Dually crosslinked injectable alginate-based graft copolymer thermoresponsive hydrogels as 3D printing bioinks for cell spheroid growth and release

Saravanou, Sofia Falia; Ioannidis, Konstantinos; Dimopoulos, Andreas; Paxinou, Alexandra; Kounelaki, Fotoula; Varsami, Sevilli Maria; Tsitsilianis, Constantinos; Papantoniou, Ioannis; Pasparakis, George

doi:10.1016/j.carbpol.2023.120790

Cited by 27 publications

(21 citation statements)

References 45 publications

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“…Saravanou et al developed a multiple stimuli-responsive scaffold through grafting poly(N-isopropylacrylamide-co-N-tertbutylacrylamide) side chains to sodium alginate, which can be used as a bio ink and 3D-printed under mild conditions to form arbitrary geometries. [68] Firstly, a 3D network was prepared by the ionic interactions between negatively charged carboxyl groups in the main chain of alginate and positively charged Ca 2+ . Secondly, hydrophobic binding of thermoresponsive side chains could be achieved with heating, which thereby enhances the cross-linking density of the network.…”

Section: Thermoresponsive Dissolution Of Sacrificial Componentsmentioning

confidence: 99%

Hydrogel Scaffolds with Controlled Postgelation Modulation of Structures for 3D Cell Culture and Tissue Engineering

Yang,

Rong,

Chen

et al. 2023

Macro Chemistry & Physics

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Hydrogels are hydrophilic three‐dimensional networks containing a large amount of water, with physicochemical properties similar to extracellular matrix and controlled mechanical strength, making them ideal scaffolds for 3D cell culture and tissue engineering. However, the cross‐linked hydrogel network often restricts the migration of cells and the exchange of nutrients, which affects cell proliferation and the development of normal tissues. In recent years, hydrogels with pore‐channel structures have attracted significant attention, but these spatial structures are usually preconstructed before gelation, posing challenges in meeting the dynamic physiological conditions required during cell and tissue growth. Therefore, considerable efforts have been devoted to structurally regulate the scaffolds after gelation, so as to enhance the interactions between the scaffolds and cells for promoting the growth of cells and tissues. This review firstly outlines the preparation of hydrogel scaffolds with pore structure and the necessity of postgelation pore modulation. Two types of methods for postgelation pore modulation, including chemical degradation and physical dissolution, are then summarized. Finally, the potential application of such postgelation structural modulation in 3D cell culture and tissue engineering is discussed.

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Section: Thermoresponsive Dissolution Of Sacrificial Componentsmentioning

confidence: 99%

Hydrogel Scaffolds with Controlled Postgelation Modulation of Structures for 3D Cell Culture and Tissue Engineering

Yang,

Rong,

Chen

et al. 2023

Macro Chemistry & Physics

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“…However, a limitation of synthetic polymers is their lack of inherent cell adhesive motifs in their chemical structure, which can inhibit cell adhesion. Consequently, when implanted in vivo , these materials may trigger a significant inflammatory response. − …”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties

Chowdhury,

Mondal,

Ratnayake

et al. 2024

ACS Biomater. Sci. Eng.

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The compositional formulations and the optimization of process parameters to fabricate hydrogel scaffolds with urological tissue-mimicking biophysical properties are not yet extensively explored, including a comprehensive assessment of a spectrum of properties, such as mechanical strength, viscoelasticity, antimicrobial property, and cytocompatibility. While addressing this aspect, the present work provides mechanistic insights into process science, to produce shape-fidelity compliant alginate-based biomaterial ink blended with gelatin and synthetic nanocellulose. The composition-dependent pseudoplasticity, viscoelasticity, thixotropy, and gel stability over a longer duration in physiological context have been rationalized in terms of intermolecular hydrogen bonding interactions among the biomaterial ink constituents. By varying the hybrid hydrogel ink composition within a narrow compositional window, the resulting hydrogel closely mimics the natural urological tissue-like properties, including tensile stretchability, compressive strength, and biophysical properties. Based on the printability assessment using a critical analysis of gel strength, we have established the buildability of the acellular hydrogel ink and have been successful in fabricating shape-fidelity compliant urological patches or hollow cylindrical grafts using 3D extrusion printing. Importantly, the new hydrogel formulations with good hydrophilicity, support fibroblast cell proliferation and inhibit the growth of Gram-negative E. coli bacteria. These attributes were rationalized in terms of nanocellulose-induced physicochemical changes on the scaffold surface. Taken together, the present study uncovers the process-science-based understanding of the 3D extrudability of the newly formulated alginate-gelatin-nanocellulose-based hydrogels with urological tissue-specific biophysical, cytocompatibility, and antibacterial properties.

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“…20 The excellent mechanical properties of doublenetwork hydrogels are due to the first network providing dissipated energy during deformation and the other network providing hidden elasticity to maintain the integrity of the hydrogel. 21 Currently, various "green" materials derived from renewable resources have been used to fabricate multifunctional hydrogels, such as chitosan, 22,23 guar gum, 24 sodium alginate, 25 and agarose. 26 These natural polysaccharide-based hydrogels have multiple advantages such as good biocompatibility, low cost, and environmental protection and are widely used in the fabrication of flexible sensors.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Currently, various “green” materials derived from renewable resources have been used to fabricate multifunctional hydrogels, such as chitosan, , guar gum, sodium alginate, and agarose . These natural polysaccharide-based hydrogels have multiple advantages such as good biocompatibility, low cost, and environmental protection and are widely used in the fabrication of flexible sensors. − As a water-soluble derivative of chitosan, carboxymethyl chitosan (CMC) has many hydrophilic groups, which can enhance the mechanical properties of hydrogels .…”

Section: Introductionmentioning

confidence: 99%

Multifunctional, Self-Adhesive MXene-Based Hydrogel Flexible Strain Sensors for Hand-Written Digit Recognition with Assistance of Deep Learning

Zhang,

Luan

et al. 2023

Langmuir

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The conductive hydrogel as a flexible sensor not only has certain mechanical flexibility but also can be used in the field of human health detection and human−computer interaction. Herein, by introduction of tannic acid (TA) with MXene into the polyacrylamide (PAM)/carboxymethyl chitosan (CMC) doublenetwork hydrogel, a hydrogel with high stretchability, self-adhesion, and high sensitivity was prepared. CMC and PAM form a semiinterpenetrating double-network of high toughness and durability through electrostatic interactions and multiple hydrogen bonding networks. The abundant hydrophilic functional groups on TA and MXene form multiple hydrogen bonds simultaneously with the polymer network, ensuring high stretchability and sensitivity of the hydrogel. The hydrogel can display an accurate response to a variety of stimulus signals and can monitor both human joint movements and small physiological signal changes. It can also be combined with deep learning algorithms to classify handwritten digits with an accuracy rate of 98%. This work can promote the application of hydrogel sensors with durability and high sensitivity. The combination of algorithms and flexible sensors provides important ideas for the further development of flexible devices.

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Dually crosslinked injectable alginate-based graft copolymer thermoresponsive hydrogels as 3D printing bioinks for cell spheroid growth and release

Cited by 27 publications

References 45 publications

Hydrogel Scaffolds with Controlled Postgelation Modulation of Structures for 3D Cell Culture and Tissue Engineering

Hydrogel Scaffolds with Controlled Postgelation Modulation of Structures for 3D Cell Culture and Tissue Engineering

Three-Dimensional Extrusion Printed Urinary Specific Grafts: Mechanistic Insights into Buildability and Biophysical Properties

Multifunctional, Self-Adhesive MXene-Based Hydrogel Flexible Strain Sensors for Hand-Written Digit Recognition with Assistance of Deep Learning

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