Dualité des corps protéiques du mésophylle adaxial et abaxial des cotylédons de quelques espèces de la tribu des Génistées (Légumineuses)

Citharel, L.; Citharel, J.

doi:10.1139/b87-256

Cited by 7 publications

(3 citation statements)

References 13 publications

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“…In the species Lupinus luteus, the abaxial face of the cotyledons is without any globoid in the PBs, whereas the adaxial face contains some globoids (Asghar and Dethason, 1992). A similar heterogeneity in the globoid distribution has also been reported to exist in Genistae (Citharel and Citharel, 1987). Calcium has been claimed to be a major ionic component of the PBs in seeds of Crambe abyssinica (Hofsten, 1973) and in the cotyledons of French beans (Geneves and Cachon, 1988;Vintejoux, 1992).…”

Section: Discussionmentioning

confidence: 89%

Imaging the K, Mg, Na and Ca Distributions in Flax Seeds using SIMS Microscopy

et al. 1994

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Taking advantage of the sensitivity, the lateral resolution and the ability of secondary ion mass spectrometry (SlMS) for elemental imaging, we have shown that the main alkaline and alkaline-earth cations exhibited clear-cut regionalization in flax seeds. Calcium, and even more magnesium were almost exclusively located within the protein bodies (PBs); and they were practically absent from the tissue areas with no PBs, such as the provascular tissue. By contrast, sodium was almost exclusively located outside the PBs; moreover, most of the seed sodium was in the tissues at the seed periphery (chiefly the teguments). Among the studied cations, potassium was the only one with a non-negligrble concentration both in and outside the PBs.

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Section: Discussionmentioning

confidence: 89%

Imaging the K, Mg, Na and Ca Distributions in Flax Seeds using SIMS Microscopy

et al. 1994

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“…1980). More recently, however, the presence of globoids in the PBs of several Leguminosae has been clearly established and it has been noticed that, as observed in C. siliquastrum, homogeneous and unhomogeneous PBs can be present in cells of different cotyledon regions (Citharel and Citharel. 1987;Asghar andDe Mason, 1990: Esnault et al, 1996).…”

Section: Resultsmentioning

confidence: 98%

Seed of Cercis siliquastrum L (Leguminosae, Caesalpinioideae): Reserves and their Utilization after Germination

et al. 1997

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An ultrastructural study on the reserves stored in cotyledons and endosperm and on their utilization after germination has been carried out in Cercis siliquastrum seeds. The observations have shown that all the cotyledonal reserves of proteins and lipids are used during seedling growth. However, inside the endosperm cells only the protein reserves are depleted, and large quantities of lipids remain undigested. In both cell walls and intercellular spaces of the endosperm cells huge masses of polysaccharide reserves are stored. However, only the polysaccharides inserted in the cell walls are dismantled, while those located in the intercellular spaces are not attacked by the lytic enzymes. Some hypotheses regarding the possible roles of those endospermic non-nutritional materials in the strategies of seed germination and seedling growth are suggested.

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“…Protein bodies differ in their structural complexity, and Rost (1972), and later Lott and Buttrose (1978), adopted a classification to describe the structural features of protein bodies within seeds. It has been generally considered that all species within the Fabaceae have only homogeneous protein bodies (Bain and Mercer, 1966;Opik, 1966;Miege, 1975;Pernollet, 1978;Weber and Neumann, 1980), although protein bodies with both protein crystalloid and globoid inclusions (heterogeneous) have been observed in cotyledons of pea (perner, 1965), lupin (LeGal and Rey, 1986), Cytisus (Citharel and Citharel, 1987), and Medicago sativa (Singh, 1977). In addition, a number of species within the tribe Genistae, such as Cytisus, are known to possess both homogeneous and heterogeneous protein bodies, which are spatially separated (Citharel and Citharel, 1987).…”

Section: Discussionmentioning

confidence: 99%

Developmental Changes in the Cotyledons of Lupinus luteus L. During and After Germination

Asghar¹,

DeMason²

1990

American Journal of Botany

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Cotyledons of Lupinus luteus were sampled from 1 to 21 days after sowing and processed for light microscopy and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). Length, width, and surface area of the cotyledons increased gradually until day 10. The thickness of the cotyledons increased from day 7 to day 12 and decreased thereafter. Morphometric analyses showed that the increase in length, width, and thickness of the cotyledon was due to cell expansion, and the decrease in thickness of the cotyledon was due to the decrease in the length of abaxial cells and in the total number of cells. Mesophyll development accompanied schizogenous and lysigenous air space formation. There were two structurally distinct types of protein bodies. Protein bodies in five to six layers of cells on the abaxial side did not contain globoids, while globoids were prominent in protein bodies in the center and adaxial side. Storage protein mobilization occurred first in the abaxial side of the cotyledon and proceeded toward the adaxial side. SDS‐PAGE analysis showed that proteins ranged from 97 to 14 kD. High molecular weight α‐ and ß‐conglutinins were more abundant in the abaxial region, whereas γ‐conglutinin occurred in both abaxial and adaxial regions. In addition, there were five minor bands between 97 and 43 kD unique to abaxial region and five minor bands between 43 and 14 kD unique to adaxial region in the nonreduced protein profiles. The α‐ and ß‐conglutinins began to decrease after imbibition and disappeared by day 7 after sowing. At this stage the subunits of ribulose‐l,5‐bisphosphate carboxylase/oxygenase and four new minor bands appeared.

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Dualité des corps protéiques du mésophylle adaxial et abaxial des cotylédons de quelques espèces de la tribu des Génistées (Légumineuses)

Cited by 7 publications

References 13 publications

Imaging the K, Mg, Na and Ca Distributions in Flax Seeds using SIMS Microscopy

Imaging the K, Mg, Na and Ca Distributions in Flax Seeds using SIMS Microscopy

Seed of Cercis siliquastrum L (Leguminosae, Caesalpinioideae): Reserves and their Utilization after Germination

Developmental Changes in the Cotyledons of Lupinus luteus L. During and After Germination

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