Dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase

Cai, Fudong; Wang, Nan; Dong, Ting; Deng, Anping; Li, Jianguo

doi:10.1039/c5an00999e

Cited by 20 publications

(10 citation statements)

References 28 publications

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“…This method is high sensitivity and easy to use and has been used for the detection of pork and liver samples. The similar method was also reported by Cai et al…”

Section: Qd Application In Detection Of Pesticide and Veterinary Drug...supporting

confidence: 66%

Quantum Dots Applied to Methodology on Detection of Pesticide and Veterinary Drug Residues

Zhou

Zou

Song

et al. 2018

J. Agric. Food Chem.

116

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The pesticide and veterinary drug residues brought by large-scale agricultural production have become one of the issues in the fields of food safety and environmental ecological security. It is necessary to develop the rapid, sensitive, qualitative and quantitative methodology for the detection of pesticide and veterinary drug residues. As one of the achievements of nanoscience, quantum dots (QDs) have been widely used in the detection of pesticide and veterinary drug residues. In these methodology studies, the used QD-signal styles include fluorescence, chemiluminescence, electrochemical luminescence, photoelectrochemistry, etc. QDs can also be assembled into sensors with different materials, such as QD-enzyme, QD-antibody, QD-aptamer, and QD-molecularly imprinted polymer sensors, etc. Plenty of study achievements in the field of detection of pesticide and veterinary drug residues have been obtained from the different combinations among these signals and sensors. They are summarized in this paper to provide a reference for the QD application in the detection of pesticide and veterinary drug residues.

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“…This method is high sensitivity and easy to use and has been used for the detection of pork and liver samples. The similar method was also reported by Cai et al…”

Section: Qd Application In Detection Of Pesticide and Veterinary Drug...supporting

confidence: 66%

Quantum Dots Applied to Methodology on Detection of Pesticide and Veterinary Drug Residues

Zhou

Zou

Song

et al. 2018

J. Agric. Food Chem.

116

View full text Add to dashboard Cite

show abstract

“…16,17 Routine approaches consist of ligandconjugated enzyme labels and metal-containing nanolabels. 18,19 In contrast with nanolabels, unfavorably, enzymebased labeling strategies are susceptible to interference and assay conditions during the signal-generation stage, e.g., pH, temperature and the instability caused by structural unfolding. 20 Inspiringly, the emergence of nanocatalysts opens a new horizon for the development of advanced nanolabels for signal amplification.…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Sun

2015

Analyst

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A new electrochemical immunosensor with signal enhancement was designed for sensitive detection of disease-related protein (human carbohydrate antigen 19-9, CA 19-9 used in this case). The assay was carried out on a capture antibody-modified screen-printed carbon electrode with a sandwich-type mode by using detection antibody-functionalized nanogold-encapsulated poly(amidoamine) dendrimer (AuNP-PAAD). The AuNP-PAAD was first synthesized through the in situ reduction method and functionalized with the polyclonal rabbit anti-human CA 19-9 antibody. Upon target CA 19-9 introduction, a sandwiched immunocomplex could be formed between the capture antibody and detection antibody. Accompanying the AuNP-PAAD, the electrocatalytic activity of the carried gold nanoparticles toward the hydrogen evolution reaction (HER) allowed the rapid quantification of the target analyte on the electrode. The amplified electrochemical signal mainly derived from AuNP-catalyzed HER in an acidic medium. Under optimal conditions, the immuno-HER assay displayed a wide dynamic concentration range from 0.01 to 300 U mL(-1) toward target CA 19-9 with a detection limit (LOD) of 6.3 mU mL(-1). The reproducibility, precision, specificity and stability of our strategy were acceptable. Additionally, the system was further validated by assaying 13 human serum specimens, giving well matched results obtained from the commercialized enzyme-linked immunosorbent assay (ELISA) method.

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“…AuNPs provided a matrix to anchor a large amount of HRP which greatly enhanced the electrochemical quenching. LODs for salbutamol and ractopamine were 17 pg/mL and 1.7 pg/mL, respectively [92,93].…”

Section: Electrochemiluminescence Immunosensormentioning

confidence: 98%

Current Advances in Immunoassays for the Detection of β2-Agonists

Ouyang

2022

Foods

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β2-agonists are a group of synthetic phenylethanolamine compounds which are traditionally used for treating bronchospasm. These compounds can also increase skeletal muscle mass and decrease body fat. The illegal use of β2-agonists in food-producing animals results in residue of β2-agonists in edible tissues and causes adverse health effects in humans. Thus, the detection of β2-agonists at trace level in complex sample matrices is of great importance for monitoring the abuse of β2-agonists. Many methods have been developed to detect β2-agonists. Among them, a variety of antigen–antibody interaction-based techniques have been established to detect β2-agonists in various samples, including animal feed, urine, serum, milk, tissues and hair. In this review, we summarized current achievement in the extraction of β2-agonists from testing samples and detection of β2-agonists using immunological techniques. Future perspectives were briefly discussed.

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Dual-signal amplified electrochemiluminescence immunoassay for salbutamol based on quantum dots and gold nanoparticle-labeled horseradish peroxidase

Cited by 20 publications

References 28 publications

Quantum Dots Applied to Methodology on Detection of Pesticide and Veterinary Drug Residues

Quantum Dots Applied to Methodology on Detection of Pesticide and Veterinary Drug Residues

Sensitive electrochemical immunoassay with signal enhancement based on nanogold-encapsulated poly(amidoamine) dendrimer-stimulated hydrogen evolution reaction

Current Advances in Immunoassays for the Detection of β2-Agonists

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