Dual-signal output paper sensor based on coordinative self-assembly biomimetic nanozyme for point-of-care detection of biomarker

Du, Yuanchun; Ke, Zhenyi; Zhang, Jiaheng; Feng, Guangfu

doi:10.1016/j.bios.2022.114656

Cited by 14 publications

(5 citation statements)

References 52 publications

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“…Abnormal levels of cholesterol can lead to hyperlipidemia, atherosclerosis, anemia, and other diseases . Accordingly, the development of simple and reliable assay methods is essential. , Considering that cholesterol is catalyzed by cholesterol oxidase to generate H 2 O 2 , a visual, label-free, highly sensitive, and selective biosensing strategy was established for the facile detection of cholesterol on the basis of POD-mimicking CuNAD (Figure a). With the increase in cholesterol concentration, the solutions gradually change from colorless to deep blue (Figure S21).…”

Section: Resultsmentioning

confidence: 99%

Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Zhang

Tan

et al. 2023

Anal. Chem.

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Nanozymes can imitate the catalytic properties of natural enzymes while overcoming the limitations of natural enzymes such as high cost, poor robustness, and difficulty in recycling. However, rational design and facile preparation of nanozymes are still in demand. Inspired by the chemical structure of laccase, we report an amorphous metal–organic coordination nanocomposite named CuNAD, which is composed of copper ions and nicotinamide adenine dinucleotide (NAD+) via a simple coordinating coassembly process. As a single-site nanozyme, CuNAD exhibits excellent robustness under extreme conditions, significantly stronger catalytic activity for phenolic compounds, and 4.02-fold higher sensitivity for epinephrine detection than laccase. Furthermore, by breaking through the functional constraints of laccase, CuNAD is also able to activate H2O2 at neutral pH, benefiting a one-step chromogenic detection platform for cholesterol. This facile approach demonstrates the potential to develop single-site nanozymes by biomimicking natural enzymes and may boost more insights into the structure–function relationship of nanozymes.

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Section: Resultsmentioning

confidence: 99%

Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Zhang

Tan

et al. 2023

Anal. Chem.

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“…41,42 NP accumulation reactions are also applied in point-of-care devices including lateral flow assay (LFA) and paper-based colorimetric biosensing systems. [43][44][45][46][47] In order to overcome the disadvantage of the narrow analytical spectrum of colorimetric biosensors, it is an excellent extension to introduce the application of fluorescence biosensing systems. Unlike colorimetric biosensors, fluorescence biosensors reflect changes in the concentration of the analyte mainly by differences in the intensity of fluorescence-containing optical-sensing molecules, with the advantages of high sensitivity and fast real-time monitoring.…”

Section: Colorimetric and Fluorescence Biosensingmentioning

confidence: 99%

Optical biosensing systems for a biological living body

Yan

Guo

Wang

et al. 2023

VIEW

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With the development of technology, biosensors are increasingly used in the biomedical field. Due to the high sensitivity of optical signals, good stability, high signal‐to‐noise ratio, and different spectral characteristics of different analytes, optical biosensors can achieve direct and real‐time detection of analytes with high specificity compared with traditional analytical techniques. In view of the rapid development of optical biosensors for in vivo applications and their promising future, we have attempted to summarize the working principles and recent advances in application in humans, with the aim of helping readers to gain an in‐depth and detailed understanding of optical biosensors developed in recent years for applications in the biological living body. In this review, we focus on some of the current widely used and promising optical biosensors, including colorimetric biosensors, fluorescence biosensors, and surface‐enhanced‐Raman‐scattering‐based biosensors. The working principles for each type of optical biosensor are concisely described and the application of the biosensor in the living body is summarized by category. We conclude by looking at the prospects of in vivo applications of optical biosensors.

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“…Sensing methods incorporating various nanozymemediated signal amplification techniques have diversified into test paper sensing, electrochemical sensing, colorimetric sensing, and SERS sensing. These methods are utilized for the detection of ions (e.g., heavy metal ions Hg 2+ [31] and nonmetal ions CN − [32] ), small molecules (cholesterol, [33] uric acid (UA), [34] glutathione/GSH, [35] and glucose [36] ), nucleic acids, [37] proteins, [38] cancer cells, [39] and virus. [40] A summary of these sensing modes is provided in Tables (Tables 1-4).…”

Section: Introductionmentioning

confidence: 99%

“…[47] This review aims to address this gap and provide a comprehensive explanation of the strategy for optimizing the sensitivity of the assay. Invertase/antibody-AuNPs POD 8-hydroxy-2-deoxyguanosine (0.23 ng mL −1 ) PSA (1.26 ng mL −1 ) [49] Au@Ag-Pt NPs POD CRP (15 pg mL −1 ) [50] Au@Pt OXD HBV-DNA (8.5 pm) [51] Other Nanozymes with multi-enzyme activity enhance T-line signal readout through cascading catalytic reactions 3,3′,5,5′-tetra-methylbenzidine (TMB)-hemin-bovine serum albumin (BSA)-acetylcholinesterase-chromogen POD Paraoxon (0.115 ng mL −1 ) [62] Nanozymes enable diverse signal enhancement readout methods Fe-GMP-L-His coordination polymer NPs POD Cholesterol (0.05 μm) [33] Cu-anchored polydopamine (PDA) POD Aspergillus flavus (0.22 ng mL −1 ) [63] Hybridized nanozymes with enhanced enzymatic activity as signal probes for signal amplification…”

Section: Introductionmentioning

confidence: 99%

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

Wang,

Liu,

Fan

2023

Small Methods

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Nanozymes show great promise in enhancing disease biomarker sensing by leveraging their physicochemical properties and enzymatic activities. These qualities facilitate signal amplification and matrix effects reduction, thus boosting biomarker sensing performance. In this review, recent studies from the last five years, concentrating on disease biomarker detection improvement through nanozyme‐based biosensing are examined. This enhancement primarily involves the modulations of the size, morphology, doping, modification, electromagnetic mechanisms, electron conduction efficiency, and surface plasmon resonance effects of nanozymes for increased sensitivity. In addition, a comprehensive description of the synthesis and tuning strategies employed for nanozymes has been provided. This includes a detailed elucidation of their catalytic mechanisms in alignment with the fundamental principles of enhanced sensing technology, accompanied by the presentation of quantitatively analyzed results. Moreover, the diverse applications of nanozymes in strip sensing, colorimetric sensing, electrochemical sensing, and surface‐enhanced Raman scattering have been outlined. Additionally, the limitations, challenges, and corresponding recommendations concerning the application of nanozymes in biosensing have been summarized. Furthermore, insights have been offered into the future development and outlook of nanozymes for biosensing. This review aims to serve not only as a reference for enhancing the sensitivity of nanozyme‐based biosensors but also as a catalyst for exploring nanozyme properties and their broader applications in biosensing.

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Dual-signal output paper sensor based on coordinative self-assembly biomimetic nanozyme for point-of-care detection of biomarker

Cited by 14 publications

References 52 publications

Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Bioinspired Coassembly of Copper Ions and Nicotinamide Adenine Dinucleotides for Single-Site Nanozyme with Dual Catalytic Functions

Optical biosensing systems for a biological living body

Signal Amplification Strategy Design in Nanozyme‐Based Biosensors for Highly Sensitive Detection of Trace Biomarkers

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