Dually functional polyethylenimine-coated gold nanoparticles: a versatile material for electrode modification and highly sensitive simultaneous determination of four tumor markers

Putnin, Thitirat; Ngamaroonchote, Aroonsri; Wiriyakun, Natta; Ounnunkad, Kontad; Laocharoensuk, Rawiwan

doi:10.1007/s00604-019-3370-4

Cited by 32 publications

(16 citation statements)

References 34 publications

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“…In addition, Putnin prepared a sort of dually functional polyethylenimine (PEI)-coated gold nanoparticles as immunoprobes, using the strong electrostatic adsorption to loading of four different types of metal ions, including lead(II) ions, copper(II) ions, cadmium(II) ions, and silver ions [ 63 ]. On account of four independent voltammetric signals generated by these four metal ions, which were posited at −0.80, −0.55, −0.20, and +0.05 V, respectively, this multiplexed electrochemical immunosensor can efficaciously detect AFP, CEA, PSA, and interleukin-8 (IL-8).…”

Section: Electrochemical Signal Substances For Multiplexed Immunoassaymentioning

confidence: 99%

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Feng¹,

Chu²,

Ma³

2022

Molecules

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Appropriate labeling method of signal substance is necessary for the construction of multiplexed electrochemical immunosensing interface to enhance the specificity for the diagnosis of cancer. So far, various electrochemical substances, including organic molecules, metal ions, metal nanoparticles, Prussian blue, and other methods for an electrochemical signal generation have been successfully applied in multiplexed biosensor designing. However, few works have been reported on the summary of electrochemical signal substance applied in constructing multiplexed immunosensing interface. Herein, according to the classification of labeled electrochemical signal substance, this review has summarized the recent state-of-art development for the designing of electrochemical immunosensing interface for simultaneous detection of multiple tumor markers. After that, the conclusion and prospects for future applications of electrochemical signal substances in multiplexed immunosensors are also discussed. The current review can provide a comprehensive summary of signal substance selection for workers researched in electrochemical sensors, and further, make contributions for the designing of multiplexed electrochemical immunosensing interface with well signal.

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Section: Electrochemical Signal Substances For Multiplexed Immunoassaymentioning

confidence: 99%

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Feng¹,

Chu²,

Ma³

2022

Molecules

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“…Error bars showed the standard deviation (SD) of five detections. The LOD of E. coli O157:H7 analyzed using the LFIA strips was 50 cells/mL based on the IUPAC formula (LOD = y blank + 3 × SD blank , where y blank and SD blank are the average Raman intensity and the SD of the blank control, respectively) (Hu et al, 2017;Putnin et al, 2019). The SiO 2 /Au-based SERS-LFIA strips were characterized by SEM to prove that the SERS signal came from the SERS tags fixed on the test line.…”

Section: Optimization Of Sers-based Lfia Stripsmentioning

confidence: 99%

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Shi

Xiao

et al. 2020

Front. Microbiol.

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Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapiddetection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO 2 /Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare highperformance tags for the SERS-based LFIA system. The SiO 2 /Au SERS tags, which were modified with two layers of Raman reporter molecules and monoclonal antibodies, effectively bind with E. coli O157:H7 and form sandwich immune complexes on the test lines. E. coli O157:H7 was quantitatively detected easily by detecting the Raman intensity of the test lines. Under optimal conditions, the limit of detection (LOD) of the SiO 2 /Au-based SERS-LIFA strips for the target bacteria was 50 cells/mL in PBS solution, indicating these strips are 2,000 times more sensitive than colloidal Au-based LFIA strips. Moreover, the proposed assay demonstrated high applicability in E. coli O157:H7 detection in biological samples, including tap water, milk, human urine, lettuce extract and beef, with a low LOD of 100 cells/mL. Results indicate that the proposed SERS-based LFIA strip is applicable for the sensitive and quantitative determination of E. coli O157:H7.

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“…DPV was used for the detection of the oxidation peaks of Cu, Zn, and Pb ions obtained by acid dissolution of the hybrid NPS at +0.8, +1.3, and +0.1 V, respectively [ 78 ] A similar study was done by Putnin et al with polyethylenimine-coated gold nanoparticles (PEI-AuNPs) conjugated with four different electroactive metal ions—Cd (II), Ag (I), Pb (II), and Cu (II)—as labels. They also used the same PEI-AuNPs for surface modification of the SPEs and detected four tumor biomarkers—PSA, AFP, CEA, and interleukin-8 (IL-8)—with LODs from 0.9 to 1.7 fg mL −1 [ 79 ].…”

Section: Immunoassay Techniques For Printed Electrodesmentioning

confidence: 99%

“…Their findings indicate larger surface areas hinder reaching ultrasensitive measurements and suppression of the background signal can easily be achieved by minimal surface area [ 139 ]. We have already presented many examples of signal amplification by surface modification of SPE’s by nanoparticles [ 17 , 79 , 90 , 91 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 109 , 110 , 111 ]. A few more examples are included in this section.…”

Section: Screen Printingmentioning

confidence: 99%

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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Dually functional polyethylenimine-coated gold nanoparticles: a versatile material for electrode modification and highly sensitive simultaneous determination of four tumor markers

Cited by 32 publications

References 34 publications

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Electrochemical Signal Substance for Multiplexed Immunosensing Interface Construction: A Mini Review

Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

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