8 modulates cytoskeletal regulators to influence cell survival and epithelial organization in Drosophila wings

Bolin

Rachmaninoff

Moncada

et al. 2016

Developmental Biology

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Summary The miR-200 microRNA family plays important tumor suppressive roles. The sole Drosophila miR-200 ortholog, miR-8 plays conserved roles in Wingless, Notch and Insulin signaling pathways linked to tumorigenesis, yet homozygous null animals are viable and often appear morphologically normal. We observed that wing tissues mosaic for miR-8 levels by genetic loss or gain of function exhibited patterns of cell death consistent with a role for miR-8 in modulating cell survival in vivo. Here we show that miR-8 levels impact several actin cytoskeletal regulators that can affect cell survival and epithelial organization. We show that loss of miR-8 can confer resistance to apoptosis independent of an epithelial to mesenchymal transition while the persistence of cells expressing high levels of miR-8 in the wing epithelium leads to increased JNK signaling, aberrant expression of extracellular matrix remodeling proteins and disruption of proper wing epithelial organization. Altogether our results suggest that very low as well as very high levels of miR-8 can contribute to hallmarks associated with cancer, suggesting approaches to increase miR-200 microRNAs in cancer treatment should be moderate.

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Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

miR-8 modulates cytoskeletal regulators to influence cell survival and epithelial organization in Drosophila wings

Bolin

Rachmaninoff

Moncada

et al. 2016

Developmental Biology

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“…Finally, the RLC-AE mutant had the most severe defect in the ATP hydrolysis rate and correspondingly had the most significant defect in constriction rate and tissue folding. Past work has suggested that RLC-EE can promote contraction (Aldaz et al, 2013; Escudero et al, 2007; Kasza et al, 2014). However, this result is only obtained when the RLC-EE is expressed in a background where wild-type RLC-TS is also present.…”

Section: Discussionmentioning

confidence: 99%

Drosophila non-muscle myosin II motor activity determines the rate of tissue folding

Vasquez

Heissler

Billington

et al. 2016

eLife

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Non-muscle cell contractility is critical for tissues to adopt shape changes. Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. Here, combining the biochemical analysis of enzymatic and motile properties for purified myosin mutants with in vivo measurements of apical constriction for the same mutants, we show that in vivo constriction rate scales with myosin motor activity. We show that so-called phosphomimetic mutants of the Drosophila regulatory light chain (RLC) do not mimic the phosphorylated RLC state in vitro. The defect in the myosin motor activity in these mutants is evident in developing Drosophila embryos where tissue recoil following laser ablation is decreased compared to wild-type tissue. Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos.DOI: http://dx.doi.org/10.7554/eLife.20828.001

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“…Regulation of cell size and shape is known to be driven by integrins under the control of the Ras‐signaling cascade for changes in cell shape (Domínguez‐Giménez et al, ), whereas patterns of oriented cell intercalation could be regulated by the Ft‐Ds‐Fj system as it is the case in the thorax (Bosveld et al, ) or by the Zipper, Sqh and RhoA proteins, as is the case in the embryo (Bertet et al, ). Intercalation could also be driven by external forces coming from myosin II activity in the peripodal membrane or from the hydraulic pressure of pumped hemolymph (Taylor & Adler, ; Aldaz et al, ). Note that the peripodial epithelium is also necessary to perforate the larval epidermis and it presumably contracts to allow the exit of the notum and of the wing blade through the larval epidermis (Fristrom & Fristrom, ; Pastor‐Pareja et al, ) (Fig.…”

Section: Morphological Variation Generated During Developmentmentioning

confidence: 99%

Making quantitative morphological variation from basic developmental processes: Where are we? The case of the Drosophila wing

Matamoro‐Vidal

Salazar‐Ciudad

Houle

2015

Developmental Dynamics

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One of the aims of evolutionary developmental biology is to discover the developmental origins of morphological variation. The discipline has mainly focused on qualitative morphological differences (e.g., presence or absence of a structure) between species. Studies addressing subtle, quantitative variation are less common. The Drosophila wing is a model for the study of development and evolution, making it suitable to investigate the developmental mechanisms underlying the subtle quantitative morphological variation observed in nature. Previous reviews have focused on the processes involved in wing differentiation, patterning and growth. Here, we investigate what is known about how the wing achieves its final shape, and what variation in development is capable of generating the variation in wing shape observed in nature. Three major developmental stages need to be considered: larval development, pupariation, and pupal development. The major cellular processes involved in the determination of tissue size and shape are cell proliferation, cell death, oriented cell division and oriented cell intercalation. We review how variation in temporal and spatial distribution of growth and transcription factors affects these cellular mechanisms, which in turn affects wing shape. We then discuss which aspects of the wing morphological variation are predictable on the basis of these mechanisms.

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Dual role of myosin II during Drosophila imaginal disc metamorphosis

Cited by 47 publications

References 41 publications

miR-8 modulates cytoskeletal regulators to influence cell survival and epithelial organization in Drosophila wings

miR-8 modulates cytoskeletal regulators to influence cell survival and epithelial organization in Drosophila wings

Drosophila non-muscle myosin II motor activity determines the rate of tissue folding

Making quantitative morphological variation from basic developmental processes: Where are we? The case of the Drosophila wing

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