Dual Skin-Whitening and Anti-wrinkle Function of Low-Molecular-Weight Fish Collagen

Fish Collagen Peptide (NaticolⓇ) Protects the Skin from Dryness, Wrinkle Formation, and Melanogenesis Both In Vitro and In Vivo

“…Hs27 (human fibroblasts) and B16F10 (melanoma) cells were purchased from the American Type Culture Collection (Manassas, VA, USA). Each cell line was cultured, and cells were exposed to UVB or treated with 3-isobutyl-1-methylxanthine (IBMX) according to the methods described previously ( Park et al, 2021 ; Kim et al, 2022 ). Each cell line was treated with ascorbic acid (100 µg/mL), arbutin (100 µg/mL), and Naticol Ⓡ (100, 200, or 400 µg/mL).…”

“…HaCaT cells and B16F10 cells were lysed, and the levels HA, sphingomyelin, pro-inflammatory cytokines, melanin, GSH, tyrosinase, NO, and cAMP were measured according to the methods described previously ( Park et al, 2021 ; Kim et al, 2022 ).…”

“…Mice were allocated into six groups (n=8 per group): normal control (NC; no UVB irradiation), control (C; irradiated with UVB), positive control 1 (PC 1; irradiated with UVB and administered L ascorbic acid at 200 mg/kg), positive control 2 (PC 2; irradiated with UVB and administered arbutin at 200 mg/kg), Naticol Ⓡ 150 (irradiated with UVB and administered Naticol Ⓡ 150 mg/kg), Naticol Ⓡ 300 (irradiated with UVB and administered Naticol Ⓡ 300 mg/kg). The UVB dose schedule was described previously ( Park et al, 2021 ; Kim et al, 2022 ). The experiments were approved by the Institutional Animal Care and Use Committee of Kyung Hee University (protocol no.…”

“…The hydration, wrinkle formation, and thickness of the dorsal skin were measured according to methods described previously ( Park et al, 2021 ; Kim et al, 2022 ).…”

“…Increasing scientific evidence has shown that collagen hydrolysate from fish skin gelatin prevents wrinkle formation, melanogenesis, and skin dryness. We reported previously that collagen or collagen hydrolysate supplementation contributes to good skin health ( Kang et al, 2018 ; Lee et al, 2019 ; Kang et al, 2021 ; Park et al, 2021 ; Kim et al, 2022 ). Previous studies have reported that fish collagen peptide could weaken intestinal inflammation and treat diet-induced obesity and associated disorders ( Astre et al, 2018 ; Rahabi et al, 2022 ).…”