Dual-stimuli responsive i-motif/nanoflares for sensing ATP in lysosomes

Jin, Fen; Zheng, Jing; Liu, Changhui; Yang, Sheng; Li, Yinhui; Li, Jishan; Lian, Yan; Yang, Ronghua

doi:10.1039/c4an00153b

Cited by 18 publications

(16 citation statements)

References 28 publications

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“…Similarly, cyclic voltammetric detection of Cr(III) ions was also carried out for Cr(III) concentration ranging from 5x10 À4 M to 1.5x10 À2 M, using the microbe modified CPE in the pH range of 2 to 4. From the voltammograms portrayed in Figs.4(a-c) using the Cf-CPE at pH 2, 3 and 4 respectively, the LLOD for Cr(III) ions is found to be 5x10 À4 M. The slight shifts in the cathodic peak potentials observed as a function of Cr(VI) concentration, at different proton concentration/pH, has also been reported by other workers [35,[38][39][40].…”

Section: Cyclic Voltammetric Analysis Of Cr(vi)/cr(iii) Ions Using Cfsupporting

confidence: 82%

Electroanalytical Detection of Cr(VI) and Cr(III) Ions Using a Novel Microbial Sensor

et al. 2017

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A microbial sensor, namely carbon paste electrode (CPE) modified with Citrobacter freundii (Cf–CPE) has been developed for the detection of hexavalent (Cr(VI)) and trivalent (Cr(III)) chromium present in aqueous samples using voltammetry, an electroanalytical technique. The biosensor developed, demonstrated about a twofold higher performance as compared to the bare CPE for the chosen ions. Using cyclic voltammetry and by employing the fabricated Cf–CPE, the lowest limit of detection (LLOD) of 1x10−4 M and 5x10−4 M for Cr(VI) and Cr(III) ions respectively could be achieved. By adopting the Differential Pulse Cathodic Stripping Voltammetric technique, the LLOD could be further improved to 1x10−9 M and 1x10−7 M for Cr(VI) and Cr(III) ions respectively using the biomodified electrodes. The reactions occurring at the electrode surface‐chromium solution interface and the mechanisms of biosorption of chromium species onto the biosensor are discussed. The stability and utility of the developed biosensor for the analysis of Cr(VI) and Cr(III) ions in chromite mine water samples has been evaluated.

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Section: Cyclic Voltammetric Analysis Of Cr(vi)/cr(iii) Ions Using Cfsupporting

confidence: 82%

Electroanalytical Detection of Cr(VI) and Cr(III) Ions Using a Novel Microbial Sensor

et al. 2017

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“…Among various noble metal nanomaterials, gold nanomaterials are most intensely investigated as imaging contrast agents and nanocarriers . As a result, the integration of aptamer with gold nanomaterials, such as Au nanorods (AuNRs), Au nanoparticles, Au nanocages, Au nanostars (AuNSs), hollow gold nanospheres (HAuNS), and Au nanoclusters (Au NCs), has been widely employed in in vivo applications in diverse areas, such as intracellular molecular imaging, cancer cell and tumor imaging, targeted delivery of therapeutic cargos (e.g., anticancer drug, protein, and gene), and cancer therapy . In one example, Huang et al demonstrated the use of AuNRs as an efficient and robust multivalent platform for molecular assembly of aptamers for targeted cell imaging .…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

“…In addition to targeted cell imaging, aptamer‐based gold nanomaterials have also been used for intracellular molecular imaging. For instance, by combining the structure‐switching i‐motif and the highly selective adenosine triphosphate (ATP) aptamer, Jin et al developed a dual‐stimuli responsive i‐motif/nanoflares for ATP imaging in lysosomes . Later, Chen et al (Figure B) reported the use of polyA‐based SNA aptamer nanobeacons (PAaptNBs) that are functionalized with rationally designed polyadenine (polyA) diblock oligonucleotides through they achieved programmable engineering of PAaptNB for intracellular ATP imaging.…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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“…Their sensor enabled localization of both nucleotides, but not quantification (Wang Y. et al, 2013 ). Along the same lines, a multitude of sensing techniques was developed for the fluorescent detection of ATP in living cells in general (Tan et al, 2012 ; Wu C. et al, 2013 ; Liu et al, 2014 ; Jia et al, 2015 ; Qiang et al, 2015 ; Wang W. et al, 2015 ) and in lysosomes specifically (Jin et al, 2014 ). Yi et al went a step further and used the deeper penetration depth of two-photon microscopy to visualize ATP in zebrafish (Yi et al, 2014 ).…”

Section: Small Molecule Aptasensorsmentioning

confidence: 99%

Selection and Biosensor Application of Aptamers for Small Molecules

2016

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Small molecules play a major role in the human body and as drugs, toxins, and chemicals. Tools to detect and quantify them are therefore in high demand. This review will give an overview about aptamers interacting with small molecules and their selection. We discuss the current state of the field, including advantages as well as problems associated with their use and possible solutions to tackle these. We then discuss different kinds of small molecule aptamer-based sensors described in literature and their applications, ranging from detecting drinking water contaminations to RNA imaging.

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Dual-stimuli responsive i-motif/nanoflares for sensing ATP in lysosomes

Cited by 18 publications

References 28 publications

Electroanalytical Detection of Cr(VI) and Cr(III) Ions Using a Novel Microbial Sensor

Electroanalytical Detection of Cr(VI) and Cr(III) Ions Using a Novel Microbial Sensor

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Selection and Biosensor Application of Aptamers for Small Molecules

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