Dual-specificity protein phosphatase Msg5 controls cell wall integrity and virulence in Fusarium oxysporum

Fernandes, Tania; Salvador, Encarnación Sánchez; Tapia, Ángela G.; Pietro, Antonio Di

doi:10.1016/j.fgb.2020.103486

Cited by 7 publications

(10 citation statements)

References 31 publications

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“…56,57 FO mutants lacking phosphatase Msg5 displayed reduced mycelial growth and were impaired in hyphal chemotropism toward host plant roots and in virulence on tomato plants. 58 Pheromone receptors could activate the chemotropic response of FO to the host plant, thus triggering the fungal infection. 59 Genes of pheromones α factor receptor (FOXG_10633), tyrosine-protein phosphatase MSG5 (FOXG_01836), and cell division control protein CDC24 (FOXG_04603 and FOXG_04602) in the MAPK signaling pathway of TR treatment in this study were all downregulated, indicating that Z-14 affects the virulence of FOC by affecting the secretion of cell wall-degrading enzymes and cell signaling.…”

Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Bacillus subtilis Strain Z-14 against Fusarium oxysporum Causing Vascular Wilt Diseases in Cucumber

Liu

Fan

Xiao

et al. 2023

J. Agric. Food Chem.

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Controlling cucumber Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC) with Bacillus strains is a hot research topic. However, the molecular mechanism of Bacillus underlying the biocontrol of cucumber wilt is rarely reported. In this study, B. subtilis strain Z-14 showed significant antagonistic activity against FOC, and the control effect reached 88.46% via pot experiment. Microscopic observations showed that strain Z-14 induced the expansion and breakage of FOC hyphae. The cell wall thickness was uneven, and the organelle structure was degraded. The combined analysis of metabolome and transcriptome showed that strain Z-14 inhibited the FOC infection by inhibiting the synthesis of cell wall and cell membrane, energy metabolism, and amino acid synthesis of FOC mycelium, inhibiting the clearance of reactive oxygen species (ROS) and the secretion of cell wall-degrading enzymes (CWDEs), thereby affecting mitogen-activated protein kinase (MAPK) signal transduction and inhibiting the transport function.

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Section: Journal Of Agricultural and Food Chemistrymentioning

confidence: 99%

Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Bacillus subtilis Strain Z-14 against Fusarium oxysporum Causing Vascular Wilt Diseases in Cucumber

Liu

Fan

Xiao

et al. 2023

J. Agric. Food Chem.

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“…The fact that inactivation of Tsc2 attenuates virulence of F. oxysporum on both plants and animals suggests that TORC1regulated mechanisms are relevant for infection in both types of hosts. Previous studies established that both the invasive growth MAPK Fmk1 and the CWI MAPK Mpk1 control critical steps during the infection process of F. oxysporum [5,8,53,55,62]. Collectively, these findings suggest that the virulence phenotypes observed upon TORC1 deregulation might be, at least in part, associated with defects in MAPK signaling.…”

Section: Torc1 Inhibits Fungal Virulence Functionsmentioning

confidence: 87%

“…Both Sit4 and another phosphatase called Ppg1 interact with protein phosphatase Msg5, a negative regulator of the CWI MAPK pathway [21]. Importantly, Msg5 was recently shown to regulate cell wall integrity and virulence in F. oxysporum [53]. Somewhat unexpectedly, expression of a gtr1 GTP allele, which was previously shown to activate TORC1 in different organisms [17, 30], was ineffective in F. oxysporum since no constitutive TORC1 activation was detected under the experimental conditions used in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The fungal cell wall provides a rigid cellular border and represents the first line of defense against adverse environmental conditions. In F. oxysporum, the cell wall is mainly composed of glucans, chitin and glycoproteins [50] and its integrity is essential for development and virulence [51][52][53]. Interestingly, S. cerevisiae cells expressing a hyperactive TOR1 allele were impaired in β-1-3-glucan synthesis and exhibited increased sensitivity to cell wall perturbing compounds [54].…”

Section: Fusarium Oxysporummentioning

confidence: 99%

Section: Torc1 Inhibits Fungal Virulence Functionsmentioning

confidence: 99%

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Constitutive activation of TORC1 signaling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum

Navarro-Velasco

Pietro

López‐Berges

2022

Preprint

Self Cite

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The filamentous fungus Fusarium oxysporum causes vascular wilt disease in a wide range of plant species and opportunistic infections in humans. Previous work suggested that invasive growth in this pathogen is controlled by environmental cues such as pH and nutrient status. Here we investigated the role of Target Of Rapamycin Complex 1 (TORC1), a global regulator of eukaryotic cell growth and development. Inactivation of the negative regulator Tuberous Sclerosis Complex 2 (Tsc2), but not constitutive activation of the positive regulator Gtr1, in F. oxysporum resulted in inappropriate activation of TORC1 signaling under nutrient limiting conditions. The tsc2 Δ mutants showed reduced colony growth on minimal medium with different nitrogen sources and increased sensitivity to cell wall or high temperature stress. Furthermore, these mutants were impaired in invasive hyphal growth across cellophane membranes and exhibited a marked decrease in virulence, both on tomato plants and on the invertebrate animal host Galleria mellonella. Importantly, invasive hyphal growth in tsc2 Δ strains was rescued by rapamycin-mediated inhibition of TORC1. Collectively, these results reveal a key role of TORC1 signaling in development and pathogenicity of F. oxysporum and suggest new potential targets for controlling fungal infections.

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A Colletotrichum fructicola dual specificity phosphatase CfMsg5 is regulated by the CfAp1 transcription factor during oxidative stress and promotes virulence on Camellia oleifera

Gao,

Zhang,

Sheng

et al. 2024

Virulence

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Anthracnose, caused by Colletotrichum species, induces significant economic damages to crop plants annually, especially for Camellia oleifera . During infection, the counter-defence mechanisms of plant pathogens against ROS-mediated resistance, however, remain poorly understood. By employing Weighted Gene Co-expression Network Analysis (WGCNA), we identified ACTIVATOR PROTEIN-1 (AP-1), a bZIP transcription factor, as significant to infection. And deletion of CfAP1 inhibited aerial hyphae formation and growth under oxidative stress. Furthermore, RNA-seq analysis post H 2 O 2 treatment revealed 33 significantly down-regulated genes in the AP-1 deficient strain, including A12032, a dual specificity phosphatase (DSP) homologous to MSG5 from Saccharomyces cerevisiae . This Δ Cfmsg5 strain showed enhanced oxidative tolerance, reduced ROS scavenging, and negative regulation of the CWI MAPK cascade under oxygen stress, suggesting its involvement in oxidative signal transduction. Importantly, we provide evidence that CfMsg5 regulates growth, endoplasmic reticulum stress, and several unfolded protein response genes upregulated in Δ Cfmsg5 . Collectively, this study identified core components during C. fructicola infection and highlights a potential regulatory module involving CfAp1 and CfMsg5 in response to host ROS bursts. It provides new insights into fungal infection mechanisms and potential targets like CfAP1 and CfMSG5 for managing anthracnose diseases.

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Dual-specificity protein phosphatase Msg5 controls cell wall integrity and virulence in Fusarium oxysporum

Cited by 7 publications

References 31 publications

Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Bacillus subtilis Strain Z-14 against Fusarium oxysporum Causing Vascular Wilt Diseases in Cucumber

Metabolomic and Transcriptome Analysis of the Inhibitory Effects of Bacillus subtilis Strain Z-14 against Fusarium oxysporum Causing Vascular Wilt Diseases in Cucumber

Constitutive activation of TORC1 signaling attenuates virulence in the cross-kingdom fungal pathogen Fusarium oxysporum

A Colletotrichum fructicola dual specificity phosphatase CfMsg5 is regulated by the CfAp1 transcription factor during oxidative stress and promotes virulence on Camellia oleifera

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