Dual-slit confocal light sheet microscopy for in vivo whole-brain imaging of zebrafish

Yang, Zhe; Mei, Li; Xia, Fei; Luo, Qingming; Fu, Ling; Gong, Hui

doi:10.1364/boe.6.001797

Cited by 31 publications

(14 citation statements)

References 39 publications

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“…Figure 25 Some of the sCMOS cameras are built in two separate detectors. Such architecture allows the parallelization of the acquisition in two separated arms, doubling the acquisition speed of the system [173].…”

Section: 2a Confocal Line Scanningmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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This paper is intended to give a comprehensive review of light-sheet (LS) microscopy from an optics perspective. As such, emphasis is placed on the advantages that LS microscope configurations present, given the degree of freedom gained by uncoupling the excitation and detection arms. The new imaging properties are first highlighted in terms of optical parameters and how these have enabled several biomedical applications. Then, the basics are presented for understanding how a LS microscope works. This is followed by a presentation of a tutorial for LS microscope designs, each working at different resolutions and for different applications. Then, based on a numerical Fourier analysis and given the multiple possibilities for generating the LS in the microscope (using Gaussian, Bessel, and Airy beams in the linear and nonlinear regimes), a systematic comparison of their optical performance is presented. Finally, based on advances in optics and photonics, the novel optical implementations possible in a LS microscope are highlighted.

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Section: 2a Confocal Line Scanningmentioning

confidence: 99%

Light-sheet microscopy: a tutorial

et al. 2018

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“…91 Dual-slit confocal light sheet microscopy was employed to image zebra fish brains at single-neuron resolution and proved to be twice as fast in imaging speed as a conventional line confocal microscope. 92 The high frame rate and the micron-scale spatial resolution are important advancements in neurobiology that enable the capture of neural activity and correlation in neuronal networks in whole zebrafish brain.…”

Section: Brain Imagingmentioning

confidence: 99%

Recent Progress in Light Sheet Microscopy for Biological Applications

Chatterjee

Pratiwi

et al. 2018

Appl Spectrosc

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The introduction of light sheet fluorescence microscopy (LSFM) has overcome the challenges in conventional optical microscopy. Among the recent breakthroughs in fluorescence microscopy, LSFM had been proven to provide a high three-dimensional spatial resolution, high signal-to-noise ratio, fast imaging acquisition rate, and minuscule levels of phototoxic and photodamage effects. The aforementioned auspicious properties are crucial in the biomedical and clinical research fields, covering a broad range of applications: from the super-resolution imaging of intracellular dynamics in a single cell to the high spatiotemporal resolution imaging of developmental dynamics in an entirely large organism. In this review, we provided a systematic outline of the historical development of LSFM, detailed discussion on the variants and improvements of LSFM, and delineation on the most recent technological advancements of LSFM and its potential applications in single molecule/particle detection, single-molecule super-resolution imaging, imaging intracellular dynamics of a single cell, multicellular imaging: cell-cell and cell-matrix interactions, plant developmental biology, and brain imaging and developmental biology.

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“…例如, 北京大学陈良怡小组近期发明并研制了基于超声可调制梯度透镜来扫描产生光片的大视场、高时空分辨新型双光子光片显微镜 [62] . 华中科技大学付玲小组设计了一种双光束光片照明显微扫描成像方法, 提升了光片显微镜的成像速率 [63] .…”

Section: 跨层次多参数生物光学成像及信息整合unclassified