Dual roles of CYP302A1 in regulating ovarian maturation and molting in Macrobrachium nipponense

Zheng, Yalu; Zhang, Wenyi; Xiong, Yiwei; Wang, Jisheng; Jin, Shubo; Qiao, Hui; Jiang, Sufei; Fu, Hongtuo

doi:10.1016/j.jsbmb.2023.106336

Cited by 5 publications

(1 citation statement)

References 51 publications

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“…Our long-term interference experiment results showed that Mn-NPC1 knockdown significantly reduced the GSI and ecdysone contents of female M. nipponense. In contrast with our previous study on the Halloween family genes (such as CYP307 A1, CYP302 A1, and CYP306A1), knockdown of Mn-NPC1 had a lesser effect on ovarian maturation delay and decrease of molting frequency than knockdown of the Halloween family genes [34][35][36]. These results suggest that while NPC1 is an important regulator of sterol transport in M. nipponense, it is not the only factor involved.…”

Section: Discussioncontrasting

confidence: 99%

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

Jiang,

Zhang,

Xiong

et al. 2024

IJMS

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NPC intracellular cholesterol transporter 1 (NPC1) plays an important role in sterol metabolism and transport processes and has been studied in many vertebrates and some insects, but rarely in crustaceans. In this study, we characterized NPC1 from Macrobrachium nipponense (Mn-NPC1) and evaluated its functions. Its total cDNA length was 4283 bp, encoding for 1344 amino acids. It contained three conserved domains typical of the NPC family (NPC1_N, SSD, and PTC). In contrast to its role in insects, Mn-NPC1 was mainly expressed in the adult female hepatopancreas, with moderate expression in the ovary and heart. No expression was found in the embryo (stages CS–ZS) and only weak expression in the larval stages from hatching to the post-larval stage (L1–PL15). Mn-NPC1 expression was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. A 25-day RNA interference experiment was employed to illustrate the Mn-NPC1 function in ovary maturation. Experimental knockdown of Mn-NPC1 using dsRNA resulted in a marked reduction in the gonadosomatic index and ecdysone content of M. nipponense females. The experimental group showed a significant delay in ovarian maturation and a reduction in the frequency of molting. These results expand our understanding of NPC1 in crustaceans and of the regulatory mechanism of ovarian maturation in M. nipponense.

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Section: Discussioncontrasting

confidence: 99%

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

Jiang,

Zhang,

Xiong

et al. 2024

IJMS

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The Dmrt1 gene: Identification and functional analysis in testicular development and spermatogenesis in the freshwater prawn, Macrobrachium nipponense

Wan,

Yu,

Zhang

et al. 2025

Aquaculture

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CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles

Chen,

Li,

Fang

et al. 2024

Sci Rep

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The bean beetle ( Callosobruchus maculatus ) clearly exhibits population density-dependent polymorphism. Cytochrome P450 (CYP) is involved in many aspects of the physiological activities of insects. However, the role of CYP in population density-dependent polymorphisms remains unknown. The terminal oocyte maturity rate of high-population-density individuals (H) was faster than that of low-population-density individuals (L). A total of 56 CYP-like genes were identified from transcriptomic and genomic data, including seven clan 2 CYP-like genes, seven mitochondrial CYP-like genes, 19 clan 3 CYP-like genes, and 23 clan 4 CYP-like genes. Gene duplication might occur in CYP9Z4-like , CYP345A1-like , CYP345A2-like , CYP349A1-like , CYP349A2-like , and CYP4BN4-like . Thirteen and two CYP-like genes were up-regulated and down-regulated, respectively, in H. Among these CYP-like genes, CYP4BN4v7-like was the most abundant CYP. CYP4BN4v7-like was more highly expressed in the head than in the thorax and abdomen. Its mRNA levels in the head, thorax, and abdomen were greater in H than in L. After RNA interference decreased its mRNA level, the terminal oocyte maturity rate decreased. Moreover, the expression level of insulin-like peptide 1 ( ILP1 ), which plays a vital role in regulating terminal oocyte development, decreased in the head. In conclusion, CYP4BN4v7 - like modulated the population density-dependent terminal oocyte maturity rate by regulating the expression of ILP1 . Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-79866-2.

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Dual roles of CYP302A1 in regulating ovarian maturation and molting in Macrobrachium nipponense

Cited by 5 publications

References 51 publications

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

The Dmrt1 gene: Identification and functional analysis in testicular development and spermatogenesis in the freshwater prawn, Macrobrachium nipponense

CYP4BN4v7 regulates the population density dependent oocyte maturity rate in bean beetles

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